Demineralized Bone Matrix and Fat Autograft in a Rabbit Model of Frontal Sinus Obliteration

2007 ◽  
Vol 137 (2) ◽  
pp. 264-268 ◽  
Author(s):  
Jason I. Altman ◽  
Jean Anderson Eloy ◽  
Benjamin L. Hoch ◽  
Carla M. Munoz ◽  
Michael R. Shohet
Keyword(s):  
Frontal Sinus ◽  
Demineralized Bone Matrix ◽  
Bone Matrix ◽  
Rabbit Model ◽  
Spiral Ct ◽  
Radiologic Evaluation ◽  
Sinus Cavity ◽  
Materials Used ◽  
Sinus Obliteration ◽  
Demineralized Bone

OBJECTIVE: In this study, we investigate the efficacy of demineralized bone matrix (DBM) as a material for frontal sinus obliteration in a rabbit model. STUDY DESIGN AND SETTING: Twenty-four New Zealand White rabbits were divided into four groups, and the study was carried out to two time periods. Twelve rabbits underwent frontal sinus obliteration with fat autograft, and 12 rabbits underwent the procedure with DBM. At 12 weeks, six control and six study rabbits were killed. The remaining 12 rabbits were killed at 36 weeks. All specimens underwent radiologic evaluation with spiral CT followed by histologic examination for evidence of bony growth. RESULTS: Sinuses obliterated with DBM showed replacement of the sinus cavity by trabecular bone. Histology demonstrated significant progressive replacement of DBM by cancellous bone from 12 weeks (53.3%) to 36 weeks (78.8%). There were no complications observed as a result of the materials used. CONCLUSION AND SIGNIFICANCE: DBM is a prospective material for frontal sinus obliteration. Long-term studies and human trials will further elucidate the role of this material.

R017: Demineralized Bone Matrix for Frontal Sinus Obliteration

2006 ◽  
Vol 135 (2_suppl) ◽  
pp. P110-P111
Author(s):  
Jean Anderson Eloy ◽  
Jason I Altman ◽  
Carla M Munoz ◽  
Michael Robert Shohet
Keyword(s):  
Frontal Sinus ◽  
Demineralized Bone Matrix ◽  
Bone Matrix ◽  
Sinus Obliteration ◽  
Demineralized Bone

scholarly journals Histopathological and Radiographical Evaluation of Caprine Demineralized Bone Matrix in a Critical Ulnar Defect in a Rabbit Model

2021 ◽  
Author(s):  
Olawale Alimi Alimi ◽  
Adamu Abdul Abuabakar ◽  
Abubakar Sadiq Yakubu ◽  
Sani Abdullahi Shehu ◽  
Salman Zubairu Abdulkadir
Keyword(s):  
Bone Defect ◽  
Demineralized Bone Matrix ◽  
Bone Matrix ◽  
Rabbit Model ◽  
Negative Control ◽  
Autogenous Bone ◽  
Level Of Evidence ◽  
Defect Sites ◽  
Significant Difference ◽  
Demineralized Bone

Abstract Background: Caprine species satisfy the conditions of an ideal donor animal when compared to bovine species that has been extensively studied and commercialized for bone xenograft. Histopathological and radiological evaluations of caprine demineralized bone matrix (CDBM) were therefore carried out for fracture healing properties for its possible use in bone grafting procedures. Materials and Methods: Twenty-four rabbits were used for this study and were divided randomly into three groups of eight (n=8) rabbits each. Critical bone defect was created on the ulnar diaphysis under xylazine-ketamine anaesthesia for autogenous bone graft (ABG) group, CDBM group and the last group was left unfilled as negative control (NC). Immediate post-grafting radiograph was taken and repeated on days 14, 28, 42 and 56 to monitor the evidence of radiographic healing. The animals were euthanized on day 56 and defect sites were harvested for histopathology. Results: There was a progressive evidence of radiographic healing and bone formation in all the groups with significance difference (P=0.0064). When compared with ABG, NC differ significantly (P<0.0001) whereas the CDBM did not differ significantly (P=0.6765). The histopathology sections of ABG and CDBM showed normal bone tissue while the NC section was predominated by fibrous connective tissue. There was therefore an overall significant difference (P=0.0001) in which CDBM did not differ from ABG (P=0.2946) while NC did (P=0.0005). Conclusion: The ABG and CDBM groups showed a similar healing effect in the critical bone defect. Therefore, CDBM could be used as an effective alternative to ABG in orthopaedics to circumvent the limitations and complications associated with it. Level of Evidence: Not applicable.

Autograft versus allograft with or without demineralized bone matrix in posterolateral lumbar fusion in rabbits

2008 ◽  
Vol 9 (1) ◽  
pp. 84-89 ◽  
Author(s):  
Julio Urrutia ◽  
Nicolas Thumm ◽  
Daniel Apablaza ◽  
Felipe Pizarro ◽  
Alejandro Zylberberg ◽  
...  
Keyword(s):  
Demineralized Bone Matrix ◽  
Lumbar Fusion ◽  
Donor Site ◽  
Bone Matrix ◽  
Rabbit Model ◽  
Fusion Rate ◽  
Iliac Crest Bone Graft ◽  
Fisher Exact Test ◽  
Group I ◽  
Demineralized Bone

Object Posterolateral spinal fusions are performed to treat different spinal disorders. Autograft continues to be the gold standard; it is, however, associated with donor site morbidity and limited sources. Allograft has been used, but has been reported to result in lower fusion rates. Demineralized bone matrix (DBM) has also been used and reportedly increases the fusion rate in a variety of critical defect models. Different forms of DBM are available, not all have been independently studied. To evaluate the effect of a xenogenic DBM added to allograft on the fusion rate of posterolateral lumbar spine arthrodesis the authors designed an experimental study comparing posterolateral fusion rate using autograft, allograft, and allograft plus a xenogenic DBM in a validated animal model. Methods A bilateral, 1-level (L4–5) intertransverse process fusion was performed in 45 male New Zealand rabbits. Iliac crest bone graft was harvested bilaterally from each rabbit. The rabbits were randomly assigned to 3 groups: Group I, Autograft, 15 rabbits; Group II, Allograft, 15 rabbits; and Group III, Allograft plus DBM in a paste form (Dynagraft). The animals were killed 8 weeks after surgery. Fusion was assessed radiographically and by manual palpation by 2 independent observers. The results were analyzed using the Fisher exact test and chi-square test. Results The fusion rate was 46.6% (7 of 15 rabbits) in the autograft group, 33.3% (5 of 15 rabbits) in the allograft group, and 33.3% (5 of 15 rabbits) in the allograft plus DBM group (p > 0.05). Conclusions Autograft produced a higher fusion rate than allograft in this spinal fusion rabbit model, but the difference was not statistically significant. Allograft plus xenogenic DBM showed the same fusion rate as allograft alone.

scholarly journals Does demineralized bone matrix enhance tendon-to-bone healing after rotator cuff repair in a rabbit model?

2019 ◽  
Author(s):  
Woo-Yong Lee ◽  
Young-Mo Kim ◽  
Hyun-Dae Shin ◽  
Deuk-Soo Hwang ◽  
Yong-Bum Joo ◽  
...  
Keyword(s):  
Rotator Cuff ◽  
Demineralized Bone Matrix ◽  
Bone Matrix ◽  
Rabbit Model ◽  
Longitudinal Direction ◽  
Collagen Fibers ◽  
Control Group ◽  
The Right ◽  
Demineralized Bone

Abstract Background The purpose of this study was to compare the histologic outcomes after rotator cuff (RC) repair between with demineralized bone matrix (DBM) augmentation and without DBM and to evaluate the role of DBM for tendon-to-bone (TB) healing in a rabbit model. Methods Twenty-six adult male New Zealand white rabbits were randomly allocated to the control group (n = 13) or the DBM group (n = 13). A chronic RC tear was generated on the right shoulder of all rabbits. In the control group, RC repair was achieved by a standard transosseous technique. In the DBM group, RC repair was achieved using the same technique, and DBM was interposed between the cuff and bone. After 8 weeks, the RC tendon entheses from all rabbits were processed for gross and histologic examination. Results In the control group, the tendon midsubstance was disorganized with randomly and loosely arranged collagen fibers and rounded fibroblastic nuclei. The TB interface was predominantly fibrous with small regions of fibrocartilage, especially mineralized fibrocartilage. In the DBM group, the tendon midsubstance appeared normal and comprised densely arranged collagen fibers, with orientated crimped collagen fibers running in the longitudinal direction of the tendon. These fibers were interspersed with elongated fibroblast nuclei. The TB interface consisted of organized collagen fibers with large quantities of fibrocartilage and mineralized fibrocartilage. Conclusion DBM augmentation at the RC-to-bone interface enhances TB healing after RC repair.

The Extraction Processes and Biological Characteristics of a New Generation Demineralized Bone Matrixes

2011 ◽  
Vol 217-218 ◽  
pp. 1006-1013
Author(s):  
Zhi Yu Zhou ◽  
Li Jin Zou ◽  
Hai Sheng Li ◽  
Cody Bunger ◽  
Xue Nong Zou
Keyword(s):  
Bone Graft ◽  
Demineralized Bone Matrix ◽  
Bone Matrix ◽  
Biological Characteristics ◽  
Graft Material ◽  
Bone Graft Material ◽  
Extraction Processes ◽  
Materials Used ◽  
New Generation ◽  
Demineralized Bone

An ideal bone graft material should have osteocondutive, osteroindurctive, and osteogenic features. Scientists and doctors have been trying to develop this kind of material for over one century. However, all the features of few materials used in clinic now have been qualified. Recently, COLLOSS, as the new generation bone graft material of demineralized bone matrix, almost achieved this height. The paper presents a general survey of COLLOSS including its extraction processes, biological characteristics, and application prospects.

scholarly journals Does Demineralized Bone Matrix Enhance Tendon-to-Bone Healing after Rotator Cuff Repair in a Rabbit Model?

2021 ◽  
Vol 13 ◽  
Author(s):  
Woo-Yong Lee ◽  
Young-Mo Kim ◽  
Deuk-Soo Hwang ◽  
Hyun-Dae Shin ◽  
Yong-Bum Joo ◽  
...  
Keyword(s):  
Rotator Cuff ◽  
Bone Healing ◽  
Rotator Cuff Repair ◽  
Demineralized Bone Matrix ◽  
Bone Matrix ◽  
Rabbit Model ◽  
Cuff Repair ◽  
Demineralized Bone
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