Tigogenin inhibits adipocytic differentiation and induces osteoblastic differentiation in mouse bone marrow stromal cells

2007 ◽  
Vol 270 (1-2) ◽  
pp. 17-22 ◽  
Author(s):  
Hua Zhou ◽  
Xi Yang ◽  
Naili Wang ◽  
Yaou Zhang ◽  
Guoping Cai
Keyword(s):  
Bone Marrow ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Osteoblastic Differentiation ◽  
Marrow Stromal Cells ◽  
Mouse Bone Marrow ◽  
Adipocytic Differentiation

scholarly journals Identification of a clinical signature predictive of differentiation fate of human bone marrow stromal cells

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Justyna Magdalena Kowal ◽  
Sören Möller ◽  
Dalia Ali ◽  
Florence Figeac ◽  
Torben Barington ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Vitamin D ◽  
Bone Regeneration ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Osteoblastic Differentiation ◽  
Marrow Stromal Cells ◽  
Human Bone ◽  
Differentiation Capacity ◽  
Adipocytic Differentiation

Abstract Background Transplantation of human bone marrow stromal cells (hBMSCs) is a promising therapy for bone regeneration due to their ability to differentiate into bone forming osteoblastic cells. However, transplanted hBMSCs exhibit variable capacity for bone formation resulting in inconsistent clinical outcome. The aim of the study was to identify a set of donor- and cell-related characteristics that detect hBMSCs with optimal osteoblastic differentiation capacity. Methods We collected hBMSCs from 58 patients undergoing surgery for bone fracture. Clinical profile of the donors and in vitro characteristics of cultured hBMSCs were included in uni- and multivariable analysis to determine their predictive value for osteoblastic versus adipocytic differentiation capacity assessed by quantification of mineralized matrix and mature adipocyte formation, respectively. Results We identified a signature that explained > 50% of variation in osteoblastic differentiation outcome which included the following positive predictors: donor sex (male), absence of osteoporosis diagnosis, intake of vitamin D supplements, higher fraction of CD146+, and alkaline phosphate (ALP+) cells. With the exception of vitamin D and ALP+ cells, these variables were also negative predictors of adipocytic differentiation. Conclusions Using a combination of clinical and cellular criteria, it is possible to predict differentiation outcome of hBMSCs. This signature may be helpful in selecting donor cells in clinical trials of bone regeneration.

Induction of Neuron-Specific Enolase Promoter and Neuronal Markers in Differentiated Mouse Bone Marrow Stromal Cells

2003 ◽  
Vol 21 (2) ◽  
pp. 121-132 ◽  
Author(s):  
Yossef S. Levy ◽  
Doron Merims ◽  
Hanna Panet ◽  
Yael Barhum ◽  
Eldad Melamed ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Neuron Specific Enolase ◽  
Marrow Stromal Cells ◽  
Mouse Bone Marrow ◽  
Neuronal Markers

scholarly journals Effects of Low-Intensity Electromagnetic Fields on the Proliferation and Differentiation of Cultured Mouse Bone Marrow Stromal Cells

2012 ◽  
Vol 92 (9) ◽  
pp. 1208-1219 ◽  
Author(s):  
Cheng Zhong ◽  
Xin Zhang ◽  
Zhengjian Xu ◽  
Rongxin He
Keyword(s):  
Bone Marrow ◽  
Cell Proliferation ◽  
Electromagnetic Fields ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Marrow Stromal Cells ◽  
Control Group ◽  
Mouse Bone Marrow ◽  
Low Intensity ◽  
Hematoxylin And Eosin

Background Electromagnetic fields (EMFs) used in stem-cell tissue engineering can help elucidate their biological principles. Objective The aim of this study was to investigate the effects of low-intensity EMFs on cell proliferation, differentiation, and cycle in mouse bone marrow stromal cells (BMSCs) and the in vivo effects of EMFs on BMSC. Methods Harvested BMSCs were cultured for 3 generations and divided into 4 groups. The methylthiotetrazole (MTT) assay was used to evaluate cell proliferation, and alkaline phosphatase activity was measured via a colorimetric assay on the 3rd, 7th, and 10th days. Changes in cell cycle also were analyzed on the 7th day, and bone nodule formation was analyzed on the 12th day. Additionally, the expression of the collagen I gene was examined by reverse transcription-polymerase chain reaction (RT-PCR) on the 10th day. The BMSCs of the irradiated group and the control group were transplanted into cortical bone of different mice femurs separately, with poly(lactic-co-glycolic acid) (PLGA) serving as a scaffold. After 4 and 8 weeks, bone the bone specimens of mice were sliced and stained by hematoxylin and eosin separately. Results The results showed that EMFs (0.5 mT, 50 Hz) accelerated cellular proliferation, enhanced cellular differentiation, and increased the percentage of cells in the G2/M+S (postsynthetic gap 2 period/mitotic phase + S phase) of the stimulation. The EMF-exposed groups had significantly higher collagen I messenger RNA levels than the control group. The EMF + osteogenic medium–treated group readily formed bone nodules. Hematoxylin and eosin staining showed a clear flaking of bone tissue in the irradiated group. Conclusion Irradiation of BMSCs with low-intensity EMFs (0.5 mT, 50 Hz) increased cell proliferation and induced cell differentiation. The results of this study did not establish a stricter animal model for studying osteogenesis, and only short-term results were investigated. Further study of the mechanism of EMF is needed.

Dose-Dependent Osteogenic Effect of Octacalcium Phosphate on Mouse Bone Marrow Stromal Cells

2008 ◽  
Vol 0 (0) ◽  
pp. 080422095744451 ◽  
Author(s):  
Takahisa Anada ◽  
Takashi Kumagai ◽  
Yoshitomo Honda ◽  
Taisuke Masuda ◽  
Ryutaro Kamijo ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Octacalcium Phosphate ◽  
Marrow Stromal Cells ◽  
Mouse Bone Marrow ◽  
Osteogenic Effect ◽  
Dose Dependent

Europium-doped NaYF4 nanoparticles cause the necrosis of primary mouse bone marrow stromal cells through lysosome damage

RSC Advances ◽  
2016 ◽  
Vol 6 (26) ◽  
pp. 21725-21734 ◽  
Author(s):  
Kun Ge ◽  
Wentong Sun ◽  
Shaohan Zhang ◽  
Shuxian Wang ◽  
Guang Jia ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Marrow Stromal Cells ◽  
Mouse Bone Marrow ◽  
Primary Mouse ◽  
Biomedical Fields

Applications of europium-doped NaYF4 (NaYF4:Eu3+) nanoparticles in biomedical fields will inevitably increase their exposure to humans, therefore, the assessment of toxicities must be taken into consideration.

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