Impaired fetal organ development is linked to altered placental morphology and function in a systemic hsFLT1-transgenic preeclampsia/fetal growth restriction mouse model: the placenta as a modulator of offspring health?

Placenta ◽  
2021 ◽  
Vol 112 ◽  
pp. e26
Author(s):  
Rebekka Vogtmann ◽  
Meray Serdar ◽  
Monia Dewan ◽  
Gemma Comas-Armangué ◽  
Mian Bao ◽  
...  
Keyword(s):  
Mouse Model ◽  
Fetal Growth ◽  
Fetal Growth Restriction ◽  
Growth Restriction ◽  
Organ Development ◽  
Fetal Organ ◽  
And Function ◽  
Offspring Health

scholarly journals Cigarette exposure induces changes in maternal vascular function in a pregnant mouse model

2010 ◽  
Vol 298 (5) ◽  
pp. R1249-R1256 ◽  
Author(s):  
Robin E. Gandley ◽  
Arun Jeyabalan ◽  
Ketaki Desai ◽  
Stacy McGonigal ◽  
Jennifer Rohland ◽  
...  
Keyword(s):  
Mouse Model ◽  
Cigarette Smoke ◽  
Fetal Growth ◽  
Fetal Growth Restriction ◽  
Smoke Exposure ◽  
Renal Arteries ◽  
Growth Restriction ◽  
Whole Body ◽  
Cigarette Smoke Exposure ◽  
Pregnant Mice

Smoking is associated with multiple adverse pregnancy outcomes, including fetal growth restriction. The objective of this study was to determine whether cigarette smoke exposure during pregnancy in a mouse model affects the functional properties of maternal uterine, mesenteric, and renal arteries as a possible mechanism for growth restriction. C57Bl/CJ mice were exposed to whole body sidestream smoke for 4 h/day. Smoke particle exposure was increased from day 4 of gestation until late pregnancy ( day 16–19), with mean total suspended particle levels of 63 mg/m3, representative of moderate-to-heavy smoking in humans. Uterine, mesenteric, and renal arteries from late-pregnant and virgin mice were isolated and studied in a pressure-arteriograph system ( n = 23). Plasma cotinine was measured by ELISA. Fetal weights were significantly reduced in smoke-exposed compared with control fetuses (0.88 ± 0.1 vs. 1.0 ± 0.08 g, P < 0.02), while litter sizes were not different. Endothelium-mediated relaxation responses to methacholine were significantly impaired in both the uterine and mesenteric vasculature of pregnant mice exposed to cigarette smoke during gestation. This difference was not apparent in isolated renal arteries from pregnant mice exposed to cigarette smoke; however, relaxation was significantly reduced in renal arteries from smoke-exposed virgin mice. In conclusion, we found that passive cigarette smoke exposure is associated with impaired vascular relaxation of uterine and mesenteric arteries in pregnant mice. Functional maternal vascular perturbations during pregnancy, specifically impaired peripheral and uterine vasodilation, may contribute to a mechanism by which smoking results in fetal growth restriction.

scholarly journals Quantification of fetal organ sparing in maternal low-protein dietary models

2021 ◽  
Vol 6 ◽  
pp. 218
Author(s):  
Patricia Serpente ◽  
Ying Zhang ◽  
Eva Islimye ◽  
Sarah Hart-Johnson ◽  
Alex P. Gould
Keyword(s):  
Protein Content ◽  
Fetal Growth ◽  
Dietary Protein ◽  
Fetal Growth Restriction ◽  
Fetal Brain ◽  
Growth Restriction ◽  
Maternal Malnutrition ◽  
Fetal Organ ◽  
Low Protein ◽  
Organ Sparing

Background: Maternal malnutrition can lead to fetal growth restriction. This is often associated with organ sparing and long-lasting physiological dysfunctions during adulthood, although the underlying mechanisms are not yet well understood. Methods: Low protein (LP) dietary models in C57BL/6J mice were used to investigate the proximal effects of maternal malnutrition on fetal organ weights and organ sparing at embryonic day 18.5 (E18.5). Results:  Maternal 8% LP diet induced strikingly different degrees of fetal growth restriction in different animal facilities, but adjustment of dietary protein content allowed similar fetal body masses to be obtained. A maternal LP diet that restricted fetal body mass by 40% did not decrease fetal brain mass to the same extent, reflecting positive growth sparing of this organ. Under these conditions, fetal pancreas and liver mass decreased by 60-70%, indicative of negative organ sparing. A series of dietary swaps between LP and standard diets showed that the liver is capable of efficient catch-up growth from as late as E14.5 whereas, after E10.5, the pancreas is not. Conclusions: This study highlights that the reproducibility of LP fetal growth restriction studies between laboratories can be improved by careful calibration of maternal dietary protein content. LP diets that induce 30-40% restriction of prenatal growth provide a good model for fetal organ sparing. For the liver, recovery of growth following protein restriction is efficient throughout fetal development but, for the pancreas, transient LP exposures spanning the progenitor expansion phase lead to an irreversible fetal growth deficit.

USP22 regulates the formation and function of placental vasculature during the development of fetal growth restriction

Placenta ◽  
2021 ◽  
Author(s):  
Zhen Liu ◽  
Jingxue Wang ◽  
Yan Gao ◽  
Yongbing Guo ◽  
Yuchun Zhu ◽  
...  
Keyword(s):  
Fetal Growth ◽  
Fetal Growth Restriction ◽  
Growth Restriction ◽  
And Function

Characterization of fetal monocytes in preeclampsia and fetal growth restriction

2019 ◽  
Vol 47 (4) ◽  
pp. 434-438 ◽  
Author(s):  
Thushari I. Alahakoon ◽  
Heather Medbury ◽  
Helen Williams ◽  
Nicole Fewings ◽  
Xin M. Wang ◽  
...  
Keyword(s):  
Cord Blood ◽  
Fetal Growth ◽  
Fetal Growth Restriction ◽  
Normal Pregnancy ◽  
Growth Restriction ◽  
Clinical Group ◽  
Monocyte Subset ◽  
Maternal Circulation ◽  
Cross Sectional ◽  
And Function

AbstractBackgroundThere is little available data on fetal monocyte phenotype and function. A prospective cross-sectional pilot study was conducted to describe the cord blood monocyte subset phenotype in preeclampsia (PE) and fetal growth restriction (FGR) as compared to normal pregnancy and maternal circulation.MethodsMaternal and cord blood samples from 27 pregnancies were collected at delivery from normal pregnancy, PE, FGR and PE+FGR. The distribution of fetal monocyte subtypes was characterized by CD14 and CD16 expression using flow cytometry and compared for each clinical group using a classification of classical, intermediate and non-classical subsets.ResultsThe intermediate monocytes were the dominant monocyte subset in the cord blood of PE and PE+FGR with an increase in the combined inflammatory monocyte subsets intermediate and non-classical in PE compared to normal pregnancy. The non-classical monocyte subset proportion was elevated in all pathological groups PE, FGR and PE+FGR. A significant reduction in the non-classical monocyte subset was observed in the cord blood of the normal pregnancy group as compared to the maternal circulation.ConclusionThis study describes for the first time in the fetal circulation, dominant monocyte intermediate subsets and increased inflammatory subsets in PE as well as increased non-classical subsets in PE and FGR compared to normal pregnancy.

Maternal administration of Sildenafil Citrate alters fetal vascular function in a mouse model of fetal growth restriction

Placenta ◽  
2013 ◽  
Vol 34 (9) ◽  
pp. A22
Author(s):  
Lewis Renshall ◽  
Elizabeth Cowley ◽  
Susan Greenwood ◽  
Mark Dilworth ◽  
Mark Wareing
Keyword(s):  
Mouse Model ◽  
Fetal Growth ◽  
Fetal Growth Restriction ◽  
Vascular Function ◽  
Growth Restriction ◽  
Sildenafil Citrate ◽  
Maternal Administration

scholarly journals Sildenafil Therapy Normalizes the Aberrant Metabolomic Profile in the Comt−/− Mouse Model of Preeclampsia/Fetal Growth Restriction

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Joanna L. Stanley ◽  
Karolina Sulek ◽  
Irene J. Andersson ◽  
Sandra T. Davidge ◽  
Louise C. Kenny ◽  
...  
Keyword(s):  
Mouse Model ◽  
Fetal Growth ◽  
Fetal Growth Restriction ◽  
Growth Restriction ◽  
Metabolomic Profile

scholarly journals Sildenafil Citrate Increases Fetal Weight in a Mouse Model of Fetal Growth Restriction with a Normal Vascular Phenotype

PLoS ONE ◽  
2013 ◽  
Vol 8 (10) ◽  
pp. e77748 ◽  
Author(s):  
Mark Robert Dilworth ◽  
Irene Andersson ◽  
Lewis James Renshall ◽  
Elizabeth Cowley ◽  
Philip Baker ◽  
...  
Keyword(s):  
Mouse Model ◽  
Fetal Growth ◽  
Fetal Growth Restriction ◽  
Growth Restriction ◽  
Fetal Weight ◽  
Sildenafil Citrate ◽  
Vascular Phenotype
Sign in / Sign up

Export Citation Format

Share Document