Investigations on effect of dry root (<i>F. solani</i> var. <i>coeruleum</i> and <i>F. sulphureum</i>) pathogens on three potato cultivares Azalia, Pola, Odra chemical composition were carried out. Content of total nitrogen and protein, total saccharides, reducting and bisaccharides and vit. C was investigated. Significant decrease of total and protein nitrogen content in potatoes affected by <i>Fusarium</i> in relation to intact tubers was found. Moreover increase of reducting saccharides and bisaccharides in potatoes infected by <i>Fusarium</i> compared with controls was observed. Level of vit. C was decreased in tubers affected by <i>Fusarium</i> in all potato cultivars. In this experiment changes in chemical composition content in potato tubers were connected with a kind of dry rot patogen.