Direct three-dimensional imaging for morphological analysis of electrospun fibers with laboratory-based Zernike X-ray phase-contrast computed tomography

2020 ◽  
Vol 115 ◽  
pp. 111045 ◽  
Author(s):  
Cristine Santos de Oliveira ◽  
Adriana Trompetero González ◽  
Tobias Hedtke ◽  
Tobias Kürbitz ◽  
Andreas Heilmann ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Phase Contrast ◽  
Morphological Analysis ◽  
Three Dimensional ◽  
Electrospun Fibers ◽  
Three Dimensional Imaging ◽  
X Ray

Visualization of microvasculature and thrombi by X-ray phase-contrast computed tomography in hepatocellular carcinoma

2016 ◽  
Vol 23 (2) ◽  
pp. 600-605 ◽  
Author(s):  
Jianbo Jian ◽  
Hao Yang ◽  
Xinyan Zhao ◽  
Ruijiao Xuan ◽  
Yujie Zhang ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Hepatocellular Carcinoma ◽  
Phase Contrast ◽  
Soft Tissues ◽  
Three Dimensional ◽  
X Ray ◽  
Density Distributions ◽  
Fine Structures ◽  
Tumor Growth And Metastasis ◽  
Micrometer Scale

Visualization of the microvascular network and thrombi in the microvasculature is a key step to evaluating the development of tumor growth and metastasis, and influences treatment selection. X-ray phase-contrast computed tomography (PCCT) is a new imaging technique that can detect minute changes of density and reveal soft tissues discrimination at micrometer-scale resolution. In this study, six human resected hepatocellular carcinoma (HCC) tissues were investigated with PCCT. A histological stain was added to estimate the accuracy of PCCT. The results showed that the fine structures of the microvasculature (measuring 30–100 µm) and thrombi in tiny blood vessels were displayed clearly on imaging the HCC tissues by PCCT. Moreover, density distributions of the thrombi were obtained, which could be reliably used to distinguish malignant from benign thrombi in HCC. In conclusion, PCCT can clearly show the three-dimensional subtle structures of HCC that cannot be detected by conventional absorption-based computed tomography and provides a new method for the imageology of HCC.

scholarly journals Three-Dimensional Imaging of Nerve Tissue by X-Ray Phase-Contrast Microtomography

1999 ◽  
Vol 76 (1) ◽  
pp. 98-102 ◽  
Author(s):  
F. Beckmann ◽  
K. Heise ◽  
B. Kölsch ◽  
U. Bonse ◽  
M.F. Rajewsky ◽  
...  
Keyword(s):  
Phase Contrast ◽  
Three Dimensional ◽  
Nerve Tissue ◽  
Three Dimensional Imaging ◽  
X Ray

scholarly journals X-ray phase-contrast computed tomography visualizes the microstructure and degradation profile of implanted biodegradable scaffolds after spinal cord injury

2015 ◽  
Vol 22 (1) ◽  
pp. 136-142 ◽  
Author(s):  
Kenta Takashima ◽  
Masato Hoshino ◽  
Kentaro Uesugi ◽  
Naoto Yagi ◽  
Shojiro Matsuda ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Spinal Cord ◽  
Tissue Engineering ◽  
Phase Contrast ◽  
Three Dimensional ◽  
Injured Spinal Cord ◽  
X Ray ◽  
Biodegradable Scaffolds ◽  
Cord Injury ◽  
Grating Interferometer

Tissue engineering strategies for spinal cord repair are a primary focus of translational medicine after spinal cord injury (SCI). Many tissue engineering strategies employ three-dimensional scaffolds, which are made of biodegradable materials and have microstructure incorporated with viable cells and bioactive molecules to promote new tissue generation and functional recovery after SCI. It is therefore important to develop an imaging system that visualizes both the microstructure of three-dimensional scaffolds and their degradation process after SCI. Here, X-ray phase-contrast computed tomography imaging based on the Talbot grating interferometer is described and it is shown how it can visualize the polyglycolic acid scaffold, including its microfibres, after implantation into the injured spinal cord. Furthermore, X-ray phase-contrast computed tomography images revealed that degradation occurred from the end to the centre of the braided scaffold in the 28 days after implantation into the injured spinal cord. The present report provides the first demonstration of an imaging technique that visualizes both the microstructure and degradation of biodegradable scaffolds in SCI research. X-ray phase-contrast imaging based on the Talbot grating interferometer is a versatile technique that can be used for a broad range of preclinical applications in tissue engineering strategies.

Three dimensional imaging of laser-imploded targets using X-ray computed tomography technique

1997 ◽  
Vol 44 (3) ◽  
pp. 890-893 ◽  
Author(s):  
Y.-W. Chen ◽  
N. Miyanafa ◽  
M. Yamanaka ◽  
M. Nakai ◽  
S. Nakai
Keyword(s):  
Computed Tomography ◽  
Three Dimensional ◽  
Three Dimensional Imaging ◽  
X Ray ◽  
X Ray Computed

High-Resolution Radiography for Detecting and Measuring Micron-Scale Features

2004 ◽  
Author(s):  
Daniel H. Morse ◽  
Arlyn J. Antolak ◽  
Bernice E. Mills
Keyword(s):  
Computed Tomography ◽  
Composite Materials ◽  
Phase Contrast ◽  
Three Dimensional ◽  
Conventional Radiography ◽  
Small Scale ◽  
Micro Computed Tomography ◽  
Contrast Imaging ◽  
X Ray ◽  
Projection Radiography

X-ray radiography has long been recognized as a valuable tool for detecting internal features and flaws. Recent developments in microfabrication and composite materials have extended inspection requirements to the resolution limits of conventional radiography. Our work has been directed toward pushing both detection and measurement capabilities to a smaller scale. Until recently, we have used conventional contact radiography, optimized to resolve small features. With the recent purchase of a nano-focus (sub-micron) x-ray source, we are now investigating projection radiography, phase contrast imaging and micro-computed tomography (μ-CT). Projection radiography produces a magnified image that is limited in spatial resolution mainly by the source size, not by film grain size or detector pixel size. Under certain conditions phase contrast can increase the ability to resolve small features such as cracks, especially in materials with low absorption contrast. Micro-computed tomography can provide three-dimensional measurements on a micron scale and has been shown to provide better sensitivity than simple radiographs. We have included applications of these techniques to small-scale measurements not easily made by mechanical or optical means. Examples include void detection in meso-scale nickel MEMS parts, measurement of edge profiles in thick gold lithography masks, and characterization of the distribution of phases in composite materials. Our work, so far, has been limited to film.

Three-dimensional imaging of whole mouse models: comparing nondestructive X-ray phase-contrast micro-CT with cryotome-based planar epi-illumination imaging

2013 ◽  
Vol 253 (1) ◽  
pp. 24-30 ◽  
Author(s):  
A. TAPFER ◽  
M. BECH ◽  
I. ZANETTE ◽  
P. SYMVOULIDIS ◽  
S. STANGL ◽  
...  
Keyword(s):  
Mouse Models ◽  
Phase Contrast ◽  
Three Dimensional ◽  
Micro Ct ◽  
Three Dimensional Imaging ◽  
X Ray

scholarly journals Direct information retrieval after 3D reconstruction in grating-based X-ray phase-contrast computed tomography

2018 ◽  
Vol 25 (4) ◽  
pp. 1222-1228 ◽  
Author(s):  
Zhao Wu ◽  
Kun Gao ◽  
Zhili Wang ◽  
Chenxi Wei ◽  
Faiz Wali ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Information Retrieval ◽  
Phase Contrast ◽  
Three Dimensional ◽  
Large Field ◽  
Computational Time ◽  
Imaging Method ◽  
Phase Reconstruction ◽  
Phase Information ◽  
X Ray

Grating-based X-ray differential phase-contrast imaging has attracted a great amount of attention and has been considered as a potential imaging method in clinical medicine because of its compatibility with the traditional X-ray tube source and the possibility of a large field of view. Moreover, phase-contrast computed tomography provides three-dimensional phase-contrast visualization. Generally, two-dimensional information retrieval performed on every projection is required prior to three-dimensional reconstruction in phase-contrast computed tomography. In this paper, a three-dimensional information retrieval method to separate absorption and phase information directly from two reconstructed images is derived. Theoretical derivations together with numerical simulations have been performed to confirm the feasibility and veracity of the proposed method. The advantages and limitations compared with the reverse projection method are also discussed. Owing to the reduced data size and the absence of a logarithm operation, the computational time for information retrieval is shortened by the proposed method. In addition, the hybrid three-dimensional images of absorption and phase information were reconstructed using an absorption reconstruction algorithm, hence the existing data pre-processing methods and iterative reconstruction algorithms in absorption reconstruction may be utilized in phase reconstruction immediately.

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