Confirmation of QTLs controlling Ascochyta fabae resistance in different generations of faba bean (Vicia faba L.)

2009 ◽  
Vol 60 (4) ◽  
pp. 353 ◽  
Author(s):  
R. Díaz-Ruiz ◽  
Z. Satovic ◽  
C. M. Ávila ◽  
C. M. Alfaro ◽  
M. V. Gutierrez ◽  
...  
Keyword(s):  
Vicia Faba ◽  
Disease Severity ◽  
Faba Bean ◽  
Recombinant Inbred ◽  
Recombinant Inbred Lines ◽  
Ascochyta Blight ◽  
Inbred Lines ◽  
Phenotypic Variance ◽  
Vicia Faba L ◽  
Ascochyta Fabae

Ascochyta blight, caused by Ascochyta fabae Speg., is a disease of faba bean (Vicia faba L.) of worldwide distribution. In this study we have conducted an experiment on Ascochyta fabae resistance in 165 recombinant inbred lines (RILs) developed by single-seed descent from the cross between resistant and susceptible lines (Vf6 × Vf136) in which A. fabae resistance QTLs (quantitative trait loci) have been previously reported in the original F2 population. Recombinant inbred lines were inoculated under controlled growth chamber conditions and evaluated for disease severity and infection type index. The linkage map was constructed by MAPMAKER V2.0 and the QTL analysis was carried out using QTL Cartographer. Two hundred and seventy-seven markers (238 RAPDs, 4 isozymes, 5 ESTs, 1 SCAR, 6 SSRs, 2 STSs, and 21 intron-spanning markers) mapped into 21 linkage groups covering 2.856.7 cM, with a mean inter-marker distance of 12.72 cM. Composite interval mapping identified two zones of putative QTL action in the RIL population for DSL (disease severity on leaves) and DSS (disease severity on stems) traits. Putative QTLs (Af1 and Af2) were identified on chromosome 3 and chromosome 2, respectively, and jointly explained 24% of the phenotypic variance of DSL and 16% of DSS. With this study we have (1) confirmed the QTLs for ascochyta blight resistance found in F3 families in the derived RILs (F6), (2) re-estimated their position and genetic effects, and (3) assessed the stability of these QTLs in different genetic backgrounds by comparison of the mapping data with a previous QTL study.

QTLs for ascochyta blight resistance in faba bean (Vicia faba L.): validation in field and controlled conditions

2016 ◽  
Vol 67 (2) ◽  
pp. 216 ◽  
Author(s):  
S. G. Atienza ◽  
C. Palomino ◽  
N. Gutiérrez ◽  
C. M. Alfaro ◽  
D. Rubiales ◽  
...  
Keyword(s):  
Vicia Faba ◽  
Faba Bean ◽  
Ascochyta Blight ◽  
Field Conditions ◽  
Blight Resistance ◽  
Vicia Faba L ◽  
Chromosome Iii ◽  
Chromosome Ii ◽  
Ascochyta Fabae ◽  
Important Disease

Ascochyta blight is an important disease of faba bean (Vicia faba L.). Yield losses can be as high as 90% and losses of 35–40% are common. The line 29H is one of the most resistant accessions to the pathogen (Ascochyta fabae Speg.) ever described. In this work, we aimed to validate across generations the main quantitative trait loci (QTLs) for ascochyta blight resistance identified in the cross 29H × Vf136 and to test their stability under field conditions. QTLs located on chromosomes II and III have been consistently identified in the recombinant inbred line (RIL) population of this cross, in both controlled (growth chamber) and field conditions and, thus they are good targets for breeding. In addition, a new QTL for disease severity on pods has been located on chromosome VI, but in this case, further validation is still required. A synteny-based approach was used to compare our results with previous QTL works dealing with this pathogen. Our results suggest that the QTL located on chromosome II, named Af2, is the same one reported by other researchers, although it is likely that the donors of resistance differ in the allele conferring the resistance. By contrast, the location of Af3 on chromosome III does not overlap with the position of Af1 reported by other authors, suggesting that Af3 may be an additional source of resistance to ascochyta blight.

Isolate and organ-specific QTLs for ascochyta blight resistance in faba bean (Vicia faba L).

2003 ◽  
Vol 108 (6) ◽  
pp. 1071-1078 ◽  
Author(s):  
C. M. Avila ◽  
Z. Satovic ◽  
J. C. Sillero ◽  
D. Rubiales ◽  
M. T. Moreno ◽  
...  
Keyword(s):  
Vicia Faba ◽  
Faba Bean ◽  
Ascochyta Blight ◽  
Blight Resistance ◽  
Vicia Faba L ◽  
Organ Specific

scholarly journals Effect of Some Agronomic Practices on Ascochyta Blight Severity and Yield of Faba Bean (Vicia faba L.) in Tunisia

2021 ◽  
Author(s):  
A. Ouji ◽  
S. Chekali ◽  
M. Rouaissi
Keyword(s):  
Vicia Faba ◽  
Faba Bean ◽  
Ascochyta Blight ◽  
Growth And Yield ◽  
Row Spacing ◽  
Seed Rate ◽  
Agronomic Practices ◽  
Vicia Faba L ◽  
Cropping Seasons ◽  
Effect On Yield

Background: In Tunisia, faba bean (Vicia faba L.) is the first major food legume. The development of faba bean production is facing several biotic constraints. Faba bean Ascochyta blight caused by Ascochyta fabae is one of the most destructive diseases of faba bean and can cause significant yield loss under favorable conditions. As only incomplete resistance ABL varieties are available, some agronomic practices should be applied to control and reduce Ascochyta blight incidence wherever possible. Therefore, this work was undertaken to evaluate the effect of spacing row and seed rate on ABL severity, growth and yield of faba bean. Methods: A split-plot design with three replications was adopted to carry out this study during 2018 and 2019 cropping seasons. ‘Bachaar’ faba bean variety was sown at 40 and 60 cm row spacing and at three seed rates (100, 140 and 200 kg ha-1). ABL severity was assessed visually on a 0-9 scale and agro-morphological traits were measured. Analysis of variance was used to analyze the data. Correlations between agronomic traits, row spacing, seed rate and ABL severity were investigated. Result: Results showed that seed rate has a larger effect on yield than row spacing. In both cropping seasons, the highest grain yield was recorded in 60 cm row spacing and 140 kg ha-1 seed rate treatment. So, this treatment is recommended for obtaining high yield of faba bean. Most of the variation in disease severity was associated with seed rate (r=0.62). The highest ABL score severity was noted at 200 kg ha-1 rate. Over both years, wide row spacing and low seed rate reduced ABL severity. In this study, the small amounts of ABL disease (which reached a score of 5.3 and 4.7 in 2018 and 2019, respectively) had little or no effect on yield.

SNP discovery and high-density genetic mapping in faba bean (Vicia faba L.) permits identification of QTLs for ascochyta blight resistance

Plant Science ◽  
2014 ◽  
Vol 217-218 ◽  
pp. 47-55 ◽  
Author(s):  
Sukhjiwan Kaur ◽  
Rohan B.E. Kimber ◽  
Noel O.I. Cogan ◽  
Michael Materne ◽  
John W. Forster ◽  
...  
Keyword(s):  
Genetic Mapping ◽  
Vicia Faba ◽  
Faba Bean ◽  
Ascochyta Blight ◽  
High Density ◽  
Blight Resistance ◽  
Snp Discovery ◽  
Vicia Faba L

Locating genes associated with Ascochyta fabae resistance in Vicia faba

2003 ◽  
Vol 54 (1) ◽  
pp. 85 ◽  
Author(s):  
B. Román* ◽  
Z. Satovic* ◽  
C. M. Avila ◽  
D. Rubiales ◽  
M. T. Moreno ◽  
...  
Keyword(s):  
Vicia Faba ◽  
Infection Type ◽  
Ascochyta Blight ◽  
Interval Mapping ◽  
Gene Location ◽  
Phenotypic Variance ◽  
Chromosome 2 ◽  
Linkage Groups ◽  
Sources Of Resistance ◽  
Ascochyta Fabae

Ascochyta blight, caused by Ascochyta fabae Speg.,is a disease of faba bean (Vicia faba L.) of world-wide distribution. Although sources of resistance have been identified, little is known about its genetic basis and gene location. Molecular analyses were carried out using plant tissue from 196 individual F2 plants derived from a cross between susceptible and resistant lines. F2-derived F3 lines were inoculated under controlled growth-chamber conditions and evaluated for disease severity and infection type index. The linkage map was constructed by MAPMAKER V2.0 and the quantitative trait loci (QTL) analysis was carried out using QTL Cartographer. One hundred and twenty-one out of 130 marker loci could be mapped into 16 linkage groups. Nine linkage groups were assigned to specific chromosomes analysing common markers with previous studies that used primary trisomics to establish the chromosomal location of markers/genes. By simple interval mapping (SIM), 2 zones of putative QTL action were detected. Using forward-backward stepwise regression, 4 markers were selected to be used as cofactors in composite interval mapping (CIM). Putative QTLs (Af1 and Af2) were identified on linkage groups VIII (chromosome 3) and IVa (chromosome 2), respectively, and jointly contributed about 46% of the total phenotypic variance. Af1 displayed pure additive gene action and Af2 showed considerable dominant effect.

scholarly journals Large-Scale Transcriptome Analysis in Faba Bean (Vicia faba L.) under Ascochyta fabae Infection

PLoS ONE ◽  
2015 ◽  
Vol 10 (8) ◽  
pp. e0135143 ◽  
Author(s):  
Sara Ocaña ◽  
Pedro Seoane ◽  
Rocio Bautista ◽  
Carmen Palomino ◽  
Gonzalo M. Claros ◽  
...  
Keyword(s):  
Vicia Faba ◽  
Faba Bean ◽  
Transcriptome Analysis ◽  
Large Scale ◽  
Vicia Faba L ◽  
Ascochyta Fabae

scholarly journals Solanum habrochaites Accession LA1777 Recombinant Inbred Lines Are Not Resistant to Tomato Yellow Leaf Curl Virus or Tomato Mottle Virus

HortScience ◽  
2007 ◽  
Vol 42 (5) ◽  
pp. 1149-1152 ◽  
Author(s):  
Aliya Momotaz ◽  
John W. Scott ◽  
David J. Schuster
Keyword(s):  
Disease Severity ◽  
Recombinant Inbred ◽  
Recombinant Inbred Lines ◽  
Inbred Lines ◽  
Tomato Yellow Leaf ◽  
Mottle Virus ◽  
Yellow Leaf ◽  
Ril Population ◽  
Leaf Curl Virus ◽  
Leaf Curl

Cultivated tomato (Solanum lycopersicum L.) accessions have been susceptible to the whitefly-transmitted begomoviruses Tomato yellow leaf curl virus (TYLCV) and Tomato mottle virus (ToMoV) that can cause serious crop damage. S. habrochaites accession LA1777 has been reported to be resistant to TYLCV. To locate putative virus resistance genes, 89 recombinant inbred lines (RILs) previously developed from LA1777 in a tomato background, LA1777 and the susceptible RIL parent E6203, were screened against the begomoviruses TYLCV and ToMoV. An initial study showed 18 RILs had less disease severity to TYLCV or ToMoV. Eight RILs had S. habrochaites alleles at TG27 (restriction fragment length polymorphism marker) on chromosome 1, three RILs had S. habrochaites alleles at TG202 on chromosome 7, and one RIL had S. habrochaites alleles at both marker loci. The RILs with these regions were intercrossed in 10 different cross combinations and F2 seeds were then obtained. The F2 progenies were inoculated separately with both viruses and then evaluated in the field. The F2 plants with less disease severity were selected, but most did not have the markers from the hypothetical resistance regions. The F3 progenies were then inoculated and rated for disease severity to both viruses. None of the F3s demonstrated any increased level of resistance, even if derived from F2s homozygous for the target regions from both chromosomes. All plants from every cross combination were susceptible for both TYLCV and ToMoV, suggesting that there is no begomovirus resistance in the LA1777 RIL population. Some limitations of capturing all genes in an RIL population derived from an outcrossing accession are discussed.

EFFECTIVENESS OF SEED AND FOLIAR APPLICATION OF FUNGICIDES TO CONTROL ASCOCHYTA BLIGHT OF FABABEANS

1979 ◽  
Vol 59 (3) ◽  
pp. 661-666 ◽  
Author(s):  
P. D. KHARBANDA ◽  
C. C. BERNIER
Keyword(s):  
Vicia Faba ◽  
Foliar Application ◽  
Ascochyta Blight ◽  
Field Tests ◽  
Seed Infection ◽  
Seed Treatments ◽  
Systemic Fungicides ◽  
Vicia Faba L ◽  
Ascochyta Fabae ◽  
Seedling Infection

Control of blight (Ascochyta fabae Speg.) of fababeans (Vicia faba L.) was attempted by using systemic and non-systemic fungicides in laboratory and field tests during 1974–1976. In the laboratory assays, all seed treatments containing the systemic fungicides benomyl or thiabendazole effectively controlled seed-borne Ascochyta but only soaking seeds in benomyl-thiram (0.2% a.i.) for 8 h effected complete eradication. In the field tests none of the seed treatments significantly reduced the seedling infection. When evaluated as foliar sprays, chlorothalonil was the most promising and was evaluated further on V. faba cultivars Erfordia and Ackerperle. One spray of this fungicide (0.1% a.i.) significantly reduced the disease severity in both cultivars. Seed infection was prevented only in Ackerperle by a minimum of two sprays 3 wk apart. In untreated plots, the disease was more severe in Erfordia than in Ackerperle.

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