PENGARUH EKSTRAK KASAR BUAH PALA MYRISTICA ARGENTEA TERHADAP PERTUMBUHAN IKAN KOI CYPRINUS CAPRIO

Ikan koi di Indonesia merupakan ikan hias favorit dan banyak digemari masyarakat luas karena tubuhnya yang mempesona dan harganya relatif tidak terlalu mahal. Ikan koi sekarang ini masih menjadi salah satu komoditas perdagangan yang cukup baik dalam bidang perikanan. Tujuan dari penelitian ini adalah untuk mengetahui Pengaruh Ekstrak Kasar Buah Pala Myristica Argentea Terhadap Pertumbuhan Ikan Koi Cyprinus Caprio. Metode penelitian yang digunakan adalah Rancangan Acak Lengkap (RAL) ,dengan empat perlakuan dan tiga ulangan yaitu perlakuan A (Dosis 5% ekstrak kasar buah pala), perlakuan B (Dosis 10% ekstrak kasar buah pala), perlakuan C (Dosis 15% ekstrak kasar buah pala) dan perlakuan D ( Dosis 0 % ekstrak kasar buah pala). Parameter uji berupa lajui pertumhuhan bobot dan pertumbuhan panjang. Hasil penelitian menunjukkan bahwa ekstrak Kasar Daging Buah Pala myristica argentea memberikan pengaruh yang baik terhadap pertumbuhan ikan ko setelah diberikan perlakuan.. Dosis yang baik untuk pertumbuhan berat dan panjang ikan koi ialah dosis 10% - 5%. Koi fish in Indonesia is a favorite ornamental fish and much-loved by the wider community because of its charming body and relatively inexpensive price. Koi fish is still one of the trade commodities that is quite good in the field of fisheries. The purpose of this study was to determine the Effect of Crude Extract of Nutmeg Myristica Argentea on the Growth of Cyprinus Caprio Koi Fish. The research method used was a completely randomized design (CRD), with four treatments and three replications, namely treatment A (5% dose of nutmeg crude extract), treatment B (10% dose of nutmeg crude extract), treatment C (15% dose of nutmeg extract). crude nutmeg) and treatment D (Dose of 0% crude extract of nutmeg). Test parameters in the form of weight growth rate and length growth. The results showed that the crude extract of the Nutmeg myristica argentea had a good effect on the growth of koi fish after being given treatment. A good dose for the growth of weight and length of koi fish is a dose of 10% - 5%

ADDITION OF PUMPKIN FLOUR (Cucurbita moschata D.)AND PAPAYA FLOUR(Carica papaya)TO FEED TO INCREASE THE BRIGHTNESS COLOR OF THE GOLDFISH (Carrasius auratus)

The purpose of this study was to analyze the effect of the addition of pumpkin flour (C. moschata D.) and papaya flour (C. papaya) to feed to increase the brightness of the gold fish (C. auratus) color. The method used is an experimental method with a completely randomized design, consisting of four treatments and three replications of P0 0% (Control), P1 10% , P2 20%, P3 30%. Research parameters include carotenoid analysis, hunter’s color test, absolute weight growth, absolute specific growth, absolute length growth , FCR, EPP, survival rate and water quality. The results showed that the addition of a mixture of pumpkin flour and papaya flour in the goldfish can affect the value of feed conversion, feed afficiency, survival rate, carotenoid content and the brightness of the goldfish color on the value of a* (redness), b* (yellowness) and Huebut it does not affect the value of L* (lightness) and the growth of the goldfish

GROWTH AND EARLY REPRODUCTION DEVELOPMENT OF THE FIRST GENERATION OF SHEATFISH, Ompok miostoma (Vaillant, 1902) REARED IN CONTROLLED CONCRETE TANKS

This study was conducted to observe the growth and reproductive biology of wild sheatfish in controlled concrete tanks. The research was carried out from January to November 2020 at the Research Station for Freshwater Fisheries Germplasm, Cijeruk, Bogor. Sampled fish were captured from Cilala Lake in Bogor and Cipanas River in Sumedang, West Java. The collected fish were 14.6 ± 2.24 cm in length and 21.5 ± 9.19 g in weight. Growth and reproductive biology parameters were measured every 30 days. Cortisol, estradiol, vitellogenin, testosterone, glucose, and hemoglobin were also determined as the supporting parameters for gonadal maturity. Measured water quality parameters were temperature, pH, dissolved oxygen, ammonia, and alkalinity. The results showed that sheatfish grew with a specific growth rate of 0.4 ± 0.15%/day, FCR of 3.2 ± 0.26, and survival rate of 100%. Observation on the gonad maturity found that the fish studied were in the level-I and II. There are no significant differences regarding the supporting parameters (P>0.05) on the fish examined, despite the tendency of decreasing cortisol, and increasing testosterone. Based on the results, this study concludes that the observed fish can adapt, grow, and start to mature their gonads in their new environment, with temperature as one of the possible key factors influencing its gonad development.

EFFECT OF ADDITIONAL LEAF EXTRACT OF CIPLUKAN (Physalis angulata L) ON FEED ON THE GROWTH PERFORMANCE OF TILAPIA (Oreochromis niloticus)

The purpose of this study is to determine the effect of the effectiveness of the addition of ciplukan leaf extract (Physalis angulata L.) on feed to the growth performance of tilapia (Oreochromis niloticus). This research method uses a Complete RandomIzed Design (RAL). This study consisted of 4 treatments and 3 replays, so 12 experimental units were used. Dose treatment on P0 (feed without the addition of ciplukan leaf extract), P1 (Feed added ciplukan leaf extract with a dose of 4%), P2 (Feed added ciplukan leaf extract with a dose of 8%), and P3 (Feed added ciplukan leaf extract with a dose of 12%). This dosage treatment is based on Effendi &Hardi research (2014) with dose treatment of 4%, 8%, and 12% per 1kg of feed. the addition of ciplukan leaf extract with a concentration of 8% in feed provides absolute weight growth of (37.39±20.33), survival rate of (83.33±0.9 8), daily growth rate (1.25±0.60), and feed utilization efficiency of (4.67±0.58) and tend to be higher than other treatments.

Shugan Hewei Decoction Alleviates Cecum Mucosal Injury and Improves Depressive- and Anxiety-Like Behaviors in Chronic Stress Model Rats by Regulating Cecal Microbiota and Inhibiting NLRP3 Inflammasome

Chronic stress is a significant cause of depression, anxiety, and intestinal mucosal injury. Gut microbiota disturbances are also associated with these disorders. Shugan Hewei Decoction (SHD), which is a traditional Chinese medicine formula developed by our team, has shown superior therapeutic effects in the treatment of depression, anxiety, and functional gastrointestinal diseases caused by chronic stress. In this study, we investigated the modulatory effect of SHD on the cecal microbiota and cecum mucosal NOD-like receptor protein 3 (NLRP3) inflammasome in a chronic unpredictable stress (CUS)/social isolation rat model. After the SHD intervention, the CUS model rats showed improvements in their depressive- and anxiety-like behaviors, as well as sustained body weight growth and improved fecal characteristics. SHD improved the cecal microbiota diversity and changed the abundance of six microbial genera. A Spearman’s correlation analysis showed a strong correlation between the NLRP3 inflammasome and CUS-perturbed cecal biomarker microbiota. SHD regulated the excessive expression of NLRP3, ASC, caspase-1, interleukin-1β (IL-1β), and IL-18 in the serum and cecum mucosa induced by CUS, as well as the activation of the Toll-like receptor 4/nuclear factor-κB signaling cascades. Our results reveal the pharmacological mechanisms of SHD and provide a validated therapeutic method for the treatment of depression, anxiety, and cecum mucosal injury.

The effect of chitosan levels in feed on the growth and daily feed consumption of giant gourami (Osphronemus goramy)

Chitosan is an ingredient that can be used as a feed additive. In aquaculture, chitosan can be used as an antimicrobial, antifungal, antioxidant growth-promoting and immunostimulant. This study aims to determine the optimum addition of chitosan to feed to increase growth and daily feed consumption of giant gourami. The method used in this research is an experimental method using a completely randomized design (CRD), which consists of five treatments and three replications. The treatments used were (A) without chitosan (control), (B) 2.5 g/kg feed, (C) 5 g/kg feed, (D) 7.5 g/kg feed, and (E) giving chitosan of 10 g/kg feed. The test fish used were giant gourami with a 6 – 8 cm length and a weight of 8 – 10 g. The container used was hapa measuring 1 x 1 x 1 m3 with a density of 15 fish per treatment and 42 days of maintenance. The feeding rate was 3% of the fish biomass. Data from observations of length and weight were analyzed using Analysis of Variance (ANOVA) with a 95% confidence level; if there was a significant difference, Duncan's multiple distance test was performed. The results showed that addition of chitosan 7.5 g/kg feed was effective in improving growth on giant gourami. During maintenance, absolute length growth was 2.90 ± 0.10 cm, absolute weight growth was 15.33 ± 1.19 g, daily growth was 36.51 ± 2.84%, feed conversion ratio was 1.69 ± 0.06, and survival rate was 100 ± 0.00%. This research concluded that the optimum level of addition of chitosan to increase growth and daily feed consumption of giant gourami was 7.5 g/kg feed.Keywords:ChitosanFeed additiveGiant gouramiGrowth

Effects of “Bacuri” Seed Butter (Platonia insignis Mart.) on Metabolic Parameters in Hamsters with Diet-Induced Hypercholesterolemia

This study aimed to evaluate the effects of the treatment with bacuri seed butter (BB) on body weight, growth, body mass index, lipid profile, atherosclerotic indices, and liver function in dyslipidemic hamsters. Freshly weaned, male hamsters were divided into four groups: (1) normal group (NG)—maintained with standard chow (AIN-93G); (2) dyslipidemia group (DG)—maintained with hyperlipidemic chow (AIN-93G modified) throughout the follow-up period; (3) bacuri seed butter 25 mg/kg/day (BB-25); and (4) bacuri seed butter 50 mg/kg/day (BB-50). BB groups (25 and 50 mg/kg/day) were also maintained with hyperlipidemic chow throughout the follow-up period, and the treatment started after 21 days receiving a hyperlipidemic diet to induce hypercholesterolemia and maintained for 28 days. No significant differences in triglycerides and total cholesterol were observed for BB-25 and BB-50 groups when compared with NG and DG groups. On the contrary, BB-25 and BB-50 induced both increase of HDL-c (51.40 ± 1.69 and 51.00 ± 2.34, respectively) and decrease of LDL-c (103.80 ± 6.87 and 100.50 ± 3.95, respectively) when compared with DG (41.00 ± 2.94 and 132.70 ± 9.41, respectively). In addition, BB promoted a reduction in the risk of atherosclerotic disease by decreasing p < 0.05 the atherogenic index, coronary artery risk index, and LDL/CT ratio p < 0.05 and increasing HDL/CT ratio. On the contrary, no changes were observed in total cholesterol and triglyceride levels or in body weight, growth, body mass index, or liver function parameters. Thus, bacuri seed butter at doses of 25 and 50 mg/kg/day has positive repercussions on the lipid profile, more precisely on plasma HDL-c and LDL-c, and additionally promotes reduction in the risk of atherosclerosis in hamsters.

Pengaruh pemberian minuman campuran daun katuk, daun pepaya, dan kacang hijau terhadap produksi ASI dan berat badan bayi mencit

Foodstuffs rich in lactogogum are very useful in increasing milk production, especially in postpartum mothers. This study studied the effect of a mixed drink of katuk leaves, papaya leaves, and green beans on increasing breast milk production and weight growth of baby mice. This type of research is an experimental RAL with four treatments and three repetitions on female mice of the DDY strain aged 2,5–3 months, a total of 32 mice with six mice each. Mice were divided into four groups, two treatment groups and two control groups. Its treatment for 12 days postpartum. Data analysis used ANOVA and continued with the Duncan test at a 5% confidence interval. The study results found that there was a significant difference in the average milk production of the mother mice in the four groups (p= 0,003); there was no difference in the average total weight gain of mice during the four groups (p= 0,187). In conclusion, giving a functional drink a mixture of katuk leaves, papaya leaves, and green beans has the same potential as commercial katuk leaf extract but has not increased breast milk production in mice. Suggestion, further research is needed to measure prolactin hormone levels and milk quality

CẢI TIẾN VIỆC ĐỰC HÓA CÁ XIÊM (Betta splendens Regan, 1910) BẰNG CÁCH NGÂM TRONG 17α-METHYLTESTOSTERONE

Cá xiêm (Betta splendens) 1 ngày tuổi được đực hóa bằng cách ngâm trong túi polyethylene chứa 17α-methyltestosterone (17α-MT) ở các nồng độ 2,5; 5,0 và 7,5 mg/L có bơm oxy (thể tích oxy: thể tích nước bằng 2:1), mật độ 150 con/L trong 4 giờ. Kết quả cho thấy, tỷ lệ đực và đực hóa tăng khi tăng nồng độ hormone, với nồng độ 2,5 mg 17α-MT/L cá đạt tỷ lệ đực, tỷ lệ đực hóa là 88,89% và 78,26%. Nồng độ 5,0 mg 17α-MT/L và nồng độ 7,5 mg 17α-MT/L đạt tỷ lệ đực, tỷ lệ đực hóa là 100%. Khác biệt không có ý nghĩa (p>0,05) về tăng trưởng chiều dài và khối lượng của cá xiêm. Tỷ lệ sống 60 ngày sau khi nở là 54 - 89,89%, tỷ lệ sống giảm khi tăng nồng độ hormone 17α-MT trong nước ngâm. Kết quả cho thấy, nồng độ 2,5 mg 17α-MT/L là liều tối ưu để áp dụng chuyển đổi cá xiêm đực. Nồng độ 2,5 mg 17α-MT/L cá đạt tỷ lệ sống, hiệu suất đực hóa cao nhất là (86,22%, 76,81%). Với nhiều ưu điểm như đơn giản, chi phí thấp và có thể áp dụng ở mọi nơi nên có thể sản xuất cá xiêm đực bằng cách áp dụng phương pháp ngâm cá trong hormone 17α-MT với nồng độ 2,5 mg/L để đạt hiệu quả kinh tế tối ưu cho người nuôi. ABSTRACT The siamese fighting fish (Betta splendens) at one day old were masculinized by immersion in a polyethylene bag containing 17α-methyltestosterone (17α-MT) at concentrations of 2,5; 5,0 and 7,5 mg/L with oxygen pump (volume oxygen: water volume is equal to 2:1), density is 150 fish/L in 4 hours. The results showed that the male and masculinization ratios increased when the hormone concentration was increased, with a concentration of 2,5 mg 17α-MT/L, the male rate and masculinisation rate was 88,89%, and 78,26% respectively. Concentration of 5,0 mg 17α-M/L and concentration of 7,5 mg 17α-MT/L reached the male rate, the masculinization rate was 100%. The difference was not significant (p>0,05) in terms of length and weight growth of siamese fighting fish. The survival rate of 60 days after hatching was 54 - 89,89%, the survival rate decreases when the concentration of 17α-MT hormone in immersion water increases. The results showed that, concentration of 2,5 mg 17α-M/L was the optimal dose for converting male siamese fighting fish. Concentration of 2,5 mg 17α-MT/L, fish achieved the highest survival rate, masculinization efficiency respectively rate (86,22%, 76,81%). With many advantages such as simplicity, low cost and applicability everywhere, it is possible to produce male siamese fighting fish by applying the method of immersion fish in the hormone 17α-MT with a concentration of 2,5 mg/L to achieve efficiency economic efficiency optimal for farmers.