Fluorescence analysis of a tumor model in the chorioallantoic membrane used for the evaluation of different photosensitizers for photodynamic therapy

Vitexicarpin (VIT) isolated from the fruits ofVitex rotundifoliahas shown antitumor, anti-inflammatory, and immunoregulatory properties. This work is designed to evaluate the antiangiogenic effects of VIT and address the underlying action mechanism of VIT by a network pharmacology approach. The results validated that VIT can act as a novel angiogenesis inhibitor. Firstly, VIT can exert good antiangiogenic effects by inhibiting vascular-endothelial-growth-factor- (VEGF-) induced endothelial cell proliferation, migration, and capillary-like tube formation on matrigel in a dose-dependent manner. Secondly, VIT was also shown to have an antiangiogenic mechanism through inhibition of cell cycle progression and induction of apoptosis. Thirdly, VIT inhibited chorioallantoic membrane angiogenesis as well as tumor angiogenesis in an allograft mouse tumor model. We further addressed VIT’s molecular mechanism of antiangiogenic actions using one of our network pharmacology methods named drugCIPHER. Then, we tested some key molecules in the VEGF pathway targeted by VIT and verified the inhibition effects of VIT on AKT and SRC phosphorylation. Taken together, this work not only identifies VIT as a novel potent angiogenesis inhibitor, but also demonstrates that network pharmacology methods can be an effective and promising approach to make discovery and understand the action mechanism of herbal ingredients.

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Vascular Effects of Photodynamic Therapy with Curcumin in a Chorioallantoic Membrane Model

International Journal of Molecular Sciences ◽

10.3390/ijms20051084 ◽

2019 ◽

Vol 20 (5) ◽

pp. 1084 ◽

Cited By ~ 9

Author(s):

Hilde Buzzá ◽

Lucas Fialho de Freitas ◽

Lilian Moriyama ◽

Ramon Teixeira Rosa ◽

Vanderlei Bagnato ◽

...

Keyword(s):

Photodynamic Therapy ◽

Molecular Oxygen ◽

Chorioallantoic Membrane ◽

Topical Administration ◽

Inhibitory Effect ◽

Vascular Effects ◽

Vascular Effect ◽

Chick Chorioallantoic Membrane ◽

Anti Oxidant ◽

Total Fluence

Photodynamic Therapy (PDT) is a treatment that requires light, a photosensitizing agent, and molecular oxygen. The photosensitizer is activated by light and it interacts with the oxygen that is present in the cellular microenvironment. The molecular oxygen is transformed into singlet oxygen, which is highly reactive and responsible for the cell death. Therefore, PS is an important element for the therapy happens, including its concentration. Curcumin is a natural photosensitizer and it has demonstrated its anti-inflammatory and anti-oxidant effects that inhibit several signal transduction pathways. PDT vascular effects of curcumin at concentrations varying from 0.1 to 10 mM/cm2 and topical administration were investigated in a chick Chorioallantoic Membrane (CAM) model. The irradiation was performed at 450 nm, irradiance of 50 mW/cm2 during 10 min, delivering a total fluence of 30 J/cm2. The vascular effect was followed after the application of curcumin, with images being obtained each 30 min in the first 3 h, 12 h, and 24 h. Those images were qualitatively and quantitatively analyzed with a MatLAB®. Curcumin was expected to exhibit a vascular effect due to its angio-inhibitory effect. Using curcumin as photosensitizer, PDT induced a higher and faster vascular effect when compared to the use of this compound alone.

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Patient Derived Chicken Egg Tumor Model (PDcE Model): Current Status and Critical Issues

Cells ◽

10.3390/cells8050440 ◽

2019 ◽

Vol 8 (5) ◽

pp. 440 ◽

Cited By ~ 10

Author(s):

Aoi Komatsu ◽

Kotaro Matsumoto ◽

Tomoki Saito ◽

Manabu Muto ◽

Fuyuhiko Tamanoi

Keyword(s):

Cancer Patients ◽

Chorioallantoic Membrane ◽

Tumor Model ◽

Tumor Formation ◽

Current Status ◽

Cam Assay ◽

Chicken Egg ◽

Critical Issues ◽

Chicken Eggs ◽

Chorioallantoic Membrane Assay

Chorioallantoic membrane assay (CAM assay) using fertilized chicken eggs has been used for the study of tumor formation, angiogenesis and metastasis. Recently, there is growing realization that this system provides a valuable assay for a patient-derived tumor model. Several reports establish that tumor samples from cancer patients can be used to reproduce tumor in the chicken egg. High transplantation efficiency has been achieved. In this review, we discuss examples of transplanting patient tumors. We then discuss critical issues that need to be addressed to pursue this line of experiments. The patient-derived chicken egg model (PDcE model) has an advantage over other models in its rapid tumor formation. This raises the possibility that the PDcE model is valuable for identifying optimum drug for each individual patient.

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