Autograft versus allograft with or without demineralized bone matrix in posterolateral lumbar fusion in rabbits

2008 ◽  
Vol 9 (1) ◽  
pp. 84-89 ◽  
Author(s):  
Julio Urrutia ◽  
Nicolas Thumm ◽  
Daniel Apablaza ◽  
Felipe Pizarro ◽  
Alejandro Zylberberg ◽  
...  
Keyword(s):  
Demineralized Bone Matrix ◽  
Lumbar Fusion ◽  
Donor Site ◽  
Bone Matrix ◽  
Rabbit Model ◽  
Fusion Rate ◽  
Iliac Crest Bone Graft ◽  
Fisher Exact Test ◽  
Group I ◽  
Demineralized Bone

Object Posterolateral spinal fusions are performed to treat different spinal disorders. Autograft continues to be the gold standard; it is, however, associated with donor site morbidity and limited sources. Allograft has been used, but has been reported to result in lower fusion rates. Demineralized bone matrix (DBM) has also been used and reportedly increases the fusion rate in a variety of critical defect models. Different forms of DBM are available, not all have been independently studied. To evaluate the effect of a xenogenic DBM added to allograft on the fusion rate of posterolateral lumbar spine arthrodesis the authors designed an experimental study comparing posterolateral fusion rate using autograft, allograft, and allograft plus a xenogenic DBM in a validated animal model. Methods A bilateral, 1-level (L4–5) intertransverse process fusion was performed in 45 male New Zealand rabbits. Iliac crest bone graft was harvested bilaterally from each rabbit. The rabbits were randomly assigned to 3 groups: Group I, Autograft, 15 rabbits; Group II, Allograft, 15 rabbits; and Group III, Allograft plus DBM in a paste form (Dynagraft). The animals were killed 8 weeks after surgery. Fusion was assessed radiographically and by manual palpation by 2 independent observers. The results were analyzed using the Fisher exact test and chi-square test. Results The fusion rate was 46.6% (7 of 15 rabbits) in the autograft group, 33.3% (5 of 15 rabbits) in the allograft group, and 33.3% (5 of 15 rabbits) in the allograft plus DBM group (p > 0.05). Conclusions Autograft produced a higher fusion rate than allograft in this spinal fusion rabbit model, but the difference was not statistically significant. Allograft plus xenogenic DBM showed the same fusion rate as allograft alone.

scholarly journals Comparison of Fusion Rate between Demineralized Bone Matrix versus Autograft in Lumbar Fusion : Meta-Analysis

2020 ◽  
Vol 63 (6) ◽  
pp. 673-680 ◽  
Author(s):  
Sanghyun Han ◽  
Bumsoo Park ◽  
Jeong-Wook Lim ◽  
Jin-Young Youm ◽  
Seoung-Won Choi ◽  
...  
Keyword(s):  
Demineralized Bone Matrix ◽  
Lumbar Fusion ◽  
Meta Analysis ◽  
Bone Matrix ◽  
Fusion Rate ◽  
Matrix Versus ◽  
Demineralized Bone

Comparative Assessment of Autogenous Cancellous Bone Graft and Bovine-Derived Demineralized Bone Matrix for Secondary Alveolar Bone Grafting in Patients With Unilateral Cleft Lip and Palate

2021 ◽  
pp. 105566562110251
Author(s):  
Vijay Kumar ◽  
Vidya Rattan ◽  
Sachin Rai ◽  
Satinder Pal Singh ◽  
Jai Kumar Mahajan
Keyword(s):  
Bone Graft ◽  
Iliac Crest ◽  
Demineralized Bone Matrix ◽  
Bone Matrix ◽  
Volumetric Analysis ◽  
Group I ◽  
Alveolar Bone Grafting ◽  
Group Ii ◽  
Demineralized Bone

Objective: Comparison between bovine-derived demineralized bone matrix (DMBM) and iliac crest graft over long term for secondary alveolar bone grafting (SABG) in patients with unilateral cleft lip and palate (UCLP) in terms of radiological and clinical outcomes. Design: Prospective, randomized, parallel groups, double-blind, controlled trial. Setting: Unit of Oral and Maxillofacial Surgery, Oral Health Science Centre, Postgraduate Institute of Medical Education & Research, Chandigarh. Participants: Twenty patients with UCLP. Interventions: Patients were allocated into group I (Iliac crest bone graft) and group II (DMBM) for SABG. Outcomes were assessed at 2 weeks, 6 months, and then after mean follow-up period of 63 months. Outcomes Measures: Volumetric analysis of the grafted bone in the alveolar cleft site was done through cone beam computed tomography using Cavalieri principle and modified assessment tool. Clinical assessment was performed in terms of pain, swelling, duration of hospital stay, cost of surgery, alar base symmetry, and donor site morbidity associated with iliac crest harvesting. Results: Volumetric analysis through Cavalieri principle revealed comparable bone uptake at follow-up of 6 months between group I (70%) and group II (69%). Modified assessment tool showed no significant difference between horizontal and vertical bone scores over short- and long-term follow-up. In group II, there was higher cost of surgery, but no donor site morbidity unlike group I. Conclusions: Demineralized bone matrix proved analogous to iliac crest bone graft as per volumetric analysis over shorter period. However, although statistically insignificant, net bone volume achieved was lower than the iliac crest graft at longer follow-up.

scholarly journals Histopathological and Radiographical Evaluation of Caprine Demineralized Bone Matrix in a Critical Ulnar Defect in a Rabbit Model

2021 ◽  
Author(s):  
Olawale Alimi Alimi ◽  
Adamu Abdul Abuabakar ◽  
Abubakar Sadiq Yakubu ◽  
Sani Abdullahi Shehu ◽  
Salman Zubairu Abdulkadir
Keyword(s):  
Bone Defect ◽  
Demineralized Bone Matrix ◽  
Bone Matrix ◽  
Rabbit Model ◽  
Negative Control ◽  
Autogenous Bone ◽  
Level Of Evidence ◽  
Defect Sites ◽  
Significant Difference ◽  
Demineralized Bone

Abstract Background: Caprine species satisfy the conditions of an ideal donor animal when compared to bovine species that has been extensively studied and commercialized for bone xenograft. Histopathological and radiological evaluations of caprine demineralized bone matrix (CDBM) were therefore carried out for fracture healing properties for its possible use in bone grafting procedures. Materials and Methods: Twenty-four rabbits were used for this study and were divided randomly into three groups of eight (n=8) rabbits each. Critical bone defect was created on the ulnar diaphysis under xylazine-ketamine anaesthesia for autogenous bone graft (ABG) group, CDBM group and the last group was left unfilled as negative control (NC). Immediate post-grafting radiograph was taken and repeated on days 14, 28, 42 and 56 to monitor the evidence of radiographic healing. The animals were euthanized on day 56 and defect sites were harvested for histopathology. Results: There was a progressive evidence of radiographic healing and bone formation in all the groups with significance difference (P=0.0064). When compared with ABG, NC differ significantly (P<0.0001) whereas the CDBM did not differ significantly (P=0.6765). The histopathology sections of ABG and CDBM showed normal bone tissue while the NC section was predominated by fibrous connective tissue. There was therefore an overall significant difference (P=0.0001) in which CDBM did not differ from ABG (P=0.2946) while NC did (P=0.0005). Conclusion: The ABG and CDBM groups showed a similar healing effect in the critical bone defect. Therefore, CDBM could be used as an effective alternative to ABG in orthopaedics to circumvent the limitations and complications associated with it. Level of Evidence: Not applicable.

Complications Following Arthroscopic Ankle Arthrodesis

1996 ◽  
Vol 17 (6) ◽  
pp. 340-342 ◽  
Author(s):  
Lynn A. Crosby ◽  
Ted C. Yee ◽  
Teri S. Formanek ◽  
Timothy C. Fitzgibbons
Keyword(s):  
Bone Marrow ◽  
Complication Rate ◽  
Demineralized Bone Matrix ◽  
Bone Matrix ◽  
Fusion Rate ◽  
Ankle Arthrodesis ◽  
Deep Infection ◽  
Pin Site ◽  
Demineralized Bone

Forty-two patients underwent an arthroscopic ankle arthrodesis utilizing a bi-framed distraction technique and demineralized bone matrix-bone marrow slurry as a graft substitute. The average follow-up was 27 months (range, 12–64 months). The overall complication rate was 55%, including three nonunions (7%), two fractures (4.8%), four pin site infections (9.5%), one deep infection, four hardware problems (9.5%), and four symptomatic painful subtalar joints (9.5%). Overall, 85% of patients were satisfied with their final result. The complication rate was high but most complications were minor and manageable. The demineralized bone matrix and bone marrow did not seem to increase the fusion rate over what has been documented previously for arthroscopic ankle fusions without the use of this graft substitute.

scholarly journals Allograft Versus Demineralized Bone Matrix in Instrumented and Noninstrumented Lumbar Fusion: A Systematic Review

2017 ◽  
Vol 8 (4) ◽  
pp. 396-412 ◽  
Author(s):  
Zorica Buser ◽  
Darrel S. Brodke ◽  
Jim A. Youssef ◽  
Elke Rometsch ◽  
Jong-Beom Park ◽  
...  
Keyword(s):  
Systematic Review ◽  
Demineralized Bone Matrix ◽  
Lumbar Fusion ◽  
Bone Matrix ◽  
Demineralized Bone

scholarly journals Does demineralized bone matrix enhance tendon-to-bone healing after rotator cuff repair in a rabbit model?

2019 ◽  
Author(s):  
Woo-Yong Lee ◽  
Young-Mo Kim ◽  
Hyun-Dae Shin ◽  
Deuk-Soo Hwang ◽  
Yong-Bum Joo ◽  
...  
Keyword(s):  
Rotator Cuff ◽  
Demineralized Bone Matrix ◽  
Bone Matrix ◽  
Rabbit Model ◽  
Longitudinal Direction ◽  
Collagen Fibers ◽  
Control Group ◽  
The Right ◽  
Demineralized Bone

Abstract Background The purpose of this study was to compare the histologic outcomes after rotator cuff (RC) repair between with demineralized bone matrix (DBM) augmentation and without DBM and to evaluate the role of DBM for tendon-to-bone (TB) healing in a rabbit model. Methods Twenty-six adult male New Zealand white rabbits were randomly allocated to the control group (n = 13) or the DBM group (n = 13). A chronic RC tear was generated on the right shoulder of all rabbits. In the control group, RC repair was achieved by a standard transosseous technique. In the DBM group, RC repair was achieved using the same technique, and DBM was interposed between the cuff and bone. After 8 weeks, the RC tendon entheses from all rabbits were processed for gross and histologic examination. Results In the control group, the tendon midsubstance was disorganized with randomly and loosely arranged collagen fibers and rounded fibroblastic nuclei. The TB interface was predominantly fibrous with small regions of fibrocartilage, especially mineralized fibrocartilage. In the DBM group, the tendon midsubstance appeared normal and comprised densely arranged collagen fibers, with orientated crimped collagen fibers running in the longitudinal direction of the tendon. These fibers were interspersed with elongated fibroblast nuclei. The TB interface consisted of organized collagen fibers with large quantities of fibrocartilage and mineralized fibrocartilage. Conclusion DBM augmentation at the RC-to-bone interface enhances TB healing after RC repair.

scholarly journals Efficacy, Cost, and Complications of Demineralized Bone Matrix in Instrumented Lumbar Fusion: Comparison With rhBMP-2

2020 ◽  
pp. 219256822094250
Author(s):  
Ananth Eleswarapu ◽  
F. Andrew Rowan ◽  
Hai Le ◽  
Joseph B. Wick ◽  
Rolando F. Roberto ◽  
...  
Keyword(s):  
Clinical Outcomes ◽  
Interbody Fusion ◽  
Demineralized Bone Matrix ◽  
Short Form ◽  
Lumbar Fusion ◽  
Bone Matrix ◽  
Final Analysis ◽  
Transforaminal Lumbar Interbody Fusion ◽  
Bone Morphogenetic Protein 2 ◽  
Demineralized Bone

Study Design: Retrospective cohort study. Objectives: To evaluate demineralized bone matrix as an adjunct for instrumented lumbar spine fusion compared with recombinant human bone morphogenetic protein–2 (rhBMP-2). Methods: Clinical and radiographic review was performed of 43 patients with degenerative spine disease treated with posterolateral spinal fusion with or without posterior or transforaminal lumbar interbody fusion. Final analysis included sixteen patients treated with demineralized bone matrix (DBM; Accell Evo3, SeaSpine) compared with a retrospective matched group of 21 patients treated with rhBMP-2 (rhBMP-2, Infuse, Medtronic). All patients were followed for 24 months. Fusion was evaluated by computed tomography and/or x-ray. Clinical outcomes included visual analogue scale (VAS), Oswestry Disability Index (ODI), and Short Form 12 (SF-12). Results: Overall fusion rate, including posterolateral and/or interbody fusion, was 100% for both groups, though the fusion rates in the posterolateral space alone were 93.5% and 100% for the DBM and rhBMP-2 groups, respectively. Clinical outcomes were similar between groups, with the DBM group showing greater improvement in ODI. The rhBMP-2 group showed higher rates of radiographic complications with 7 of 21 patients (33.3%) demonstrating either adjacent level fusion or ectopic bone formation, compared with zero in the DBM group. Average biologic cost per level was $1522 for DBM and $3505 for rhBMP-2. Conclusions: DBM and rhBMP-2 demonstrated similar radiographic and clinical outcomes in instrumented lumbar fusions. rhBMP-2 was associated with higher rates of radiographic complications and significantly higher costs.

Demineralized Bone Matrix and Fat Autograft in a Rabbit Model of Frontal Sinus Obliteration

2007 ◽  
Vol 137 (2) ◽  
pp. 264-268 ◽  
Author(s):  
Jason I. Altman ◽  
Jean Anderson Eloy ◽  
Benjamin L. Hoch ◽  
Carla M. Munoz ◽  
Michael R. Shohet
Keyword(s):  
Frontal Sinus ◽  
Demineralized Bone Matrix ◽  
Bone Matrix ◽  
Rabbit Model ◽  
Spiral Ct ◽  
Radiologic Evaluation ◽  
Sinus Cavity ◽  
Materials Used ◽  
Sinus Obliteration ◽  
Demineralized Bone

OBJECTIVE: In this study, we investigate the efficacy of demineralized bone matrix (DBM) as a material for frontal sinus obliteration in a rabbit model. STUDY DESIGN AND SETTING: Twenty-four New Zealand White rabbits were divided into four groups, and the study was carried out to two time periods. Twelve rabbits underwent frontal sinus obliteration with fat autograft, and 12 rabbits underwent the procedure with DBM. At 12 weeks, six control and six study rabbits were killed. The remaining 12 rabbits were killed at 36 weeks. All specimens underwent radiologic evaluation with spiral CT followed by histologic examination for evidence of bony growth. RESULTS: Sinuses obliterated with DBM showed replacement of the sinus cavity by trabecular bone. Histology demonstrated significant progressive replacement of DBM by cancellous bone from 12 weeks (53.3%) to 36 weeks (78.8%). There were no complications observed as a result of the materials used. CONCLUSION AND SIGNIFICANCE: DBM is a prospective material for frontal sinus obliteration. Long-term studies and human trials will further elucidate the role of this material.

A Historical Review of Common Bone Graft Materials in Foot and Ankle Surgery

2014 ◽  
Vol 7 (2) ◽  
pp. 143-151 ◽  
Author(s):  
Justin W. Arner ◽  
Robert D. Santrock
Keyword(s):  
Bone Graft ◽  
Demineralized Bone Matrix ◽  
Donor Site ◽  
Bone Matrix ◽  
Historical Review ◽  
Donor Site Morbidity ◽  
Iliac Crest Bone Graft ◽  
Foot And Ankle ◽  
Ankle Surgery ◽  
Bone Graft Substitutes

Foot and ankle fusion is an important treatment for arthritis and deformities of the ankle and hindfoot. The literature has shown that there are improvements in fusion rates with the addition of bone graft and bone graft substitutes. Today autografts, specifically the iliac crest bone graft (ICBG), continue to be the gold standard despite significant donor site morbidity and nonunion rates, persisting around 10%. To address these drawbacks, bone graft substitutes have been developed. This article includes a historical review of the use, outcomes, and safety of autografts, allografts, and bone graft substitutes, such as ceramics, demineralized bone matrix, and platelet-derived growth factor.

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