Roles of CsBRC1-like in leaf and lateral branch development in cucumber

Plant Science ◽  
2021 ◽  
Vol 302 ◽  
pp. 110681
Author(s):  
Junjun Shen ◽  
Danfeng Ge ◽  
Xiaofei Song ◽  
Jiajing Xiao ◽  
Xiaofeng Liu ◽  
...  
2021 ◽  
Author(s):  
Junling Dou ◽  
Huihui Yang ◽  
Dongling Sun ◽  
Sen Yang ◽  
Shouru Sun ◽  
...  

Abstract Lateral branching is one of the most important traits, which directly determines plant 27 architecture and crop productivity. Commercial watermelon has the characteristics of multiple 28 lateral branches, and it is time-consuming and labor costing to manually remove the lateral 29 branches in traditional watermelon cultivation. In our present study, a lateral branchless trait was 30 identified in watermelon material W CZ, and genetic analysis revealed that it was controlled by a 31 single recessive gene, which named as Clbl . A bulked segregant sequencing (BSA seq) and 32 linkage analysis was conducted to primarily mapping of Clbl on watermelon chromosome 4 33 Next-generation sequencing aided marker discovery and a large mapping population consisting of 34 1406 F 2 plants was used to further mapped the Clbl locus into a 9011 bp candidate region which 35 harbored only one candidate gene Cla018392 encoding a TERMINAL FLOWER 1 gene. Sequence 36 comparison of Cla018392 between two parental lines revealed that there was a SNP detected from 37 C to A in the coding region in the branchless inbred line WCZ , which resulted in a mutation of 38 Alanine (GCA) to Glutamate (GAA) at the fourth exon A dCAPS marker was developed from the 39 SNP locus, which was co-segregated with the branchless phenotype in both BC 1 and F 2 population, 40 and it was also further validated in 152 natural watermelon accessions. qRT PCR and in situ 41 hybridization showed that the expression levels of Cla0 18392 was significantly reduced in the 42 axillary bud and apical bud in the branchless line WCZ Ectopic expression of ClTFL1 in 43 Arabidopsis showed an increased number of lateral branches. The results of this study will be 44 useful for better understanding the molecular mechanism of lateral branch development in 45 watermelon and for the development of marker-assisted selection (MAS) for new branchless 46 watermelon cultivars.


HortScience ◽  
1992 ◽  
Vol 27 (1) ◽  
pp. 30-32 ◽  
Author(s):  
David W. Burger ◽  
Pavel Svihra ◽  
Richard Harris

Treeshelters were used for the nursery production of Cedrus deodara Loud. (deodar cedar), Quercus ilex L. (holly oak), and Magnolia grandiflora L. (southern magnolia) trees growing in 19-liter containers. Air temperature, relative humidity, and CO, concentration were higher inside the treeshelters than outside. Trees grown inside treeshelters were 74% to 174% taller than trees grown without shelters. Trunk caliper of Magnolia and Quercus was not affected, however, for Cedrus trees caliper was larger for trees grown without a shelter. Upon removal of the shelter, Cedrus trees were incapable of supporting their own weight. Lateral branch development was inhibited and leaf senescence was greater with Magnolia trees grown in a shelter. Quercus trees grown in shelters were ready to be transplanted into the landscape. Water use was similar for trees grown with or without shelters. Trees grown in shelters had lower root fresh weights.


HortScience ◽  
2007 ◽  
Vol 42 (2) ◽  
pp. 251-256 ◽  
Author(s):  
Don C. Elfving ◽  
Dwayne B. Visser

Improving lateral branch development in young sweet cherry trees without reliance on pruning is a desirable component of tree training programs, especially for high-density systems. Applications of two proprietary formulations of 6-benzyladenine and gibberellins A4 and A7 (Promalin, Valent Biosciences, Walnut Creek, Calif.; and Perlan, Fine Americas, Walnut Creek, Calif.) to individual buds or intact bark of unpruned sweet cherry central leader shoots at green-tip had little effect on lateral shoot growth from buds or on distribution of new shoot growth along the treated leader shoots. Scoring, nicking, or notching cuts alone also had inconsistent effects on shoot development and distribution. In some trials, bud removal (or disbudding, removing every fourth bud on 1-year-old shoots) produced limited improvement of lateral shoot development and vertical distribution. Combining nicking, notching, scoring, or bark scraping with the application of cytokinin–gibberellic acid solution to the cut area greatly improved both number of shoots developed and the numbers originating from the lower portions of treated leader shoots. Removing the physical barrier to bioregulator product contact with active tissues was a primary factor in improving treatment efficacy.


2006 ◽  
Vol 46 (5) ◽  
pp. 707 ◽  
Author(s):  
P. Sansberro ◽  
L. Mroginski ◽  
R. Bottini

Intensive yerba mate (Ilex paraguariensis St. Hil.) orchard management is needed to improve productivity. Full size trees with strong apical dominance are being replaced by smaller, more efficient trees, planted at higher densities for mechanical harvest. The production potential of a high-density field is maximised when cultural methods that control tree size and induce a significant number of well-distributed branches are used. The application of chemical pruning agents to shape the architecture of the seedling may be a viable alternative to induce lateral shoot development in replacement of the hand pruning technique. Nursery grown yerba mate seedlings were treated with a single foliar spray of aqueous solutions containing either benzyladenine (BA), 2,3:4,6-di-o-isopropylidene-2-keto-L-gulonic acid sodium salt (dikegulac) or 2,3,5-triiodobenzoic acid (TIBA), at the beginning of the second flash of growth (summer). A hand-pruned treatment was also included. BA-treated plants produced more new shoots than untreated controls or plants that received other treatments. BA treatment at 8.8 mmol/L increased the number of branches to up to 8 branches per single stem. Control plants sprayed with distilled water did not branch, while hand-pruned plants rarely showed more than 1 branch. Dikegulac was less effective than BA at promoting lateral branch development and its phytotoxicity was concentration dependent. TIBA did not stimulate budbreak; in addition, primary shoot development was delayed and leaf indentations were apparent. The results suggest that BA can be used to obtain high quality multiply-branched yerba mate plants.


1976 ◽  
Vol 12 (4) ◽  
pp. 401-410 ◽  
Author(s):  
W. Erskine ◽  
T. N. Khan

SUMMARYThe effects of populations of 33,000–250,000 plants/ha on growth and grain yield of two cowpea genotypes of bush growth habit was studied in lowland Papua New Guinea. The optimum economic seed rate seems to be 100,000 plants/ha; increasing the plant population decreased pods/plant and lateral branch development, but increased seed number/pod. Genotype UV.53 yielded more than UV.19 due to a combination of all three yield components, and a large dry weight. Growth analysis showed that treatment effects were mediated by differences in growth between 8 weeks and harvest.


HortScience ◽  
1997 ◽  
Vol 32 (4) ◽  
pp. 690-693 ◽  
Author(s):  
Michelle L. Bell ◽  
Roy A. Larson ◽  
Douglas A. Bailey

Dikegulac, dikegulac + GA4+7, BA, and Promalin (GA4+7 + BA) were evaluated as lateral shoot-inducing agents on greenhouse forcing azalea, Rhododendron cultivars Gloria and Prize. The addition of GA4+7 (1000 or 2000 mg.L-1) to a commercial rate of dikegulac (3900 mg.L-1) did not effectively increase plant diameter or leaf width compared to plants sprayed with dikegulac alone. The combination of dikegulac and GA4+7 (3900 + 2000 mg.L-1, respectively) was more phytotoxic than dikegulac alone. Foliar sprays of BA and Promalin at 1000 and 2000 mg.L-1 and 1000 and 1816 mg.L-1, respectively, did not increase lateral shoot count. Neither the addition of GA4+7 to dikegulac nor the use of Promalin is a viable alternative to dikegulac application for inducing lateral branch development of dikegulac-sensitive cultivars. Chemical names used: Na 2,3:4,6-Bis-0-(l-methylethylidene)-α-L-xylo-2-hexulofuranosonic acid (dikegulac), (lα,2β,4aα,4bβ,10β)-2,4a,7-trihydroxy-l-methyl-8-methylenegibb-3-ene-l,10-dicarboxylic acid l,4a-lactone (GA4+7),N-(phenylmethyl)-lH-purin-6-amine (BA), and Promalin [1:1 (wt/wt) GA4+7 and BA].


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