scholarly journals Fluorescence polarization in studies of bacterial cytoplasmic membrane fluidity under environmental stress

2007 ◽  
Vol 95 (1-3) ◽  
pp. 60-82 ◽  
Author(s):  
N.C.S. Mykytczuk ◽  
J.T. Trevors ◽  
L.G. Leduc ◽  
G.D. Ferroni
Keyword(s):  
Environmental Stress ◽  
Membrane Fluidity ◽  
Fluorescence Polarization ◽  
Cytoplasmic Membrane

scholarly journals pH Stress Alters Cytoplasmic Membrane Fluidity and atpB Gene Expression in Streptococcus mutans

2017 ◽  
Vol 27 (1) ◽  
pp. 15-22 ◽  
Author(s):  
Chul Min Cho ◽  
Seung Il Jung ◽  
Myung Sup Kim ◽  
Sae A Lee ◽  
Jung Sook Kang
Keyword(s):  
Gene Expression ◽  
Membrane Fluidity ◽  
Streptococcus Mutans ◽  
Cytoplasmic Membrane ◽  
Ph Stress

scholarly journals Membrane Fluidity Adjustments in Ethanol-Stressed Oenococcus oeni Cells

2003 ◽  
Vol 69 (10) ◽  
pp. 5826-5832 ◽  
Author(s):  
M. Graça Da Silveira ◽  
Elena A. Golovina ◽  
Folkert A. Hoekstra ◽  
Frank M. Rombouts ◽  
Tjakko Abee
Keyword(s):  
Membrane Fluidity ◽  
Cytoplasmic Membrane ◽  
De Novo ◽  
Oenococcus Oeni ◽  
Resonance Spectroscopy ◽  
Water Interface ◽  
Membrane Composition ◽  
Intact Cells ◽  
Short Term Adaptation ◽  
Tolerance To Ethanol

ABSTRACT The effect of ethanol on the cytoplasmic membrane of Oenococcus oeni cells and the role of membrane changes in the acquired tolerance to ethanol were investigated. Membrane tolerance to ethanol was defined as the resistance to ethanol-induced leakage of preloaded carboxyfluorescein (cF) from cells. To probe the fluidity of the cytoplasmic membrane, intact cells were labeled with doxyl-stearic acids and analyzed by electron spin resonance spectroscopy. Although the effect of ethanol was noticeable across the width of the membrane, we focused on fluidity changes at the lipid-water interface. Fluidity increased with increasing concentrations of ethanol. Cells responded to growth in the presence of 8% (vol/vol) ethanol by decreasing fluidity. Upon exposure to a range of ethanol concentrations, these adapted cells had reduced fluidity and cF leakage compared with cells grown in the absence of ethanol. Analysis of the membrane composition revealed an increase in the degree of fatty acid unsaturation and a decrease in the total amount of lipids in the cells grown in the presence of 8% (vol/vol) ethanol. Preexposure for 2 h to 12% (vol/vol) ethanol also reduced membrane fluidity and cF leakage. This short-term adaptation was not prevented in the presence of chloramphenicol, suggesting that de novo protein synthesis was not involved. We found a strong correlation between fluidity and cF leakage for all treatments and alcohol concentrations tested. We propose that the protective effect of growth in the presence of ethanol is, to a large extent, based on modification of the physicochemical state of the membrane, i.e., cells adjust their membrane permeability by decreasing fluidity at the lipid-water interface.

scholarly journals Identification of Plasmalogens in the Cytoplasmic Membrane of Bifidobacterium animalis subsp. lactis

2011 ◽  
Vol 78 (3) ◽  
pp. 880-884 ◽  
Author(s):  
Taylor S. Oberg ◽  
Robert E. Ward ◽  
James L. Steele ◽  
Jeff R. Broadbent
Keyword(s):  
Oxidative Stress ◽  
Environmental Stress ◽  
Stress Resistance ◽  
Cell Membranes ◽  
Cytoplasmic Membrane ◽  
Eukaryotic Cell ◽  
Bacterial Membrane ◽  
Membrane Composition ◽  
Content Type ◽  
Bifidobacterium Animalis

ABSTRACTPlasmalogens are ether-linked lipids that may influence oxidative stress resistance of eukaryotic cell membranes. Since bacterial membrane composition can influence environmental stress resistance, we explored the prevalence of plasmalogens in the cytoplasmic membrane ofBifidobacterium animalissubsp.lactis. Results showed plasmalogens are a major component of theB. animalissubsp.lactismembrane.

Membrane fluidity of Toxoplasma gondii: a fluorescence polarization study

1988 ◽  
Vol 62 (1) ◽  
pp. 11-15 ◽  
Author(s):  
Yves Gallois ◽  
Françoise Foussard ◽  
Andrée Girault ◽  
Jocelyne Hodbert ◽  
Anne Tricaud ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Membrane Fluidity ◽  
Fluorescence Polarization ◽  
Polarization Study

Cellular membrane fluidity measurement by fluorescence polarization in indomethacin-induced gastric cellular injury in vitro

2007 ◽  
Vol 42 (12) ◽  
pp. 939-946 ◽  
Author(s):  
Tsuyoshi Kaneko ◽  
Hirofumi Matsui ◽  
Osamu Shimokawa ◽  
Akira Nakahara ◽  
Ichinosuke Hyodo
Keyword(s):  
Membrane Fluidity ◽  
Fluorescence Polarization ◽  
Cellular Membrane ◽  
Cellular Injury

Factors altering the membrane fluidity of spinach thylakoid as determined by fluorescence polarization

2011 ◽  
Vol 33 (3) ◽  
pp. 1019-1024 ◽  
Author(s):  
Zhi Fang Lin ◽  
Nan Liu ◽  
Gui Zhu Lin ◽  
Chang Lian Peng
Keyword(s):  
Membrane Fluidity ◽  
Fluorescence Polarization ◽  
Spinach Thylakoid

scholarly journals The effect of a dietary supplement ofn-3 polyunsaturated fat on platelet lipid composition, platelet function and platelet plasma membrane fluidity in healthy volunteers

1988 ◽  
Vol 60 (1) ◽  
pp. 5-12 ◽  
Author(s):  
M. J. Gibney ◽  
Caroline Bolton-Smith
Keyword(s):  
Fatty Acids ◽  
Plasma Membrane ◽  
Fish Oil ◽  
Membrane Fluidity ◽  
Fluorescence Polarization ◽  
Total Phospholipid ◽  
Phosphatidyl Inositol ◽  
Plasma Membrane Fluidity ◽  
Fish Oil Supplementation ◽  
Platelet Thromboxane

1. Eight healthy male volunteers (aged 22–39 years) supplemented their normal daily diet with 15 g encapsulated fish oil (MaxEPA) for a 6 week period. Fasting blood samples were taken before, at the completion of and 3 months after the period of supplementation.2. Evaluation of nutrient intakes showed that the intake ofn-3 polyunsaturated fatty acids rose significantly (P< 0·01) during supplementation. This was reflected in changes in the fatty acid composition of platelet phosphatidyl choline (PC) and phosphatidyl ethanolamine (PE) without any changes in phosphatidyl serine, phosphatidyl inositol or sphingomyelin.3. In both PC and PE there were significant (P< 0·05) increases in the levels of 18:1n-9 and 20:5n-3 fatty acids and a significant (P< 0·05) decrease in 20:4n-6 during supplementation. 16:0 rose significantly in PC (P< 0·05) while in PE, 18:0 fell and both 22:5n-3 and 22:6n-3 rose significantly (P< 0·05).4. There were no significant effects of fish-oil supplementation on serum lipids, platelet cholesterol: phospholipid, collagen-induced platelet aggregation or collagen-induced platelet thromboxane B2production. However, there was a significant correlation (P< 0·001; r+0·63) between total phospholipid arachidonic acid and platelet thromboxane production.5. The fluorescent probe 1,6-diphenyl-1,3,5-hexatriene was used to determine whether fish-oil supplementation altered fluorescence polarization of isolated platelet plasma membrane and, by inference, platelet plasma membrane fluidity. No significant effect of fish-oil supplementation on fluorescence polarization was seen.

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