scholarly journals Vascular Effects of Photodynamic Therapy with Curcumin in a Chorioallantoic Membrane Model

2019 ◽  
Vol 20 (5) ◽  
pp. 1084 ◽  
Author(s):  
Hilde Buzzá ◽  
Lucas Fialho de Freitas ◽  
Lilian Moriyama ◽  
Ramon Teixeira Rosa ◽  
Vanderlei Bagnato ◽  
...  

Photodynamic Therapy (PDT) is a treatment that requires light, a photosensitizing agent, and molecular oxygen. The photosensitizer is activated by light and it interacts with the oxygen that is present in the cellular microenvironment. The molecular oxygen is transformed into singlet oxygen, which is highly reactive and responsible for the cell death. Therefore, PS is an important element for the therapy happens, including its concentration. Curcumin is a natural photosensitizer and it has demonstrated its anti-inflammatory and anti-oxidant effects that inhibit several signal transduction pathways. PDT vascular effects of curcumin at concentrations varying from 0.1 to 10 mM/cm2 and topical administration were investigated in a chick Chorioallantoic Membrane (CAM) model. The irradiation was performed at 450 nm, irradiance of 50 mW/cm2 during 10 min, delivering a total fluence of 30 J/cm2. The vascular effect was followed after the application of curcumin, with images being obtained each 30 min in the first 3 h, 12 h, and 24 h. Those images were qualitatively and quantitatively analyzed with a MatLAB®. Curcumin was expected to exhibit a vascular effect due to its angio-inhibitory effect. Using curcumin as photosensitizer, PDT induced a higher and faster vascular effect when compared to the use of this compound alone.

2005 ◽  
Vol 28 (6) ◽  
pp. 1054-1060 ◽  
Author(s):  
Constance Lay Lay Saw ◽  
Malini Olivo ◽  
William Wei Lim Chin ◽  
Khee Chee Soo ◽  
Paul Wan Sia Heng

1975 ◽  
Vol 141 (2) ◽  
pp. 427-439 ◽  
Author(s):  
H Brem ◽  
J Folkman

Capillary proliferation induced by tumor is shown to be inhibited by neonatal scapular cartilage. Using the rabbit cornea as an assay, the cartilage implant decreased the rate of capillary growth, induced by tumor, by an average of 75%. Vascularization was prevented completely in 28% of tumors. The inhibitory effect of small cartilage implants operates over distances of up to 2.0 mm and displays a gradient from the cartilage source. The experiments suggest that the cartilage inhibitor does not antagonize tumor angiogenesis factor, but appears to inhibit capillary proliferation directly. The inhibitory material does not elicit an inflammatory response in either the rabbit cornea or in the chick chorioallantoic membrane. Thus with further purification, it may prove useful as a means of maintining tumor dormancy by "antiangiogenesis."


2020 ◽  
Vol 394 (1) ◽  
pp. 2000046
Author(s):  
Lays Fernanda Nunes Dourado ◽  
Carolina Nunes da Silva ◽  
Raquel Gregorio Arribada ◽  
Armando da Silva Cunha Júnior

1992 ◽  
Vol 12 (4) ◽  
pp. 432-440 ◽  
Author(s):  
S. Kimel ◽  
L. O. Svaasand ◽  
M. Hammer-Wilson ◽  
V. Gottfried ◽  
S. Cheng ◽  
...  

1991 ◽  
Author(s):  
Sol Kimel ◽  
Lars O. Svaasand ◽  
Marie J. Hammer-Wilson ◽  
Varda Gottfried ◽  
Michael W. Berns

2020 ◽  
Author(s):  
busenur Aslanoglu ◽  
Ilya Yakavets ◽  
Vladimir Zorin ◽  
Henri-Pierre Lassalle ◽  
Francesca Ingrosso ◽  
...  

Computational tools have been used to study the photophysical and photochemical features of photosensitizers in photodynamic therapy (PDT) –a minimally invasive, less aggressive alternative for cancer treatment. PDT is mainly based by the activation of molecular oxygen through the action of a photoexcited sensitizer (photosensitizer). Temoporfin, widely known as mTHPC, is a second-generation photosensitizer, which produces the cytotoxic singlet oxygen when irradiated with visible light and hence destroys tumor cells. However, the bioavailability of the mostly hydrophobic photosensitizer, and hence its incorporation into the cells, is fundamental to achieve the desired effect on malignant tissues by PDT. In this study, we focus on the optical properties of the temoporfin chromophore in different environments –in <i>vacuo</i>, in solution, encapsulated in drug delivery agents, namely cyclodextrin, and interacting with a lipid bilayer.


Sign in / Sign up

Export Citation Format

Share Document