BMP-2 gene transfection of bone marrow stromal cells to induce osteoblastic differentiation in a rat calvarial defect model

2018 ◽  
Vol 91 ◽  
pp. 806-816 ◽  
Author(s):  
Ming-Kai Hsieh ◽  
Chia-Jung Wu ◽  
Chun-Chieh Chen ◽  
Tsung-Ting Tsai ◽  
Chi-Chien Niu ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Gene Transfection ◽  
Osteoblastic Differentiation ◽  
Marrow Stromal Cells ◽  
Calvarial Defect ◽  
Defect Model ◽  
Rat Calvarial Defect

scholarly journals Endochondral Ossification Induced by Cell Transplantation of Endothelial Cells and Bone Marrow Stromal Cells with Copolymer Scaffold Using a Rat Calvarial Defect Model

Polymers ◽  
2021 ◽  
Vol 13 (9) ◽  
pp. 1521
Author(s):  
Zhe Xing ◽  
Xiaofeng Jiang ◽  
Qingzong Si ◽  
Anna Finne-Wistrand ◽  
Bin Liu ◽  
...  
Keyword(s):  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Host Response ◽  
Endochondral Ossification ◽  
Marrow Stromal Cells ◽  
Calvarial Defect ◽  
Defect Model ◽  
Calvarial Defects ◽  
Ossification Process ◽  
Rat Calvarial Defect

It has been recently reported that, in a rat calvarial defect model, adding endothelial cells (ECs) to a culture of bone marrow stromal cells (BMSCs) significantly enhanced bone formation. The aim of this study is to further investigate the ossification process of newly formed osteoid and host response to the poly(L-lactide-co-1,5-dioxepan-2-one) [poly(LLA-co-DXO)] scaffolds based on previous research. Several different histological methods and a PCR Array were applied to evaluate newly formed osteoid after 8 weeks after implantation. Histological results showed osteoid formed in rat calvarial defects and endochondral ossification-related genes, such as dentin matrix acidic phosphoprotein 1 (Dmp1) and collagen type II, and alpha 1 (Col2a1) exhibited greater expression in the CO (implantation with BMSC/EC/Scaffold constructs) than the BMSC group (implantation with BMSC/Scaffold constructs) as demonstrated by PCR Array. It was important to notice that cartilage-like tissue formed in the pores of the copolymer scaffolds. In addition, multinucleated giant cells (MNGCs) were observed surrounding the scaffold fragments. It was concluded that the mechanism of ossification might be an endochondral ossification process when the copolymer scaffolds loaded with co-cultured ECs/BMSCs were implanted into rat calvarial defects. MNGCs were induced by the poly(LLA-co-DXO) scaffolds after implantation, and more specific in vivo studies are needed to gain a better understanding of host response to copolymer scaffolds.

Characterization of human bone marrow stromal cells with respect to osteoblastic differentiation

1997 ◽  
Vol 15 (4) ◽  
pp. 546-557 ◽  
Author(s):  
Alana K. Majors ◽  
Cynthia A Boehm ◽  
Hironori Nitto ◽  
Ronald J. Midura ◽  
George F. Muschler
Keyword(s):  
Bone Marrow ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Osteoblastic Differentiation ◽  
Marrow Stromal Cells ◽  
Human Bone ◽  
Human Bone Marrow
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