scholarly journals Purification and characterization of a collagenase from Penicillium sp. UCP 1286 by polyethylene glycol-phosphate aqueous two-phase system

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An aqueous two-phase system of polyethylene glycol (PEG)/(NH4)2SO4 with the behavior of forming aqueous two-phase and the distribution rules of potassium glycyrrhizinate in the aqueous two-phase system were studied. Effects of the molecular weight of PEG, the mass fraction of PEG and (NH4)2SO4, and pH value were investigated. The result show that when the mass fraction of PEG1000 was 50%, the mass fraction of (NH4)2SO4 was 5.7%, and pH value was 6.5, the highest extraction recovery of potassium glycyrrhizinate could reach 99.7%. This aqueous two-phase extraction may provide a new technique for separation of potassium glycyrrhizinate.


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