Cytochrome P450 expression system for high-throughput real-time detection of genotoxicity: Application to the study of human CYP1A2 variants

2016 ◽  
Vol 806 ◽  
pp. 24-33 ◽  
Author(s):  
Bernardo Brito Palma ◽  
Daniela Moutinho ◽  
Philippe Urban ◽  
José Rueff ◽  
Michel Kranendonk
Keyword(s):  
Cytochrome P450 ◽  
Real Time ◽  
High Throughput ◽  
Expression System ◽  
P450 Expression ◽  
Real Time Detection ◽  
Human Cyp1a2

Human cytochrome P450 expression in bacteria: Whole-cell high-throughput activity assay for CYP1A2, 2A6 and 3A4

2018 ◽  
Vol 158 ◽  
pp. 134-140 ◽  
Author(s):  
Francisco Esteves ◽  
Diana Campelo ◽  
Philippe Urban ◽  
Sophie Bozonnet ◽  
Thomas Lautier ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
High Throughput ◽  
Activity Assay ◽  
Whole Cell ◽  
Human Cytochrome ◽  
P450 Expression

scholarly journals The stimulatory role of human cytochrome b5 in the bioactivation activities of human CYP1A2, 2A6 and 2E1: a new cell expression system to study cytochrome P450 mediated biotransformation

Mutagenesis ◽  
2005 ◽  
Vol 20 (2) ◽  
pp. 93-100 ◽  
Author(s):  
Maria Paula Duarte ◽  
Bernardo Brito Palma ◽  
Andrei A. Gilep ◽  
António Laires ◽  
José Santos Oliveira ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Expression System ◽  
Cytochrome B5 ◽  
Human Cytochrome ◽  
Cell Expression ◽  
Human Cyp1a2 ◽  
Cell Expression System

Real-time monitoring of small biological molecules by ligation-mediated polymerase chain reaction

2015 ◽  
Vol 51 (61) ◽  
pp. 12270-12273 ◽  
Author(s):  
Yan Zhang ◽  
Jiao Liu ◽  
Chun-yang Zhang
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
High Throughput ◽  
Biological Molecules ◽  
Real Time Monitoring ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Real Time Detection

We develop a ligation-mediated polymerase chain reaction (PCR) for real-time detection of small biological molecules in a high-throughput format.

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