C6. Effect of different concentrations of L-arginine on proliferation of human cervical carcinoma cell line (Hela)

Nitric Oxide ◽  
2007 ◽  
Vol 17 ◽  
pp. 21
Author(s):  
D. Dong Jin ◽  
T. Yaping ◽  
F. Zhengyu
Keyword(s):  
Cell Line ◽  
Cervical Carcinoma ◽  
Carcinoma Cell ◽  
Carcinoma Cell Line ◽  
Human Cervical Carcinoma ◽  
Cervical Carcinoma Cell Line ◽  
Cell Line Hela ◽  
Line Hela

scholarly journals FOLIC ACID-CONJUGATED DOXORUBICIN-LOADED PHOTOSENSITIZING MANGANESE FERRITE NANOPARTICLES: SYNTHESIS, CHARACTERIZATION AND ANTICANCER ACTIVITY AGAINST HUMAN CERVICAL CARCINOMA CELL LINE (HELA)

2017 ◽  
Vol 9 (5) ◽  
pp. 60
Author(s):  
Muhammad Hassan Safdar ◽  
Humna Hasan ◽  
Mariam Anees ◽  
Zahid Hussain
Keyword(s):  
Cell Line ◽  
Cervical Carcinoma ◽  
Carcinoma Cell ◽  
Ferrite Nanoparticles ◽  
Carcinoma Cell Line ◽  
Manganese Ferrite ◽  
Human Cervical Carcinoma ◽  
Cervical Carcinoma Cell Line ◽  
Cell Line Hela ◽  
Line Hela

Objective: On account of several complications and adverse effects associated with the use of conventional chemotherapeutic regimen, the advanced drug-targeted therapies have gained the remarkable attention of the researchers due to their fabulous pharmaceutical and therapeutic advantages. The present study was designed with the aim to synthesize manganese ferrite nanoparticles (MnFe2O4 NPs) and folic acid-conjugated doxorubicin (DOX)-loaded manganese ferrite bovine serum albumin NPs (FA-BSA-DOX-MnFe2O4 NPs) using desolvation cross-linking method.Methods: Having assessed their physicochemical characteristics, the prepared NPs were evaluated for hem compatibility, photo-mediated cytotoxicity, and anti-cancer potential against human cervical carcinoma cell line (HeLa) using a range of in vitro assays which include hemolysis assay, sulforhodamine B (SRB) and MTT assays.Results: Spectroscopic characterization revealed that MnFe2O4 NPs were spherical with an average size diameter of approx. 15 nm and a band gap of 1.4 eV. Another remarkable feature of FA-BSA-DOX conjugated MnFe2O4 NPs was high entrapment efficiency (approx. 95%). MTT assay demonstrated that MnFe2O4 NPs revealed potential photosensitizing ability upon exposure to sunlight. FA-BSA-DOX conjugated MnFe2O4 NPs showed promising cytotoxicity against human cervical epithelial malignant carcinoma cell line (HeLa). Interestingly, the cytotoxicity of these NPs was gradually increased with time of exposure to sunlight.Conclusion: These findings suggested that FA-BSA-DOX conjugated MnFe2O4NPs exhibit promising photosensitizing and anticancer potential against human cervical carcinoma and thus can be considered as an alternative targeted therapy against human cervical cancer. 

scholarly journals SYNTHESIS, IDENTIFICATION AND IN VITRO ANTITUMOUR PRESCREENING TEST OF TRIPHENYLTIN BENZOATE TOWARDS A HUMAN CERVICAL CARCINOMA CELL LINE, HeLa

2010 ◽  
Vol 8 (3) ◽  
pp. 418-422
Author(s):  
Ida Bagus Putra Manuaba
Keyword(s):  
Cell Line ◽  
Cervical Carcinoma ◽  
Carcinoma Cell ◽  
Human Cervical Carcinoma ◽  
Cervical Carcinoma Cell Line ◽  
Redistribution Reaction ◽  
Cell Line Hela ◽  
Triphenyltin Chloride ◽  
Line Hela

In this study, triphenyltin benzoate was synthesized first, and followed by antitumor prescreening test of the compound towards a human cervical carcinoma cell line, HeLa. Three reaction steps were employed to obtain the compound needed, i.e. 1) synthesizing of tetraphenyltin compound via insitu phenilmagnesiumbromide Grignard reaction to tin(IV)chloride, 2) synthesizing triphenyltin chloride via redistribution reaction of tetraphenyltin to tin(IV) chloride without any solvent, the reaction completed depends on the temperature, in this case a good results was achieved at temperature 220 °C for 6 h, 3) finally, triphenyltin benzoate was produced through a methathetical reaction of triphenyltin chloride to an excess of sodium benzoate in ethanol. In vitro prescreening antitumour activity of the compound towards a human cervical tumour cell line, HeLa was carried out following an enzyme linked immunosorbent assays (ELISA). By this method, the test ended with good promising results. This indicates by the IC50 of 170 nM which is compared well to cisplatinum with IC50 950 nM.   Keywords: redistribution reaction, methathetical reaction, cell line, in vitro, antitumour

Abstract B208: Reduction in P2X7 receptor expression is a marker of resistance to ATP treatment in human cervical carcinoma cell line.

2013 ◽  
Author(s):  
Paola de Andrade Mello ◽  
Eduardo Cremonese Filippi-Chiela ◽  
Jessica Nascimento ◽  
Franciele Cristina Kipper ◽  
Aline Beckenkamp ◽  
...  
Keyword(s):  
Cell Line ◽  
Cervical Carcinoma ◽  
P2x7 Receptor ◽  
Carcinoma Cell ◽  
Receptor Expression ◽  
Carcinoma Cell Line ◽  
Human Cervical Carcinoma ◽  
Cervical Carcinoma Cell Line

scholarly journals Identification of a cancer stem cell-like side population in the HeLa human cervical carcinoma cell line

2013 ◽  
Vol 6 (6) ◽  
pp. 1673-1680 ◽  
Author(s):  
KEFANG WANG ◽  
JIANFANG ZENG ◽  
LIJING LUO ◽  
JIAXIN YANG ◽  
JIE CHEN ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cancer Stem Cell ◽  
Cell Line ◽  
Cervical Carcinoma ◽  
Carcinoma Cell ◽  
Side Population ◽  
Carcinoma Cell Line ◽  
Human Cervical Carcinoma ◽  
Cervical Carcinoma Cell Line

scholarly journals Glucocorticoids enhance cytotoxicity of cisplatin via suppression of NF-κB activation in the glucocorticoid receptor-rich human cervical carcinoma cell line SiHa

2006 ◽  
Vol 188 (2) ◽  
pp. 311-319 ◽  
Author(s):  
Yen-Shen Lu ◽  
Pei-Yen Yeh ◽  
Shuang-En Chuang ◽  
Ming Gao ◽  
Min-Liang Kuo ◽  
...  
Keyword(s):  
Cell Line ◽  
Cervical Carcinoma ◽  
Carcinoma Cell ◽  
Carcinoma Cells ◽  
Dominant Negative ◽  
Carcinoma Cell Line ◽  
Drug Induced ◽  
Enhancing Effect ◽  
Human Cervical Carcinoma ◽  
Cervical Carcinoma Cell Line

Glucocorticoids (GCs) are commonly co-administered with cisplatin in the treatment of patients with carcinomas to prevent drug-induced allergic reaction, nausea and vomiting. Although GC receptor (GR) is ubiquitous in carcinoma cells and has been linked to signal transduction pathways pertinent to cell growth and apoptosis, little is known regarding the possible effect of GC on the chemosensitivity of carcinomas. Our previous study demonstrated that dexamethasone (DEX) enhances the cytotoxicity to cisplatin in a GR-rich human cervical carcinoma cell line, SiHa. In this study, we found that this cisplatin cytotoxicity-enhancing effect of DEX correlated well with its effect on abrogating the cisplatin-induced activation of nuclear factor kappa B (NF-κB). RU486, a structural homologue of DEX, partially reversed this cytotoxicity-enhancing effect of DEX, a finding consistent with the well-known partial reversing effect of RU486 on DEX-induced NF-κB suppression. Furthermore, expression of a dominant-negative truncated IκBα gene in SiHa cells completely abolished the cisplatin cytotoxicity-enhancing effect of DEX. Our data suggest that the specific action of DEX on GR may enhance the cytotoxicity of cisplatin in selected GR-rich cancer cells by suppressing NF-κB activation.

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