Recombinant human interferon alpha-2β: Cloning, expression and PAT-process analytical technology enabled production in glycoengineered Pichia pastoris

New Biotechnology ◽

10.1016/j.nbt.2018.05.112 ◽

2018 ◽

Vol 44 ◽

pp. S35

Author(s):

S. Katla ◽

N. Mohan ◽

K. Yoganand ◽

B. Anand ◽

S. Sivaprakasam

Keyword(s):

Pichia Pastoris ◽

Interferon Alpha ◽

Process Analytical Technology ◽

Human Interferon ◽

Analytical Technology

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Control of specific growth rate for the enhanced production of human interferon α2b in glycoengineered Pichia pastoris : process analytical technology guided approach

Journal of Chemical Technology & Biotechnology ◽

10.1002/jctb.6118 ◽

2019 ◽

Vol 94 (10) ◽

pp. 3111-3123 ◽

Author(s):

Srikanth Katla ◽

Naresh Mohan ◽

Satya S Pavan ◽

Uttariya Pal ◽

Senthilkumar Sivaprakasam

Keyword(s):

Growth Rate ◽

Pichia Pastoris ◽

Specific Growth Rate ◽

Specific Growth ◽

Process Analytical Technology ◽

Human Interferon ◽

Enhanced Production ◽

Analytical Technology ◽

Interferon Α2b

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Anwendung spektroskopischer Messverfahren und Multivariater Datenanalyse zur Bewertung und Beobachtung von Bioprozessen

10.51202/9783186293176 ◽

2017 ◽

Author(s):

Jan-Patrick Voß

Keyword(s):

Pichia Pastoris ◽

Least Squares ◽

Support Vector Regression ◽

Partial Least Squares ◽

Partial Least Squares Regression ◽

Process Analytical Technology ◽

Support Vector ◽

Least Squares Regression ◽

Analytical Technology

Die vorliegende Arbeit befasst sich mit dem Bioprozessmonitoring unter Verwendung spektroskopischer Messverfahren und multivariater Datenanalyse nach den Grundsätzen von PAT – Process Analytical Technology. Mit NIR-Spektroskopie und dem Verfahren Soft Independent Modelling of Class Analogy (SIMCA) wurde eine Quali¬tätsbewertung von Hefeextrakten realisiert. Im Vordergrund stand jedoch die Quanti¬fizierung nicht direkt messbarer Größen aus NIR-, Raman- und 2D-Fluoreszenzspektren in pharmazeutischen Produktionsprozessen mit Pichia pastoris. Eine entsprechende Online-Bestimmung mit der Methode Partial Least Squares Regression (PLSR) kam weiterführend zur Regelung der Glycerolkonzentration zum Einsatz. Darüber hinaus wurde die Verwendung nichtspektraler Online-Daten zur Prozessbeobachtung erprobt. Dabei gelang mit Hilfe des nichtlinearen Verfahrens Support Vector Regression (SVR) unter anderem die Bestimmung zellspezifischer Reaktionsraten. ...

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Comprehensive characterization of recombinant Pichia pastoris using dynamic process conditions and process analytical technology

New Biotechnology ◽

10.1016/j.nbt.2009.06.143 ◽

2009 ◽

Vol 25 ◽

pp. S204-S205 ◽

Author(s):

D.V. Rial ◽

M. García Torreiro ◽

C. Dietzsch ◽

P. Wechselberger ◽

C. Herwig

Keyword(s):

Pichia Pastoris ◽

Dynamic Process ◽

Process Analytical Technology ◽

Process Conditions ◽

Recombinant Pichia Pastoris ◽

Analytical Technology ◽

Comprehensive Characterization

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Novel glycosylated human interferon alpha 2b expressed in glycoengineered Pichia pastoris and its biological activity: N-linked glycoengineering approach

Enzyme and Microbial Technology ◽

10.1016/j.enzmictec.2019.05.007 ◽

2019 ◽

Vol 128 ◽

pp. 49-58 ◽

Author(s):

Srikanth Katla ◽

K.N.R. Yoganand ◽

Smita Hingane ◽

C.T. Ranjith Kumar ◽

B. Anand ◽

...

Keyword(s):

Biological Activity ◽

Pichia Pastoris ◽

Interferon Alpha ◽

Human Interferon ◽

Interferon Alpha 2B

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Purification and Stability Expression of Open Reading Frames encoding Fusion and Non Fusion Human Interferon Alpha-2a produced in Methilotropic Yeast Pichia pastoris

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/439/1/012026 ◽

2020 ◽

Vol 439 ◽

pp. 012026

Author(s):

R A Ningrum ◽

A Z Mustopa ◽

D F Agustiyanti ◽

A T Fathurahman ◽

S Swasthikawati

Keyword(s):

Pichia Pastoris ◽

Interferon Alpha ◽

Open Reading Frames ◽

Human Interferon ◽

Yeast Pichia Pastoris ◽

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Overproduction, characterization and preliminary antiproliferative activity determination of non-tagged recombinant human interferon alpha-2a produced in Pichia pastoris

Nusantara Bioscience ◽

10.13057/nusbiosci/n090117 ◽

2017 ◽

Vol 9 (1) ◽

Author(s):

NENG HERAWATI

Keyword(s):

Pichia Pastoris ◽

Interferon Alpha ◽

Antiproliferative Activity ◽

Human Interferon ◽

Activity Determination

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Development of Higher Molecular Weight of Recombinant Human Interferon Alpha-2a by Albumin Fusion Technology in Methilotropic Yeast Pichia Pastoris

International Journal on Advanced Science Engineering and Information Technology ◽

10.18517/ijaseit.6.5.912 ◽

2017 ◽

Vol 7 (1) ◽

pp. 8 ◽

Author(s):

Ratih Asmana Ningrum ◽

Neng Herawati ◽

Andri Wardiana

Keyword(s):

Molecular Weight ◽

Pichia Pastoris ◽

Interferon Alpha ◽

Human Interferon ◽

Fusion Technology ◽

Yeast Pichia Pastoris

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Process Analytical Technology in Bioprocess Development and Manufacturing

Comprehensive Biotechnology ◽

10.1016/b978-0-444-64046-8.00183-x ◽

2011 ◽

pp. 535-544

Author(s):

J.C. Menezes

Keyword(s):

Process Analytical Technology ◽

Bioprocess Development ◽

Analytical Technology

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Specific residues within an amino-terminal domain of 35 residues of interferon alpha are responsible for recognition of the human interferon alpha cell receptor and for triggering biological effects.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(18)45560-3 ◽

1987 ◽

Vol 262 (13) ◽

pp. 6227-6237 ◽

Author(s):

A. Shafferman ◽

B. Velan ◽

S. Cohen ◽

M. Leitner ◽

H. Grosfeld

Keyword(s):

Interferon Alpha ◽

Biological Effects ◽

Cell Receptor ◽

Human Interferon ◽

Amino Terminal ◽

Terminal Domain ◽

Amino Terminal Domain

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Exploiting Fluorescent Reporter Molecules for Process Analytical Technology (PAT)

CHIMIA International Journal for Chemistry ◽

10.2533/chimia.2009.171 ◽

2009 ◽

Vol 63 (3) ◽

pp. 171-173 ◽

Author(s):

Tobias Broger ◽

Res P. Odermatt ◽

Pablo Ledergerber ◽

Bernhard Sonnleitner

Keyword(s):

Process Analytical Technology ◽

Fluorescent Reporter ◽

Analytical Technology

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