The role of the bifunctional enzyme, fructose-6-phosphate-2-kinase/fructose-2,6-bisphosphatase, in carbon partitioning during salt stress and salt tolerance in Rice (Oryza sativa L.)

Salt-tolerant mutants are valuable resources for basic and applied research on plant salt tolerance. Here, we report the isolation and characterisation of a salt-tolerant rice (Oryza sativa L.) mutant. This mutant was identified from an ethyl methanesulfonate-induced Nipponbare mutant library, designated as rice salt tolerant 1 (rst1). The rst1 mutant was tolerant to salt stress and showed significantly higher shoot biomass and chlorophyll content, but lower lipid peroxidation and electrolyte leakage under NaCl stress. The improved salt tolerance of this mutant may be due mainly to its enhanced ability to restrict Na+ accumulation in shoots under salt stress conditions. Genetic analysis indicated that the salt tolerance of the rst1 mutant was controlled by a single recessive gene. Quantitative trait locus (QTL) mapping for salt tolerance was performed using an F2 population of rst1 × Peiai 64. Two QTLs were detected, in which the locus on chromosome 6 was determined to be the candidate locus of the rst1 gene. The rst1 locus was subsequently shown to reside within a 270.4-kb region defined by the markers IM29432 and IM29702. This result will be useful for map-based cloning of the rst1 gene and for marker-assisted breeding for salt tolerance in rice.

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The role of root apoplastic transport barriers in salt tolerance of rice (Oryza sativa L.)

Planta ◽

10.1007/s00425-009-0930-6 ◽

2009 ◽

Vol 230 (1) ◽

pp. 119-134 ◽

Cited By ~ 116

Author(s):

Pannaga Krishnamurthy ◽

Kosala Ranathunge ◽

Rochus Franke ◽

H. S. Prakash ◽

Lukas Schreiber ◽

...

Keyword(s):

Oryza Sativa ◽

Salt Tolerance ◽

Oryza Sativa L ◽

Transport Barriers

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Photosynthetic Performance in Improved ‘KDML105’ Rice (Oryza sativa L.) Lines Containing Drought and Salt Tolerance Genes under Drought and Salt Stress

Pertanika Journal of Tropical Agricultural Science ◽

10.47836/pjtas.43.4.17 ◽

2020 ◽

Vol 43 (4) ◽

Author(s):

Dechudom Pamuta ◽

Meechai Siangliw ◽

Jirawat Sanitchon ◽

Jarunjit Pengrat ◽

Jonaliza L. Siangliw ◽

...

Keyword(s):

Oryza Sativa ◽

Salt Stress ◽

Drought Stress ◽

Salt Tolerance ◽

Quantum Yield ◽

Oryza Sativa L ◽

Photosynthetic Performance ◽

Psii Photochemistry ◽

Drought And Salt Stress ◽

Quantum Yield Of Psii

Rice (Oryza sativa L.) ‘KDML105’ is the most popular aromatic rice originating in Thailand. This cultivar is highly susceptible to abiotic stresses, especially drought and salt stress during the seedling stage. The objective of this study was to investigate the photosynthetic performance in response to drought and salt stress of four improved breeding lines, specifically CSSL94 and CSSL103 (containing drought-tolerant quantitative trait loci: DT-QTLs) and RGD1 and RGD4 (containing a salt-tolerance gene, SKC1), with ‘KDML105’ (susceptible) and DH103 (tolerant to drought and salt stress) as the controls. Rice seedlings were grown for 21 days in hydroponic solutions and then exposed to salt stress (150 mM NaCl) or drought stress (20% PEG6000) for 10 days. The results indicated that when subjected to drought and salt stress, all rice lines/cultivar exhibited significant reductions in net photosynthesis rate (PN), stomatal conductance (gs), transpiration rate (E), the maximal quantum yield of PSII photochemistry (Fv/Fm), the effective quantum yield of PSII photochemistry (Fv’/Fm’), photosynthetic pigments, and SPAD readings, whereas water use efficiency (WUE) and non-photochemical quenching (NPQ) increased. Compared with ‘KDML105’, CSSL94, and CSSL103 were more tolerant to both drought and salinity, showing less reduction in all photosynthetic parameters. For RGD1 and RGD4, it was confirmed that these lines had a higher level of salt tolerance than ‘KDML105’ based on better photosynthetic performance under salt stress, demonstrating that these lines were also more tolerant to drought stress.

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Salt Tolerance of Barak Cenana Rice (Oryza sativa L cv. Barak Cenana) EMS Derived M1 Putative Mutants

KnE Life Sciences ◽

10.18502/kls.v5i2.6441 ◽

2020 ◽

Author(s):

Ida Bagus Made Artadana ◽

Aurelia Nadine Handoyo ◽

Popy H. Hardjo ◽

Maria Goretti Purwanto

Keyword(s):

Oryza Sativa ◽

Salt Stress ◽

Salt Tolerance ◽

Oryza Sativa L ◽

Rice Cultivar ◽

Red Rice ◽

Random Mutation ◽

Tolerance Level ◽

Stable Mutant ◽

Mutant Lines

Barak Cenana (Oryza sativa L cv. Barak Cenana) is a native red rice cultivar of Tabanan regency, Bali. We have previously created first-generation putative mutants (M1) lines of Barak Cenana using EMS. In this study, we aim to evaluate M1 lines tolerance to salt stress. Three-leaf seedlings of WT Barak Cenana and M1 lines were cultured in hydroponic system containing Yoshida solution and 100 mM NaCl for 7 days. The salt tolerance level of each seedling was evaluated using SES standard and the survived seedlings were transferred into the pot-containing soil growth for maturation. All WT were died when treated with 100 mM NaCl for 7 days. In contrast, some M1 lines were survived where 0.5% were highly tolerance (II2A-4) and 4.06% (1F-4, 1F-3, II2A-8, IID-1, IID-4, IID-6 and 1B-6) were tolerance to salt stress. This variation of salt tolerance level among M1 lines is likely due to the random mutation caused by EMS. Furthermore, all survived mutants were fertile and able to produce mature seeds. As characters in M1 generation are not stable, future studies are required to establish stable mutant lines. Keywords: Rice, EMS, Mutants, Barak Cenana, salt tolerance

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