Cloning of FaPAL6 gene from strawberry fruit and characterization of its expression and enzymatic activity in two cultivars with different anthocyanin accumulation

Isolation and characterization of bacteria in food products are important to determine and distinguish the beneficial or harmful effects of microbiota in certain samples. Lactic acid bacteria in food products had long been associated to good factors as food preservatives and with added fermentation metabolites. This study isolated and characterized lactic acid bacteria from burong bangus. The culture and purification process of bacteria isolation resulted to 4 strains of lactic acid bacteria namely Enterococcus faecalis, Tetragenococcus muriaticus, Lactobacillus delbrueckii subp. delbrueckii and Carnobacterium divergens. High enzymatic activity were observed with E. faecalis particularly on lipase and protease assay. While C. divergens have no enzymatic activity against lipase, protease, amylase and cellulase. The antimicrobial property of L. delbrueckii is only susceptible to amoxicillin unlike the other three bacteria isolates. No antagonistic activity were observed with the four bacterial strains against Bacillus subtilis, Staphylococcus aureus and Escherichia coli. The result of this study showed promising benefits to the industry especially in developing countries like the Philippines because population are not yet so aware of this organisms and the benefits that can be derived through their consumption.

Download Full-text

Partial characterization of the enzymatic activity associated with the binary toxin (type C2) produced by Clostridium botulinum.

Infection and Immunity ◽

10.1128/iai.56.1.24-27.1988 ◽

1988 ◽

Vol 56 (1) ◽

pp. 24-27 ◽

Cited By ~ 4

Author(s):

L L Simpson ◽

H Zepeda ◽

I Ohishi

Keyword(s):

Enzymatic Activity ◽

Clostridium Botulinum ◽

Partial Characterization ◽

Binary Toxin

Download Full-text

Cloning and Characterization of cDNAs from Genes Differentially Expressed during the Strawberry Fruit Ripening Process by a MAST-PCR-SBDS Method

Analytical Biochemistry ◽

10.1006/abio.1997.2110 ◽

1997 ◽

Vol 248 (2) ◽

pp. 288-296 ◽

Cited By ~ 32

Author(s):

N. Medina-Escobar ◽

J. Cárdenas ◽

V. Valpuesta ◽

J. Muñoz-Blanco ◽

J.L. Caballero

Keyword(s):

Fruit Ripening ◽

Differentially Expressed ◽

Strawberry Fruit ◽

Ripening Process ◽

Strawberry Fruit Ripening

Download Full-text

Biochemical characterization of selected plant species from Brazilian Savannas

Brazilian Archives of Biology and Technology ◽

10.1590/s1516-89132004000200013 ◽

2004 ◽

Vol 47 (2) ◽

pp. 253-259 ◽

Cited By ~ 20

Author(s):

Samantha Salomão Caramori ◽

Claudinei Sousa Lima ◽

Kátia Flávia Fernandes

Keyword(s):

Enzymatic Activity ◽

Plant Species ◽

Biochemical Characterization ◽

Hymenaea Courbaril ◽

Annona Crassiflora

The aim of this work was to analyze and quantify the presence of antinutritional compounds such as lectins and trypsin-like inhibitors, polyphenols and tannins, and enzymatic activity of peroxidases and proteases in the seeds of Annona crassiflora Mart. (araticum), Hymenaea courbaril L. var. courbaril (jatobá), Plathymenia reticulata Benth. (vinhático), Zanthoxylum rhoifolium Lam. (maminha de porca), Apeiba tibourbou Aubl. (pau jangada), Salacia crassiflora Mart G. Don. (bacupari), and Sclerolobium paniculatum Vog. (carvoeiro). The results suggested that these plants could be used as new source of food.

Download Full-text