Transcriptomics of induced defense responses to greenbug aphid feeding in near isogenic wheat lines

AbstractPink stem borer (PSB) causes considerable yield losses to maize. Plant–insect interactions have significant implications for sustainable pest management. The present study demonstrated that PSB feeding, mechanical wounding, a combination of mechanical wounding and PSB regurgitation and exogenous application of methyl jasmonate have induced phenolic compound mediated defense responses both at short term (within 2 days of treatment) and long term (in 15 days of treatment) in leaf and stalk tissues of maize. The quantification of two major defense related phenolic compounds namely p-Coumaric acid (p-CA) and ferulic acid (FA) was carried out through ultra-fast liquid chromatography (UFLC) at 2 and 15 days after imposing the above treatments. The p-CA content induced in leaf tissues of maize genotypes were intrinsically higher when challenged by PSB attack at V3 and V6 stages in short- and long-term responses. Higher p-CA content was observed in stalk tissues upon wounding and regurgitation in short- and long-term responses at V3 and V6 stages. Significant accumulation of FA content was also observed in leaf tissues in response to PSB feeding at V3 stage in long-term response while at V6 stage it was observed both in short- and long-term responses. In stalk tissues, methyl jasmonate induced higher FA content in short-term response at V3 stage. However, at V6 stage PSB feeding induced FA accumulation in the short-term while, wounding and regurgitation treatment-induced defense responses in the long-term. In general, the resistant (DMRE 63, CM 500) and moderately resistant genotypes (WNZ ExoticPool) accumulated significantly higher contents of p-CA and FA content than susceptible ones (CM 202, BML 6) in most of the cases. The study indicates that phenolic mediated defense responses in maize are induced by PSB attack followed by wounding and regurgitation compared to the other induced treatments. Furthermore, the study confirmed that induced defense responses vary with plant genotype, stage of crop growth, plant tissue and short and long-term responses. The results of the study suggested that the Phenolic acids i.e. p-CA and FA may contribute to maize resistance mechanisms in the maize-PSB interaction system.

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Caterpillar salivary enzymes: "eliciting" a response

Phytoprotection ◽

10.7202/008904ar ◽

2004 ◽

Vol 85 (1) ◽

pp. 33-37 ◽

Cited By ~ 14

Author(s):

Magali Merkx-Jacques ◽

Jacqueline C. Bede

Keyword(s):

Artificial Diet ◽

Spodoptera Exigua ◽

Induced Defense ◽

Defense Responses ◽

Beet Armyworm ◽

Herbivorous Insect ◽

Plant Responses ◽

Plant Defense Responses ◽

Induced Defense Responses ◽

Bertha Armyworm

Abstract Plants exhibit remarkable plasticity in their ability to differentiate between herbivorous insect species and subtly adjust their defense responses to target distinct pests. One key mechanism used by plants to recognize herbivorous caterpillars is elicitors present in their oral secretions; however, these elicitors not only cause the induction of plant defenses but recent evidence suggests that they may also suppress plant responses. The absence of “expected changes” in induced defense responses of insect-infested plants has been attributed to hydrogen peroxide produced by caterpillar salivary glucose oxidase (GOX). Activity of this enzyme is variable among caterpillar species; it was detected in two generalist caterpillars, the beet armyworm (Spodoptera exigua) and the bertha armyworm (Mamestra configurata), but not in other generalist or specialist caterpillar species tested. In the beet armyworm, GOX activity fluctuated over larval development with high activity associated with the salivary glands of fourth instars. Larval salivary GOX activity of the beet armyworm and the bertha armyworm was observed to be significantly higher in caterpillars reared on artificial diet as compared with those reared on Medicago truncatula plants. This implies that a factor in the diet is involved in the regulation of caterpillar salivary enzyme activity. Therefore, plant diet may be regulating caterpillar oral elicitors that are involved in the regulation of plant defense responses: our goal is to understand these two processes.

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Citronella essential oil in the control and activation of coffee plants defense response against rust and brown eye spot

Ciência e Agrotecnologia ◽

10.1590/s1413-70542012000400001 ◽

2012 ◽

Vol 36 (4) ◽

pp. 383-390 ◽

Cited By ~ 5

Author(s):

Ricardo Borges Pereira ◽

Gilvaine Ciavareli Lucas ◽

Fabiano José Perina ◽

Pedro Martins Ribeiro Júnior ◽

Eduardo Alves

Keyword(s):

Essential Oil ◽

Induced Defense ◽

Defense Responses ◽

Plant Diseases ◽

Negative Effects ◽

Significant Control ◽

Citronella Oil ◽

Coffee Plants ◽

Induced Defense Responses ◽

Brown Eye Spot

The rust and brown eye spot are the main coffee diseases. The losses are due to intense defoliation of plants, which has reduced its production and longevity. The brown eye spot also occurs in fruits, with negative effects on the beverage quality. Some essential oils have presented promising results in the control of plant diseases, as an alternative to the use of fungicides. The objective of this study was to evaluate citronella essential oil in the control of rust and brown eye spot and in the activation of coffee plants defense responses. Twelve-month-old plants were sprayed with citronella oil 1000 µL L-1, acibenzolar-S-methyl 200 mg L-1 and tebuconazole fungicide 200 mg L-1. Plants were inoculated with Hemileia vastatrix and Cercospora coffeicola seven days later. The application was repeated after 30 days. Plants with five months were sprayed with the same treatments to assess the induced defense responses. Citronella oil controlled rust and brown eye spot with efficiencies of 47.2% and 29.7%, respectively, while tebuconazole presented control of 96.5% and 90.5%, respectively. Acibenzolar-S-methyl reduced brown eye spot by 55.9% and showed no significant control of rust. Citronella oil increased peroxidase and chitinase activities in five months coffee plants 336, and 24 and 336 hours after spraying, respectively. Acibenzolar-S-methyl increased peroxidase, chitinase and ββ-1,3-glucanase activities 192, 288 and 336; 24 and; 240 hours after spraying, respectively. The treatments did not increase accumulation of phenols, but a significant increase in lignin was observed in plants sprayed with citronella oil.

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Tomatidine and lycotetraose, hydrolysis products of α-tomatine byFusarium oxysporumtomatinase, suppress induced defense responses in tomato cells

FEBS Letters ◽

10.1016/j.febslet.2004.06.053 ◽

2004 ◽

Vol 571 (1-3) ◽

pp. 31-34 ◽

Cited By ~ 45

Author(s):

Shin-ichi Ito ◽

Tomomi Eto ◽

Shuhei Tanaka ◽

Naoki Yamauchi ◽

Hiroyuki Takahara ◽

...

Keyword(s):

Induced Defense ◽

Defense Responses ◽

Hydrolysis Products ◽

Induced Defense Responses

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Roles of systemic fungicide in antifungal activity and induced defense responses in rubber tree (Hevea brasiliensis) against leaf fall disease caused by Neopestalotiopsis cubana

Physiological and Molecular Plant Pathology ◽

10.1016/j.pmpp.2020.101511 ◽

2020 ◽

Vol 111 ◽

pp. 101511 ◽

Cited By ~ 3

Author(s):

Narit Thaochan ◽

Chaninun Pornsuriya ◽

Thanunchanok Chairin ◽

Anurag Sunpapao

Keyword(s):

Antifungal Activity ◽

Hevea Brasiliensis ◽

Induced Defense ◽

Rubber Tree ◽

Defense Responses ◽

Systemic Fungicide ◽

Leaf Fall ◽

Induced Defense Responses

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Delineating induced defense responses in wheat seedlings exposed to volatiles emitted by Puccinia triticina infected wheat

Physiological and Molecular Plant Pathology ◽

10.1016/j.pmpp.2020.101538 ◽

2020 ◽

Vol 112 ◽

pp. 101538 ◽

Cited By ~ 1

Author(s):

Mpho S. Mafa ◽

Howard D. Castelyn ◽

Gabre Kemp ◽

Botma Visser

Keyword(s):

Induced Defense ◽

Puccinia Triticina ◽

Defense Responses ◽

Wheat Seedlings ◽

Induced Defense Responses

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Regulation of Elicitor-Induced Defense Responses by Ca2+ Channels and the Cell Cycle in Tobacco BY-2 Cells

Tobacco BY-2 Cells: From Cellular Dynamics to Omics - Biotechnology in Agriculture and Forestry ◽

10.1007/3-540-32674-x_15 ◽

2006 ◽

pp. 207-221 ◽

Cited By ~ 7

Author(s):

Y. Kadota ◽

K. Kuchitsu

Keyword(s):

Cell Cycle ◽

Induced Defense ◽

Defense Responses ◽

Induced Defense Responses ◽

Ca2 Channels

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Aphid-Induced Defense Responses in Mi-1-Mediated Compatible and Incompatible Tomato Interactions

Molecular Plant-Microbe Interactions ◽

10.1094/mpmi.2003.16.8.699 ◽

2003 ◽

Vol 16 (8) ◽

pp. 699-708 ◽

Cited By ~ 158

Author(s):

Oscar Martinez de Ilarduya ◽

QiGuang Xie ◽

Isgouhi Kaloshian

Keyword(s):

Proteinase Inhibitors ◽

Induced Defense ◽

Defense Responses ◽

Root Knot Nematode ◽

Resistance Response ◽

Potato Aphid ◽

Ethylene Plant ◽

Aphid Feeding ◽

Transcript Profiles ◽

Incompatible Interactions

The tomato Mi-1 gene confers resistance to three species of root-knot nematode and potato aphid. We studied changes in expression of jasmonic acid (JA)- and salicylic acid (SA)- dependent defense genes in response to potato and green peach aphids. We determined changes in three PR proteins, lipoxygenase and proteinase inhibitors I and II transcripts, locally and systemically in both compatible and incompatible interactions in tomato. Transcripts for PR-1 were detected earlier and accumulated to higher levels in the incompatible than in the compatible potato aphid/tomato interactions. The transcript profiles of the other genes were similar in compatible compared with incompatible interactions. Pin1 and Pin2 RNAs were detected early and transiently in both compatible and incompatible interactions. In tomato plants containing Mi-1, systemic expression of PR-1 and GluB was detected in both compatible and incompatible interactions at 48 h after infestations with either aphid. These results suggest that aphid feeding involves both SA and JA/ethylene plant defense signaling pathways and that Mi-1-mediated resistance might involve a SA-dependent signaling pathway. Potato aphid feeding generated reactive oxygen species in both compatible and incompatible interactions. However, a hypersensitive response was absent in the Mi-1-mediated resistance response to potato aphids. Reciprocal grafting experiments revealed that resistance is cell autonomous, and local expression of Mi-1 is required for Mi-1-mediated resistance against the potato aphid.

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C-Terminal Region of Plant Ferredoxin-Like Protein Is Required to Enhance Resistance to Bacterial Disease in Arabidopsis thaliana

Phytopathology ◽

10.1094/phyto-08-10-0220 ◽

2011 ◽

Vol 101 (6) ◽

pp. 741-749 ◽

Cited By ~ 8

Author(s):

Yi-Hsien Lin ◽

Hsiang-En Huang ◽

Yen-Ru Chen ◽

Pei-Luan Liao ◽

Ching-Lian Chen ◽

...

Keyword(s):

Disease Resistance ◽

Transgenic Plants ◽

Nadph Oxidase ◽

Pseudomonas Syringae ◽

Induced Defense ◽

Defense Responses ◽

Bacterial Disease ◽

Oxidase Gene ◽

Induced Defense Responses ◽

Resistance To Ralstonia Solanacearum

Protein phosphorylation is an important biological process associated with elicitor-induced defense responses in plants. In a previous report, we described how plant ferredoxin-like protein (PFLP) in transgenic plants enhances resistance to bacterial pathogens associated with the hypersensitive response (HR). PFLP possesses a putative casein kinase II phosphorylation (CK2P) site at the C-terminal in which phosphorylation occurs rapidly during defense response. However, the contribution of this site to the enhancement of disease resistance and the intensity of HR has not been clearly demonstrated. In this study, we generated two versions of truncated PFLP, PEC (extant CK2P site) and PDC (deleted CK2P site), and assessed their ability to trigger HR through harpin (HrpZ) derived from Pseudomonas syringae as well as their resistance to Ralstonia solanacearum. In an infiltration assay of HrpZ, PEC intensified harpin-mediated HR; however, PDC negated this effect. Transgenic plants expressing these versions indicate that nonphosphorylated PFLP loses its ability to induce HR or enhance disease resistance against R. solanacearum. Interestingly, the CK2P site of PFLP is required to induce the expression of the NADPH oxidase gene, AtrbohD, which is a reactive oxygen species producing enzyme. This was further confirmed by evaluating the HR on NADPH oxidase in mutants of Arabidopsis. As a result, we have concluded that the CK2P site is required for the phosphorylation of PFLP to enhance disease resistance.

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