Metabolomic profiling in inner ear fluid by gas chromatography/mass spectrometry in guinea pig cochlea

2015 ◽  
Vol 606 ◽  
pp. 188-193 ◽  
Author(s):  
Takeshi Fujita ◽  
Daisuke Yamashita ◽  
Yasuhiro Irino ◽  
Junko Kitamoto ◽  
Yuriko Fukuda ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Guinea Pig ◽  
Inner Ear ◽  
Gas Chromatography Mass Spectrometry ◽  
Metabolomic Profiling ◽  
Guinea Pig Cochlea ◽  
Chromatography Mass Spectrometry

scholarly journals Identification of Biomarkers of Stent Restenosis With Serum Metabolomic Profiling Using Gas Chromatography/Mass Spectrometry

2012 ◽  
Vol 76 (8) ◽  
pp. 1864-1873 ◽  
Author(s):  
Minoru Hasokawa ◽  
Masakazu Shinohara ◽  
Hiroshi Tsugawa ◽  
Takeshi Bamba ◽  
Eiichiro Fukusaki ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Gas Chromatography Mass Spectrometry ◽  
Stent Restenosis ◽  
Metabolomic Profiling ◽  
Chromatography Mass Spectrometry

scholarly journals Metabolomic Profiling of Human Spermatozoa in Idiopathic Asthenozoospermia Patients Using Gas Chromatography-Mass Spectrometry

2018 ◽  
Vol 2018 ◽  
pp. 1-8 ◽  
Author(s):  
Kai Zhao ◽  
Jianzhong Zhang ◽  
Zhen Xu ◽  
Yue Xu ◽  
Aiming Xu ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Krebs Cycle ◽  
Human Sperm ◽  
Gas Chromatography Mass Spectrometry ◽  
Sperm Cells ◽  
Spectrometric Data ◽  
Metabolomic Profiling ◽  
Chromatography Mass Spectrometry ◽  
First Time

The purpose of this study was to describe the first metabolic profile of human sperm cells through the application of an untargeted platform based on gas chromatography-mass spectrometry (GC-MS). Sperm cell samples from patients diagnosed with idiopathic asthenozoospermia (n=30) and healthy subjects (n=30) were analyzed using a nontargeted metabolomics method based on GC-MS spectroscopy. The mass spectrometric data were collected using multivariate and univariate analyses to identify metabolites related to idiopathic asthenozoospermia. By using metabolomic strategies, we identified 33 metabolites, 27 of which were decreased in the idiopathic asthenozoospermia group compared with the normozoospermic group and six were increased in idiopathic asthenozoospermia. With respect to human sperm cells, some of these metabolites are reported here for the first time. Pathways for nucleoside, amino acid and energy metabolism, and the Krebs cycle were disturbed and were associated with idiopathic asthenozoospermia. The metabolic profiling provides an important first step in studying the pathophysiological mechanisms involved in IAS, and the identified metabolites may become potential biomarkers for its diagnosis and treatment.

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