A novel R3 MYB transcriptional repressor, MaMYBx, finely regulates anthocyanin biosynthesis in grape hyacinth

Plant Science ◽  
2020 ◽  
Vol 298 ◽  
pp. 110588
Author(s):  
Han Zhang ◽  
Jiaxin Gong ◽  
Kaili Chen ◽  
Wenkong Yao ◽  
Boxiao Zhang ◽  
...  
Keyword(s):  
Anthocyanin Biosynthesis ◽  
Transcriptional Repressor ◽  
Grape Hyacinth

scholarly journals A R2R3-MYB Transcription Factor, VvMYBC2L2, Functions as a Transcriptional Repressor of Anthocyanin Biosynthesis in Grapevine (Vitis vinifera L.)

Molecules ◽  
2018 ◽  
Vol 24 (1) ◽  
pp. 92 ◽  
Author(s):  
Ziguo Zhu ◽  
Guirong Li ◽  
Li Liu ◽  
Qingtian Zhang ◽  
Zhen Han ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Vitis Vinifera ◽  
Anthocyanin Biosynthesis ◽  
Transcriptional Repressor ◽  
Negative Regulator ◽  
Myb Transcription Factor ◽  
Vitis Vinifera L ◽  
Flavonoid Pathway ◽  
Leucoanthocyanidin Reductase ◽  
R2r3 Myb

In grapevine, the MYB transcription factors play an important role in the flavonoid pathway. Here, a R2R3-MYB transcription factor, VvMYBC2L2, isolated from Vitis vinifera cultivar Yatomi Rose, may be involved in anthocyanin biosynthesis as a transcriptional repressor. VvMYBC2L2 was shown to be a nuclear protein. The gene was shown to be strongly expressed in root, flower and seed tissue, but weakly expressed during the fruit development in grapevine. Overexpressing the VvMYBC2L2 gene in tobacco resulted in a very marked decrease in petal anthocyanin concentration. Expression analysis of flavonoid biosynthesis structural genes revealed that chalcone synthase (CHS), dihydroflavonol 4-reductase (DFR), leucoanthocyanidin reductase (LAR) and UDP glucose flavonoid 3-O-glucosyl transferase (UFGT) were strongly down-regulated in the VvMYBC2L2-overexpressed tobacco. In addition, transcription of the regulatory genes AN1a and AN1b was completely suppressed in transgenic plants. These results suggested that VvMYBC2L2 plays a role as a negative regulator of anthocyanin biosynthesis.

scholarly journals Prospects for Knockout of MYB60, a Transcriptional Repressor of Anthocyanin Biosynthesis, in Brassicaceae Plants by Genome Editing

2022 ◽  
Author(s):  
Elena Mikhaylova ◽  
Alexander Artyukhin ◽  
Michael Shein ◽  
Khalit Musin ◽  
Anna Sukhareva ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Genome Editing ◽  
Agronomic Traits ◽  
Anthocyanin Biosynthesis ◽  
Gene Knockout ◽  
Transcriptional Repressor ◽  
Academic Community ◽  
Plant Responses ◽  
Individual Plant ◽  
Brassica Napus L

The Brassicaceae plant family contains many economically important crops such as Brassica napus L., Brassica rapa L., Brassica oleracea L., Brassica juncea L., Eruca sativa Mill., Camelina sativa L. and Raphanus sativus L. Insufficient data on the genetic regulation of agronomic traits in these species complicates the editing of their genomes. In recent years, the attention of the academic community has been drawn to anthocyanin hyperaccumulation. This trait is not only beneficial for human health, but can also increase plant resistance to stress. MYB transcription factors are the main regulators of flavonoid biosynthesis in plants. Some of them are well studied in Arabidopsis thaliana. The AtMYB60 gene is a transcriptional repressor of anthocyanin biosynthesis, and it also negatively impacts plant responses to drought stress. Myb60 is one of the least studied transcription factors with similar functions in Brassicaceae. There is a high degree of homology between predicted MYB60 genes of A. thaliana and related plant species. However, functions of these homologous genes have never been studied. Gene knockout by CRISPR/Cas technology remains the easiest way to perform genome editing in order to discover the role of individual plant genes. Disruption of genes acting as negative regulators of anthocyanin biosynthesis could result in color staining of plant tissues and an increase in stress tolerance. In the present study, we investigated the AtMYB60 gene and its homologs in Brassicaceae plants and suggested universal gRNAs to knockout these genes. Keywords: CRISPR, Brassicaceae, MYB60, knockout, anthocyanin

scholarly journals Identification and Functional Analysis of a Flavonol Synthase Gene from Grape Hyacinth

Molecules ◽  
2019 ◽  
Vol 24 (8) ◽  
pp. 1579 ◽  
Author(s):  
Hongli Liu ◽  
Beibei Su ◽  
Han Zhang ◽  
Jiaxin Gong ◽  
Boxiao Zhang ◽  
...  
Keyword(s):  
Anthocyanin Biosynthesis ◽  
Expression Patterns ◽  
Flower Color ◽  
Pcr Analysis ◽  
Anthocyanin Content ◽  
In Planta ◽  
Total Anthocyanin ◽  
Flavonol Synthase ◽  
Total Anthocyanin Content ◽  
Grape Hyacinth

Flavonols are important copigments that affect flower petal coloration. Flavonol synthase (FLS) catalyzes the conversion of dihydroflavonols to flavonols. In this study, we identified a FLS gene, MaFLS, expressed in petals of the ornamental monocot Muscari aucheri (grape hyacinth) and analyzed its spatial and temporal expression patterns. qRT-PCR analysis showed that MaFLS was predominantly expressed in the early stages of flower development. We next analyzed the in planta functions of MaFLS. Heterologous expression of MaFLS in Nicotiana tabacum (tobacco) resulted in a reduction in pigmentation in the petals, substantially inhibiting the expression of endogenous tobacco genes involved in anthocyanin biosynthesis (i.e., NtDFR, NtANS, and NtAN2) and upregulating the expression of NtFLS. The total anthocyanin content in the petals of the transformed tobacco plants was dramatically reduced, whereas the total flavonol content was increased. Our study suggests that MaFLS plays a key role in flavonol biosynthesis and flower coloration in grape hyacinth. Moreover, MaFLS may represent a new potential gene for molecular breeding of flower color modification and provide a basis for analyzing the effects of copigmentation on flower coloration in grape hyacinth.

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