Transcriptomic analysis of flower color variation in the ornamental crabapple (Malus spp.) half-sib family through Illumina and PacBio Sequel sequencing

2020 ◽  
Vol 149 ◽  
pp. 27-35 ◽  
Author(s):  
Bin Huang ◽  
Hao Rong ◽  
Youju Ye ◽  
Zhouxian Ni ◽  
Meng Xu ◽  
...  
Keyword(s):  
Flower Color ◽  
Transcriptomic Analysis ◽  
Color Variation

scholarly journals Mechanism Underlying Color Variation in Calla Lily Spathes Based on Transcriptomic Analysis

2021 ◽  
pp. 1-16
Author(s):  
Ying Fang ◽  
Ting Lei ◽  
Yanmei Wu ◽  
Xuehua Jin
Keyword(s):  
Quantitative Polymerase Chain Reaction ◽  
Expression Patterns ◽  
Flower Color ◽  
Transcriptomic Analysis ◽  
Color Variation ◽  
Anthocyanin Synthesis ◽  
Helix Loop Helix ◽  
Calla Lily ◽  
Unique Shape ◽  
Glutathione S Transferases

The calla lily (Zantedeschia hybrida) is a valued ornamental plant due to its unique shape and color variations. To determine the mechanisms responsible for color development in the calla lily spathe, we conducted a comparative transcriptomic analysis of the spathes of the black [Black Girl (B)], pink [Romantic (P)], and white [Ventura (W)] cultivars. The gene expression patterns in six spathe colors, including the preceding three colors as well as the amaranth [Promise (N)], red [Figo (F)], and yellow [Sun Club (Y)] cultivars were analyzed by real-time quantitative polymerase chain reaction (PCR). Transcriptomic analysis identified 25,165 differentially expressed genes. The transcription abundance and expression level of genes annotated as anthocyanidin reductase (ANR1, ANR2), basic-helix-loop-helix (bHLH1), and glutathione S-transferases (GST1) were significantly upregulated in B, and the expression of anthocyanidin synthase (ANS) was highest in B except for N. However, chalcone isomerase (CHI2) and dihydroflavonol 4-reductase (DFR1, DFR2) were expressed at significantly lower levels in P, W, and Y. Correlation analysis revealed that bHLH1 might act as a positive regulator of ANS expression, promoting anthocyanin synthesis. Moreover, GST1-encoded proteins may be related to the accumulation and transport of both anthocyanin and procyanidin in the calla lily spathe. It is speculated that the formation of the black spathe is related to the accumulation of anthocyanins and procyanidins. However, the low expression of CHI2, DFR1, and DFR2 may result in the inhibition of anthocyanin synthesis, which may lead to lightening of the spathe color. This preliminary study revealed the mechanism responsible for calla lily spathe color, identifying the key genes involved, thus providing effective gene resources and a theoretical basis for flower color molecular breeding.

Relationship Between the Composition of Anthocyanins and Flower Color Variation in Hardy Water Lily (Nymphaea spp.) Cultivars

2013 ◽  
Vol 47 (5) ◽  
pp. 437-453
Author(s):  
Zhu Manlan ◽  
Wang Liangsheng ◽  
Zhang Huijin ◽  
Xu Yanjun ◽  
Zheng Xuchen ◽  
...  
Keyword(s):  
Flower Color ◽  
Color Variation ◽  
Water Lily

scholarly journals Transcriptomic and chemical analyses to identify candidate genes involved in color variation of sainfoin flowers

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Yu Qiao ◽  
Qiming Cheng ◽  
Yutong Zhang ◽  
Wei Yan ◽  
Fengyan Yi ◽  
...  
Keyword(s):  
Regulatory Networks ◽  
Flower Color ◽  
Color Variation ◽  
Anthocyanin Synthesis ◽  
Flower Formation ◽  
Factors Affecting ◽  
Phenylpropanoid Biosynthesis ◽  
Molecular Information ◽  
Illumina Hiseq4000 ◽  
Insight Into

Abstract Background Sainfoin (Onobrychis viciifolia Scop) is not only a high-quality legume forage, but also a nectar-producing plant. Therefore, the flower color of sainfoin is an important agronomic trait, but the factors affecting its flower phenotype are still unclear. To gain insights into the regulatory networks associated with metabolic pathways of coloration compounds (flavonoids or anthocyanins) and identify the key genes, we conducted a comprehensive analysis of the phenotype, metabolome and transcriptome of WF and AF of sainfoin. Results Delphinidin, petunidin and malvidin derivatives were the main anthocyanin compounds in the AF of sainfoin. These substances were not detected in the WF of sainfoin. The transcriptomes of WF and AF in sainfoin at the S1 and S3 stages were obtained using the Illumina HiSeq4000 platform. Overall, 10,166 (4273 upregulated and 5893 downregulated) and 15,334 (8174 upregulated and 7160 downregulated) DEGs were identified in flowers at S1 and S3 stages, respectively (WF-VS-AF). KEGG pathway annotations showed that 6396 unigenes were annotated to 120 pathways and contained 866 DEGs at S1 stages, and 6396 unigenes were annotated to 131 pathways and included 1546 DEGs at the S3 stage. Nine DEGs belonging to the “flavonoid biosynthesis”and “phenylpropanoid biosynthesis” pathways involved in flower color formation were identified and verified by RT-qPCR analyses. Among these DEGs, 4CL3, FLS, ANS, CHS, DFR and CHI2 exhibited downregulated expression, and F3H exhibited upregulated expression in the WF compared to the AF, resulting in a decrease in anthocyanin synthesis and the formation of WF in sainfoin. Conclusions This study is the first to use transcriptome technology to study the mechanism of white flower formation in sainfoin. Our transcriptome data will be a great enrichment of the genetic information for sainfoin. In addition, the data presented herein will provide valuable molecular information for genetic breeding and provide insight into the future study of flower color polymorphisms in sainfoin.

Transcriptome-sequencing analyses reveal putative genes related to flower color variation in Chinese Rosa rugosa

2018 ◽  
Vol 40 (3) ◽  
Author(s):  
Lixia Sheng ◽  
Wei Xia ◽  
Shu Zang ◽  
Yuqian Zeng ◽  
Xiaoyu Yuan ◽  
...  
Keyword(s):  
Transcriptome Sequencing ◽  
Flower Color ◽  
Color Variation ◽  
Rosa Rugosa

scholarly journals Cloning and Functional Characterization of Dihydroflavonol 4-Reductase Gene Involved in Anthocyanidin Biosynthesis of Grape Hyacinth

2019 ◽  
Vol 20 (19) ◽  
pp. 4743 ◽  
Author(s):  
Hongli Liu ◽  
Qian Lou ◽  
Junren Ma ◽  
Beibei Su ◽  
Zhuangzhuang Gao ◽  
...  
Keyword(s):  
Functional Characterization ◽  
Flower Colour ◽  
Flower Color ◽  
Color Variation ◽  
Blue Flower ◽  
Blue Color ◽  
Color Development ◽  
High Preference ◽  
Grape Hyacinth

Grape hyacinth (Muscari spp.) is a popular ornamental plant with bulbous flowers noted for their rich blue color. Muscari species have been thought to accumulate delphinidin and cyanidin rather than pelargonidin-type anthocyanins because their dihydroflavonol 4-reductase (DFR) does not efficiently reduce dihydrokaempferol. In our study, we clone a novel DFR gene from blue flowers of Muscari. aucheri. Quantitative real-time PCR (qRT-PCR) and anthocyanin analysis showed that the expression pattern of MaDFR had strong correlations with the accumulation of delphinidin, relatively weak correlations with cyanidin, and no correations with pelargonidin. However, in vitro enzymatic analysis revealed that the MaDFR enzyme can reduce all the three types of dihydroflavonols (dihydrokaempferol, dihydroquercetin, and dihydromyricetin), although it most preferred dihydromyricetin as a substrate to produce leucodelphinidin, the precursor of blue-hued delphinidin. This indicated that there may be other functional genes responsible for the loss of red pelargonidin-based pigments in Muscari. To further verify the substrate-specific selection domains of MaDFR, an assay of amino acid substitutions was conducted. The activity of MaDFR was not affected whenever the N135 or E146 site was mutated. However, when both of them were mutated, the catalytic activity of MaDFR was lost completely. The results suggest that both the N135 and E146 sites are essential for the activity of MaDFR. Additionally, the heterologous expression of MaDFR in tobacco (Nicotiana tabacum) resulted in increasing anthocyanin accumulation, leading to a darker flower color, which suggested that MaDFR was involved in color development in flowers. In summary, MaDFR has a high preference for dihydromyricetin, and it could be a powerful candidate gene for genetic engineering for blue flower colour modification. Our results also make a valuable contribution to understanding the basis of color variation in the genus Muscari.

Thigmatropic Tales

2017 ◽  
Keyword(s):  
Evolutionary Biology ◽  
Flower Color ◽  
Morning Glory ◽  
Color Variation ◽  
Interdisciplinary Education ◽  
Feminist Analysis ◽  
Doctoral Work ◽  
Morning Glories

This chapter revisits the author's doctoral work on the maintenance of flower color variation in morning glories to explore how a feminist analysis can help explain the shape and scope of this research. It traces the idea of variation and the shifting understanding of its significance in the field of evolutionary biology and moreover posits that an interdisciplinary education would have fundamentally reshaped the author's work on the evolutionary biology of morning glory flower color variation. Thus, inspired by the touch-sensitive thigmatropic tendrils of morning glories, which allow the plants to scale large objects and burrow into narrow crevices, this chapter narrates tales of the morning glories through the curious and adventurous tendrils of naturecultural storytelling.

scholarly journals Comparative transcriptome analyses reveal genes related to pigmentation in the petals of a flower color variation cultivar of Rhododendron obtusum

2021 ◽  
Author(s):  
Xiaobo Sun ◽  
Lisi He ◽  
Zhenhao Guo ◽  
Jiale Su ◽  
Xiaoqing Liu ◽  
...  
Keyword(s):  
Flower Color ◽  
Development Stage ◽  
Color Variation ◽  
Strongly Correlated ◽  
Anthocyanin Pathway ◽  
Phenylpropanoid Biosynthesis ◽  
Genetic Mechanisms ◽  
Research Goal ◽  
Woody Ornamental Plant ◽  
R2r3 Myb

Abstract Rhododendron is an important woody ornamental plant and breeding varieties with different colors is vital research goal. In this study, a flower color variation cultivar ‘Yanzhi Mi’ (pink petals) and the wild-type (WT) cultivar ‘Dayuanyangjin’ (white petals with pink stripes) were used as research objects, the pigment and transcriptome of their petals during different flower development (stage S1, S2, S3, S4 and S5) were analyzed and compared. The results showed that the derivatives of cyanidin, peonidin and pelargonidin may be responsible for the pink of mutant petals and S2 stage (buds showing color at the top but with the scales still present) was the key stage of flower color formation of mutant. In total, 412,910 transcripts and 2,780 differentially expressed genes (DEGs) were identified in pairwise comparisons of WT and mutant petals. GO and KEGG enrichment analyses of the DEGs showed that the ‘DNA-binding transcription factor activity’, ‘Flavonoid biosynthesis’ and ‘Phenylpropanoid biosynthesis’ were more active in mutant petals. Early anthocyanin pathway candidate DEGs (CHS3-CHS6, CHI, F3Hs and F3’H) were strongly correlated and up-regulated expression in mutant petals than in WT petals at S2 stage. These genes may be the key structural genes for the pink coloration of mutant petals. In the petals of mutant, two R2R3-MYB unigene (TRINITY_DN59015_c3_g2 and TRINITY_DN49281_c1_g6) were identified as repressors involved in anthocyanin regulation and were significantly down-regulated at S2 stage. This study shed light on the biochemistry and genetic mechanisms underlying the flower coloration in two Rhododendron obtusum cultivars.

scholarly journals Morphological and Biochemical Studies of the Yellow and Purple–red Petal Pigmentation in Paeonia delavayi

HortScience ◽  
2018 ◽  
Vol 53 (8) ◽  
pp. 1102-1108
Author(s):  
Qianqian Shi ◽  
Long Li ◽  
Lin Zhou ◽  
Yan Wang
Keyword(s):  
Southwest China ◽  
Genetic Resource ◽  
Flower Color ◽  
Color Variation ◽  
Chemical Analyses ◽  
Anthocyanidin Synthase ◽  
Yellow Color ◽  
Biochemical Studies ◽  
Flavone Synthase ◽  
Paeonia Delavayi

Paeonia delavayi is a species endemic to Southwest China and an important genetic resource for flower color breeding of tree peonies. The mechanisms underlying the flower coloration of this plant have not been fully elucidated. In this article, the petals of yellow-colored individual (Pl) and purple–red-colored individual (Pd) of P. delavayi were studied. And anatomical observations revealed that a large amount of yellow protoplasts and a small amount of colorless protoplasts were located in the yellow-colored Pl petals, whereas a mixture of purple, red, and pink protoplasts were observed in the purple–red-colored Pd petals. The Pl cells were subrotund and flat, whereas the Pd cells were irregularly polygon-shaped and bulging. Chemical analyses were performed, and the results indicated that significant differences occurred between the cell sap pH of the Pl and Pd flowers and large differences occurred in the contents of Fe and Al between Pl and Pd. Cyanidin- and peonidin-based anthocyanins with flavones and flavonols as copigments determined the Pd flower color, whereas chalcone 2 ′G with apigenin 7-O-neohesperidoside and chrysoeriol 7-O-glucoside as copigments determined the yellow color of Pl. Correspondingly, the genes dihydroflavonol 4-reductase (DFR) and anthocyanidin synthase (ANS) were significantly highly expressed in Pd, whereas chalcone isomerase (CHI), flavanone 3-hydroxylase (F3H), flavone synthase (FNS), flavonol synthase (FLS), flavonoid 7-O-glycosyltransferase (7GT), and 2′4′6′4-tetrahydroxychalcone 2′-glucosyltransferase (THC) had high transcript levels in Pl relative to Pd. The results indicate that the color variation of P. delavayi petals may be related to a delicately controlled balance of the aforementioned factors.

Transcriptome analysis of flower color variation in five Rhododendron species (Ericaceae)

2021 ◽  
Vol 44 (3) ◽  
pp. 685-695
Author(s):  
Zhiliang Li ◽  
Qiaofeng Yang ◽  
Xue Dong ◽  
Yu Zhu ◽  
Shuang Zhao ◽  
...  
Keyword(s):  
Transcriptome Analysis ◽  
Flower Color ◽  
Color Variation
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