SNP discovery and high-density genetic mapping in faba bean (Vicia faba L.) permits identification of QTLs for ascochyta blight resistance

Plant Science ◽  
2014 ◽  
Vol 217-218 ◽  
pp. 47-55 ◽  
Author(s):  
Sukhjiwan Kaur ◽  
Rohan B.E. Kimber ◽  
Noel O.I. Cogan ◽  
Michael Materne ◽  
John W. Forster ◽  
...  
Keyword(s):  
Genetic Mapping ◽  
Vicia Faba ◽  
Faba Bean ◽  
Ascochyta Blight ◽  
High Density ◽  
Blight Resistance ◽  
Snp Discovery ◽  
Vicia Faba L

Isolate and organ-specific QTLs for ascochyta blight resistance in faba bean (Vicia faba L).

2003 ◽  
Vol 108 (6) ◽  
pp. 1071-1078 ◽  
Author(s):  
C. M. Avila ◽  
Z. Satovic ◽  
J. C. Sillero ◽  
D. Rubiales ◽  
M. T. Moreno ◽  
...  
Keyword(s):  
Vicia Faba ◽  
Faba Bean ◽  
Ascochyta Blight ◽  
Blight Resistance ◽  
Vicia Faba L ◽  
Organ Specific

QTLs for ascochyta blight resistance in faba bean (Vicia faba L.): validation in field and controlled conditions

2016 ◽  
Vol 67 (2) ◽  
pp. 216 ◽  
Author(s):  
S. G. Atienza ◽  
C. Palomino ◽  
N. Gutiérrez ◽  
C. M. Alfaro ◽  
D. Rubiales ◽  
...  
Keyword(s):  
Vicia Faba ◽  
Faba Bean ◽  
Ascochyta Blight ◽  
Field Conditions ◽  
Blight Resistance ◽  
Vicia Faba L ◽  
Chromosome Iii ◽  
Chromosome Ii ◽  
Ascochyta Fabae ◽  
Important Disease

Ascochyta blight is an important disease of faba bean (Vicia faba L.). Yield losses can be as high as 90% and losses of 35–40% are common. The line 29H is one of the most resistant accessions to the pathogen (Ascochyta fabae Speg.) ever described. In this work, we aimed to validate across generations the main quantitative trait loci (QTLs) for ascochyta blight resistance identified in the cross 29H × Vf136 and to test their stability under field conditions. QTLs located on chromosomes II and III have been consistently identified in the recombinant inbred line (RIL) population of this cross, in both controlled (growth chamber) and field conditions and, thus they are good targets for breeding. In addition, a new QTL for disease severity on pods has been located on chromosome VI, but in this case, further validation is still required. A synteny-based approach was used to compare our results with previous QTL works dealing with this pathogen. Our results suggest that the QTL located on chromosome II, named Af2, is the same one reported by other researchers, although it is likely that the donors of resistance differ in the allele conferring the resistance. By contrast, the location of Af3 on chromosome III does not overlap with the position of Af1 reported by other authors, suggesting that Af3 may be an additional source of resistance to ascochyta blight.

scholarly journals Effect of Some Agronomic Practices on Ascochyta Blight Severity and Yield of Faba Bean (Vicia faba L.) in Tunisia

2021 ◽  
Author(s):  
A. Ouji ◽  
S. Chekali ◽  
M. Rouaissi
Keyword(s):  
Vicia Faba ◽  
Faba Bean ◽  
Ascochyta Blight ◽  
Growth And Yield ◽  
Row Spacing ◽  
Seed Rate ◽  
Agronomic Practices ◽  
Vicia Faba L ◽  
Cropping Seasons ◽  
Effect On Yield

Background: In Tunisia, faba bean (Vicia faba L.) is the first major food legume. The development of faba bean production is facing several biotic constraints. Faba bean Ascochyta blight caused by Ascochyta fabae is one of the most destructive diseases of faba bean and can cause significant yield loss under favorable conditions. As only incomplete resistance ABL varieties are available, some agronomic practices should be applied to control and reduce Ascochyta blight incidence wherever possible. Therefore, this work was undertaken to evaluate the effect of spacing row and seed rate on ABL severity, growth and yield of faba bean. Methods: A split-plot design with three replications was adopted to carry out this study during 2018 and 2019 cropping seasons. ‘Bachaar’ faba bean variety was sown at 40 and 60 cm row spacing and at three seed rates (100, 140 and 200 kg ha-1). ABL severity was assessed visually on a 0-9 scale and agro-morphological traits were measured. Analysis of variance was used to analyze the data. Correlations between agronomic traits, row spacing, seed rate and ABL severity were investigated. Result: Results showed that seed rate has a larger effect on yield than row spacing. In both cropping seasons, the highest grain yield was recorded in 60 cm row spacing and 140 kg ha-1 seed rate treatment. So, this treatment is recommended for obtaining high yield of faba bean. Most of the variation in disease severity was associated with seed rate (r=0.62). The highest ABL score severity was noted at 200 kg ha-1 rate. Over both years, wide row spacing and low seed rate reduced ABL severity. In this study, the small amounts of ABL disease (which reached a score of 5.3 and 4.7 in 2018 and 2019, respectively) had little or no effect on yield.

Confirmation of QTLs controlling Ascochyta fabae resistance in different generations of faba bean (Vicia faba L.)

2009 ◽  
Vol 60 (4) ◽  
pp. 353 ◽  
Author(s):  
R. Díaz-Ruiz ◽  
Z. Satovic ◽  
C. M. Ávila ◽  
C. M. Alfaro ◽  
M. V. Gutierrez ◽  
...  
Keyword(s):  
Vicia Faba ◽  
Disease Severity ◽  
Faba Bean ◽  
Recombinant Inbred ◽  
Recombinant Inbred Lines ◽  
Ascochyta Blight ◽  
Inbred Lines ◽  
Phenotypic Variance ◽  
Vicia Faba L ◽  
Ascochyta Fabae

Ascochyta blight, caused by Ascochyta fabae Speg., is a disease of faba bean (Vicia faba L.) of worldwide distribution. In this study we have conducted an experiment on Ascochyta fabae resistance in 165 recombinant inbred lines (RILs) developed by single-seed descent from the cross between resistant and susceptible lines (Vf6 × Vf136) in which A. fabae resistance QTLs (quantitative trait loci) have been previously reported in the original F2 population. Recombinant inbred lines were inoculated under controlled growth chamber conditions and evaluated for disease severity and infection type index. The linkage map was constructed by MAPMAKER V2.0 and the QTL analysis was carried out using QTL Cartographer. Two hundred and seventy-seven markers (238 RAPDs, 4 isozymes, 5 ESTs, 1 SCAR, 6 SSRs, 2 STSs, and 21 intron-spanning markers) mapped into 21 linkage groups covering 2.856.7 cM, with a mean inter-marker distance of 12.72 cM. Composite interval mapping identified two zones of putative QTL action in the RIL population for DSL (disease severity on leaves) and DSS (disease severity on stems) traits. Putative QTLs (Af1 and Af2) were identified on chromosome 3 and chromosome 2, respectively, and jointly explained 24% of the phenotypic variance of DSL and 16% of DSS. With this study we have (1) confirmed the QTLs for ascochyta blight resistance found in F3 families in the derived RILs (F6), (2) re-estimated their position and genetic effects, and (3) assessed the stability of these QTLs in different genetic backgrounds by comparison of the mapping data with a previous QTL study.

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