scholarly journals A new vector coupling ligation-independent cloning with sortase a fusion for efficient cloning and one-step purification of tag-free recombinant proteins

2019 ◽  
Vol 161 ◽  
pp. 1-7 ◽  
Author(s):  
Xinying Jia ◽  
Theo Crawford ◽  
Alan H. Zhang ◽  
Mehdi Mobli
Keyword(s):  
Recombinant Proteins ◽  
Sortase A ◽  
Vector Coupling ◽  
Ligation Independent Cloning ◽  
One Step

Stable one-step technetium-99m labeling of His-tagged recombinant proteins with a novel Tc(I)–carbonyl complex

10.1038/12890 ◽  
1999 ◽  
Vol 17 (9) ◽  
pp. 897-901 ◽  
Author(s):  
Robert Waibel ◽  
Roger Alberto ◽  
Jörg Willuda ◽  
Ricarda Finnern ◽  
Roger Schibli ◽  
...  
Keyword(s):  
Recombinant Proteins ◽  
Carbonyl Complex ◽  
Technetium 99M ◽  
One Step

One-Step Purification of Recombinant Proteins Using a Nanomolar-Affinity Streptavidin-Binding Peptide, the SBP-Tag

2001 ◽  
Vol 23 (3) ◽  
pp. 440-446 ◽  
Author(s):  
Anthony D. Keefe ◽  
David S. Wilson ◽  
Burckhard Seelig ◽  
Jack W. Szostak
Keyword(s):  
Recombinant Proteins ◽  
Binding Peptide ◽  
One Step ◽  
Streptavidin Binding

One-Step Purification of Recombinant Proteins with the 6xHis Tag and Ni-NTA Resin

2003 ◽  
pp. 491-510 ◽  
Author(s):  
Joanne Crowe ◽  
Brigitte Steude Masone ◽  
Joachim Ribbe
Keyword(s):  
Recombinant Proteins ◽  
One Step

scholarly journals One-Step Sequence- and Ligation-Independent Cloning as a Rapid and Versatile Cloning Method for Functional Genomics Studies

2012 ◽  
Vol 78 (15) ◽  
pp. 5440-5443 ◽  
Author(s):  
Jae-Yeon Jeong ◽  
Hyung-Soon Yim ◽  
Ji-Young Ryu ◽  
Hyun Sook Lee ◽  
Jung-Hyun Lee ◽  
...  
Keyword(s):  
Functional Genomics ◽  
Dna Polymerase ◽  
Room Temperature ◽  
Cost Effective ◽  
Time Saving ◽  
Ligation Independent Cloning ◽  
Effective Time ◽  
Cloning Method ◽  
Directional Cloning ◽  
One Step

ABSTRACTWe developed one-step sequence- and ligation-independent cloning (SLIC) as a simple, cost-effective, time-saving, and versatile cloning method. Highly efficient and directional cloning can be achieved by direct bacterial transformation 2.5 min after mixing any linearized vector, an insert(s) prepared by PCR, and T4 DNA polymerase in a tube at room temperature.

scholarly journals Simplifying plant gene silencing and genome editing logistics by a one-Agrobacterium system for simultaneous delivery of multipartite virus vectors

2021 ◽  
Author(s):  
Verónica Aragonés ◽  
Flavio Aliaga ◽  
Fabio Pasin ◽  
José-Antonio Daròs
Keyword(s):  
Gene Silencing ◽  
Genome Editing ◽  
Recombinant Proteins ◽  
Viral Vectors ◽  
Tobacco Rattle Virus ◽  
Vector System ◽  
Efficient Production ◽  
Virus Induced Gene Silencing ◽  
Guide Rnas ◽  
One Step

Genome editing and gene expression engineering using CRISPR-Cas systems in plants usually rely on labor-intensive tissue culture approaches to generate stably transformed plants that express the components of the reaction. Viral vectors have demonstrated to be a quick and effective alternative to express multiple guide RNAs, DNA templates for homologous recombination, and even Cas nucleases. Here we have developed an improved vector system based on tobacco rattle virus (TRV) to simplify logistics in genome editing and gene silencing approaches. The new system consists in a single Agrobacterium tumefaciens clone co-transformed with two compatible mini binary vectors from which TRV RNA1 and an engineered version of TRV RNA2 are expressed. Sequences of recombinant proteins, gene fragments for virus-induced gene silencing (VIGS) or guide RNAs can be easily inserted by one-step digestion-ligation and homology-based cloning methods in the RNA2 plasmid to produce vectors with a size substantially smaller than usual. Using this new one-Agrobacterium TRV mini vector system, we show robust VIGS of an endogenous host gene after infiltration of bacterial suspensions at low optical densities, and efficient production of recombinant proteins in Nicotiana benthamiana. Most importantly, we also show highly efficient heritable genome editing in more than half of the seedling originating from inoculated N. benthamiana plants that express Cas9.

scholarly journals New ligation independent cloning vectors for expression of recombinant proteins with a self-cleaving CPD/6xHis-tag

2017 ◽  
Vol 17 (1) ◽  
Author(s):  
Marco Biancucci ◽  
Jazel S. Dolores ◽  
Jennifer Wong ◽  
Sarah Grimshaw ◽  
Wayne F. Anderson ◽  
...  
Keyword(s):  
Recombinant Proteins ◽  
Cloning Vectors ◽  
Ligation Independent Cloning

Rapid Production of Functionalized Recombinant Proteins:  Marrying Ligation Independent Cloning and in Vitro Protein Ligation

2006 ◽  
Vol 17 (3) ◽  
pp. 610-617 ◽  
Author(s):  
Susanna Kushnir ◽  
Yoann Marsac ◽  
Reinhard Breitling ◽  
Igor Granovsky ◽  
Vera Brok-Volchanskaya ◽  
...  
Keyword(s):  
Recombinant Proteins ◽  
Protein Ligation ◽  
Ligation Independent Cloning ◽  
Rapid Production ◽  
Vitro Protein

Preparation of affinity membranes using thermally induced phase separation for one-step purification of recombinant proteins

2013 ◽  
Vol 434 (2) ◽  
pp. 269-274 ◽  
Author(s):  
Takafumi Honjo ◽  
Kazuki Hoe ◽  
Shunsuke Tabayashi ◽  
Tsutomu Tanaka ◽  
Josui Shimada ◽  
...  
Keyword(s):  
Phase Separation ◽  
Recombinant Proteins ◽  
Thermally Induced Phase Separation ◽  
Thermally Induced ◽  
Affinity Membranes ◽  
One Step

Selenolthiol and Dithiol C-Terminal Tetrapeptide Motifs for One-Step Purification and Labeling of Recombinant Proteins Produced in E. coli

ChemBioChem ◽  
2006 ◽  
Vol 7 (12) ◽  
pp. 1976-1981 ◽  
Author(s):  
Qing Cheng ◽  
Linda Johansson ◽  
Jan-Olov Thorell ◽  
Anna Fredriksson ◽  
Erik Samén ◽  
...  
Keyword(s):  
Recombinant Proteins ◽  
E Coli ◽  
One Step
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