Sequence and phylogenetic analysis of the thrombospondin-related adhesive protein gene of Babesia gibsoni isolates in dogs in South India

2022 ◽  
Vol 86 ◽  
pp. 102477
Author(s):  
Christophe Angeline Felicia Bora ◽  
Anju Varghese ◽  
Chundayil Kalarickal Deepa ◽  
Ashwathappa Nandini ◽  
Lanchalung Malangmei ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
South India ◽  
Protein Gene ◽  
Babesia Gibsoni ◽  
Adhesive Protein

scholarly journals Sequence and Phylogenetic Analysis of the Thrombospondin-Related Adhesive Protein (TRAP) Gene of Babesia gibsoni Isolates from Dogs in Taiwan

2010 ◽  
Vol 72 (10) ◽  
pp. 1329-1335 ◽  
Author(s):  
Chung-Chan LEE ◽  
Yu-Chen HSIEH ◽  
Chia-Chia HUANG ◽  
Chau-Loong TSANG ◽  
Yang-Tsung CHUNG
Keyword(s):  
Phylogenetic Analysis ◽  
Babesia Gibsoni ◽  
Adhesive Protein ◽  
Trap Gene

Avipoxvirus detected in tumor-like lesions in a white-faced whistling duck (Dendrocygna viduata)

2020 ◽  
Vol 40 (10) ◽  
pp. 818-823
Author(s):  
Juliana F.V. Braga ◽  
Rodrigo M. Couto ◽  
Marcelo C. Rodrigues ◽  
Roselene Ecco
Keyword(s):  
Squamous Cell Carcinoma ◽  
Phylogenetic Analysis ◽  
Protein Gene ◽  
Core Protein ◽  
Avian Species ◽  
Tissue Samples ◽  
Histopathological Evaluation ◽  
In Captivity ◽  
Avian Pox ◽  
Tumor Like Lesions

ABSTRACT: Avipoxvirus is the etiological agent of the avian pox, a well-known disease of captive and wild birds, and it has been associated with tumor-like lesions in some avian species. A white-faced whistling duck (Dendrocygna viduata) raised in captivity was referred to a Veterinary Teaching Hospital in Northeast due to cutaneous nodules present in both wings. A few days after the clinical examination, the animal died naturally. Once submitted to necropsy, histopathological evaluation of the lesions revealed clusters of proliferating epithelial cells expanding toward the dermis. Some of these cells had round, well-defined, intracytoplasmic eosinophilic material suggestive of poxvirus inclusion (Bollinger bodies). PCR performed on the DNA extracted from tissue samples amplified a fragment of the 4b core protein gene (fpv167), which was purified and sequenced. This fragment of Avipoxvirus DNA present in these tumor-like lesions showed high genetic homology (100.0%) with other poxviruses detected in different avian species in several countries, but none of them were related to tumor-like lesions or squamous cell carcinoma. This is the first report of Avipoxvirus detected in tumor-like lesions of a white-faced whistling duck with phylogenetic analysis of the virus.

Cloning and expression of the thrombospondin related adhesive protein gene of Plasmodium berghei1Note: GenBank submission number: U677631

1997 ◽  
Vol 84 (1) ◽  
pp. 1-12 ◽  
Author(s):  
Kathryn J.H Robson ◽  
Silvia Naitza ◽  
Guy Barker ◽  
Robert E Sinden ◽  
Andrea Crisanti
Keyword(s):  
Protein Gene ◽  
Cloning And Expression ◽  
Adhesive Protein

scholarly journals Detection of Babesia canis vogeli, Babesia gibsoni and Ehrlichia canis by multiplex PCR in naturally infected dogs in South India

2018 ◽  
Vol 88 (2) ◽  
pp. 215-224 ◽  
Author(s):  
Jose Jain ◽  
◽  
Bindu Lakshmanan ◽  
Hitaishi V. Nagaraj ◽  
Jose E. Praveena ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
South India ◽  
Ehrlichia Canis ◽  
Babesia Canis ◽  
Babesia Gibsoni

scholarly journals Genomic Analysis and Comparison of Pakistani Camels (Camelus dromedarius) by Prion Gene

2017 ◽  
Vol 45 (1) ◽  
pp. 8
Author(s):  
Tanveer Hussain ◽  
Muneeb Musthafa ◽  
Masroor Ellahi Babar ◽  
Faiz Marikar ◽  
Fiaz Hussain ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Prion Protein ◽  
Neurodegenerative Disorders ◽  
Genomic Dna ◽  
Protein Gene ◽  
Camelus Dromedarius ◽  
Prion Protein Gene ◽  
Dromedary Camels ◽  
Prion Gene ◽  
Bactrian Camels

Background: In many parts of the Old World, domesticated camels (genus - Camelus) are an essential resource, providing food, labor, commodities, and sport to millions of people Of the three extent species, two have been domesticated (singlehumped dromedarius, Camelus dromedarius, and two humped Bactrian camels Camelus bactrianus) and one remains wild (two-humped wild Bactrian camels Camelus ferus). All three species possess a variety adaptations to harsh desert conditions, including mechanisms to tolerate of extreme temperatures, dehydration, and sandy terrain. People residing in harsh climate zones of the world are being benefitted by raising camels in terms of draft, milk, meat, hides and wool from centuries. There are different breeds of dromedary camels distributed in various parts of Pakistan; however there have been scarcity of research work on camels in Pakistan. Identification of novel link between Camel breeders with fatal neurodegenerative disorders is presence or not can be detect by a Prion gene and it was not carried out in Pakistan soil to date. Prion diseases which are a group of fatal neurodegenerative disorders affect both animals and humans. It is believed that the prions are infectious agents responsible for transmissible spongiform encephalopathies. In this study we report the first study on Prion protein gene in dromedary camels of Pakistan.Material, Methods & Results: Genes are the blueprint of life and determine the functional aspects of cellular mechanisms. Genomic DNA of the enrolled blood samples was extracted using the Nucleospin® DNA extraction kit. Genomic DNA was run on Agarose gel electrophoresis, checked the Genomic DNA quality and amplified using prion region specific primer pair. Prion protein gene was amplified (770 bp) in 35 individuals of seven dromedary camel breeds from the province Balochistan and Punjab of Pakistan. Samples having required fragment size were selected and sent for sequencing through Sanger Sequencing. All the sequences were aligned through multiple sequence alignment and edited using Codon Code aligner and explored for phylogenetic analysis. A portion of 667 bp was finally selected for phylogenetic analysis of dromedary camels from Pakistan with 61 different mammalian species (drawn from GenBank) that revealed five different clades. We found 99.9% nucleotide sequence similarities among Dromedary camels (Germany), Dromedary camels (Iran), and Dromedary camels (Pakistan). We observed deletion in dromedary camels in codon region 66-69 except wild Bactrian camels that might be the causative factor for Prion protein gene resistance in camels. The Neighbor-Joining method with bootstrap analysis of 1000 replicates was used to draw phylogenetic tree.Discussion: This study documents the presence of 14 PrP polymorphisms and shows the relationship between different camel breeds. The deletion had not previously been examined PrP allelic variation, and was found to segregate in these breeds. However, additional data are necessary to demonstrate PrP and genetic approach will be ideal for the future studies and, more investigations are necessary to demonstrate PrP genetic resistance in camels. The utility of these techniques in identifying Prion genes and selecting superior animals and culling the weak animals and making them parents of the next generation that will lead to producing more meat and milk with fewer animals are well discussed and by slaughtering of high Prion incidence will eliminate human animal fatal neurodegenerative disorders.

scholarly journals Complete Genome Sequence of a Genotype XVII Newcastle Disease Virus, Isolated from an Apparently Healthy Domestic Duck in Nigeria

2016 ◽  
Vol 4 (1) ◽  
Author(s):  
Ismaila Shittu ◽  
Poonam Sharma ◽  
Tony M. Joannis ◽  
Jeremy D. Volkening ◽  
Georgina N. Odaibo ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Genome Sequence ◽  
Newcastle Disease ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Protein Gene ◽  
Domestic Duck ◽  
Apparently Healthy

The first complete genome sequence of a strain of Newcastle disease virus (NDV) of genotype XVII is described here. A velogenic strain (duck/Nigeria/903/KUDU-113/1992) was isolated from an apparently healthy free-roaming domestic duck sampled in Kuru, Nigeria, in 1992. Phylogenetic analysis of the fusion protein gene and complete genome classified the isolate as a member of NDV class II, genotype XVII.

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