Assessment of fertility potential index of some soils of Moheshkhali betel leaf (Piper betle L.) estate

Fifteen soil samples (0-15cm depth) and fifteen betel leaf samples from Bara Moheshkhali, Choto Moheshkhali, Hoanak, Kalmarchora, Shaplapur union of Moheshkhali upazila, Cox’s Bazar district were collected on December-January (2020-2021). It was collected for the purpose of evaluating the color, particle density, moisture content, texture, pH, EC and organic matter content of soils and total nitrogen, phosphorous, potassium and sulfur of the soils and total protein content, nitrogen, phosphorus, potassium and sulfur of leaves. Maximum sampled soil was containing yellowish red color when moist and showed brown color when dried. Some of them were flood plain soil and that’s why showed grey color during dry and moist condition. Mean particle density was 2.49 g/cm3 and mean moisture content was 13.95%. Average sand, silt and clay were 65.08%, 18.56% and 19.26%, respectively. The texture of the soils was sandy loam to clay loam. The sand was by far the dominant fraction in the soils. Mean pH value was 5.63, organic matter was 0.30% and EC was 99.38μS/cm. Mean total nitrogen, phosphorus, potassium and sulfur of the soils were 0.04, 0.46, 0.25 and 0.26%, respectively. The mean concentration of protein, nitrogen, phosphorus, potassium and sulfur in the betel leaf samples were 20.56, 3.29, 0.39, 0.79 and 0.20%, respectively. This investigation focuses on the fertility status and physico-chemical properties of soil in betel leaf garden of only hilly island of Bangladesh, Moheshkhali. J. Biodivers. Conserv. Bioresour. Manag. 2021, 7(1): 25-32

Effect of Bacillus amyloliquefaciens and Bacillus subtilis on fermentation, dynamics of bacterial community and their functional shifts of whole-plant corn silage

Background Bacillus amyloliquefaciens (BA) and Bacillus subtilis (BS) are usually used as feed supplements directly or bacterial inoculants in biological feeds for animals. However, few research have reported the effects of BA and BS on fermentation characteristics and bacterial community successions of whole-plant corn silage during ensiling. If the BA and BS inoculants have positive effects on silages, then they could not only improve fermentation characteristics, but also deliver BA or BS viable cells to ruminants, which would play its probiotic effect. Therefore, the objectives of this study were to investigate the effects of BA and BS on the fermentation, chemical characteristics, bacterial community and their metabolic pathway of whole-plant corn silage. Results Freshly chopped whole-plant corn was inoculated without or with BA and BS, respectively, and ensiled for 1, 3, 7, 14 and 60 d. Results showed that BA and BS inoculations increased lactic acid concentrations of whole-plant corn silages compared with control, and BA inoculation decreased acetic acid concentrations, whereas BS inoculation decreased fiber contents and increased crude protein (CP) content. Higher water-soluble carbohydrate contents and lower starch contents were observed in BA- and BS-inoculated silages compared with that in control. The decreased CP content and increased non-protein nitrogen content were observed in BA-inoculated silage, which was consistent with the higher amino acid metabolism abundances observed in BA-inoculated silage. In addition, it was noteworthy that BA and BS inoculations increased the metabolism of cofactors and vitamins, and decreased the relative abundances of drug resistance: antimicrobial pathways. We also found that the bacterial metabolism pathways were clearly separated into three clusters based on the ensiling times of whole-plant corn silage in the present study. There were no significant differences in bacterial community compositions among the three groups during ensiling. However, BA and BS inoculations decreased the relative abundances of undesirable bacteria such as Acetobacter and Acinetobacter. Conclusion Our findings suggested that the BS strain was more suitable as silage inoculants than the BA strain in whole-plant corn silage in this study.

Saccharomyces cerevisiae Dehydrated Culture Modulates Fecal Microbiota and Improves Innate Immunity of Adult Dogs

Saccharomyces cerevisiae yeast culture can be dehydrated, and it has a potential prebiotic effect. This study evaluated the effects of supplementing increasing levels of dehydrated yeast culture (DYC) of Saccharomyces cerevisiae (Original XPC™, Diamond V, Cedar Rapids, IA, USA) on fecal microbiota, nutrient digestibility, and fermentative and immunological parameters of healthy adult dogs. Eighteen adult male and female dogs with a mean body weight of 15.8 ± 7.37 kg were randomly assigned to three experimental treatments: CD (control diet), DYC 0.3 (control diet with 0.3% DYC) and DYC 0.6 (control diet with 0.6% DYC). After 21 days of acclimation, fecal samples were collected for analysis of nutrient digestibility, microbiota and fecal fermentation products. On the last day, the blood samples were collected for the analysis of immunological parameters. The microbiome profile was assessed by the Illumina sequencing method, which allowed identifying the population of each bacterial phylum and genus. The statistical analyses were performed using the SAS software and the Tukey test for multiple comparison (p < 0.05). Our results suggest that the addition of DYC increased the percentage of the phyla Actinobacteria and Firmicutes (p = 0.0048 and p < 0.0001, respectively) and reduced that of the phylum Fusobacteria (p = 0.0008). Regardless of the inclusion level, the yeast addition promoted reduction of the genera Allobaculum and Fusobacterium (p = 0.0265 and p = 0.0006, respectively) and increased (p = 0.0059) that of the genus Clostridium. At the highest prebiotic inclusion level (DYC 0.6), an increase (p = 0.0052) in the genus Collinsella and decrease (p = 0.0003) in Prevotella were observed. Besides that, the inclusion of the additive improved the apparent digestibility of the crude fiber and decreased the digestibility of crude protein, nitrogen-free extract and metabolizable energy (p < 0.05). There were no significant changes in the production of volatile organic compounds. However, an increase in propionate production was observed (p = 0.05). In addition, the inclusion of yeast resulted in an increased phagocytosis index in both treatments (p = 0.01). The addition of 0.3 and 0.6% DYC to the diet of dogs wase able to modulate the proportions of some phyla and genera in healthy dogs, in addition to yielding changes in nutrient digestibility, fermentative products and immunity in healthy adult dogs, indicating that this additive can modulate fecal microbiota and be included in dog nutrition.

DEVELOPMENT OF TECHNOLOGY FOR OBTAINING MULTIPLE EMULSION OF OVARIAN LIQUID (CAVIAR SOL), ENSURING THE DURATION OF ITS STORAGE

Дефицит пищевого белка обусловливает актуальность развития отечественного производства пищевых белковых продуктов из биоресурсов вторичного происхождения. Овариальная жидкость (икорный золь) – вторичный продукт икорного производства – может быть использована как физиологически функциональный ингредиент для пищевых продуктов сбалансированного питания. Применяемые способы сохранности овариальной жидкости (ОЖ) приводят к изменению нативных свойств ее компонентов за счет температурного или химического воздействия. Высказано предположение, что капсулирование во множественную эмульсию ОЖ позволит сохранить ее нативные свойства и увеличить срок хранения. Цель настоящего исследования – разработка технологии получения множественной эмульсии ОЖ и установление эффективности новой формы продукта при хранении. Объектом исследования была ОЖ, полученная при заборе икры осетровых рыб с применением метода прижизненного получения. Множественную эмульсию готовили двухступенчатым способом. На первом этапе получали стандартную эмульсию вода–масло, которую затем использовали в качестве одной из фаз для множественной эмульсии вода–масло–ОЖ. Полученный продукт и контрольный образец (ОЖ, прошедшая только фильтрацию) размещали в чашках Петри и хранили в термостатируемой холодильной камере при температуре не ниже 4°С в течение 40 сут. Через каждые 5 сут в отобранных пробах определяли по методу Кьельдаля содержание белковых веществ, небелкового азота и азота летучих оснований. Установлено, что при хранении в ОЖ, прошедшей только фильтрацию, белок разрушался быстрее и его содержание через 40 сут снизилось на 34%, тогда как в ОЖ, заключенной во множественную эмульсию, этот показатель снизился только на 5,9%, что коррелирует со скоростью накопления продуктов распада белков в ОЖ после 40 сут хранения. Предложенный способ получения множественной эмульсии ОЖ позволяет обеспечить сохранность ее белкового комплекса в процессе хранения и разработать новый ассортимент физиологически функциональных продуктов с использованием отечественного биологически ценного пищевого ингредиента. The shortage of dietary protein determines the relevance of the development of domestic production of food protein products from bioresources of secondary origin. Ovarian liquid (caviar ash), or caviar sol, – a secondary product of caviar production – can be used as a physiologically functional ingredient for balanced nutrition foods. The applied methods of preservation of ovarian fluid (OF) lead to a change in the native properties of its components due to temperature or chemical exposure. It is suggested that encapsulation in a multiple emulsion of OF will preserve its native properties and increase the shelf life. The purpose of this study is to develop a technology for obtaining multiple OF emulsion and to establish the effectiveness of a new product form during storage. The object of the study was the OF obtained by sampling the caviar of sturgeon fish using the method of lifetime production. The multiple emulsion was prepared in a two-stage way. At the first stage a standard water–oil emulsion was obtained, which was then used as one of the phases for a multiple water–oil–OF emulsion. The resulting product and the control sample (OF that passed only filtration) were placed in Petri dishes and stored in a thermostatically controlled refrigerator at a temperature of at least 4°C for 40 days. Every 5 days the content of protein substances, non-protein nitrogen and nitrogen of volatile bases was determined in the selected samples by the Kjeldahl method. It was found that when stored in the OF that passed only filtration, the protein was destroyed faster and its content decreased by 34% after 40 days, whereas in the OF enclosed in a multiple emulsion, this indicator decreased by only 5.9%, which correlates with the rate of accumulation of protein breakdown products in the OF after 40 days of storage. The proposed method for obtaining a multiple emulsion of COOLANT makes it possible to ensure the safety of its protein complex during storage and to develop a new range of physiologically functional products using a domestic biologically valuable food ingredient.

Compositional Features and Nutritional Value of Pig Brain: Potential and Challenges as a Sustainable Source of Nutrients

The goal of this study was to establish the nutritional value and compositional properties of the brains of crossbred pigs (Landrace–Large white–Duroc (LLD)), in order to realize the zero-waste concept and increase the use of by-products in the sustainable meat industry. Fat (9.25% fresh weight (fw)) and protein (7.25% fw) were the principal dry matters of pig brain, followed by carbohydrate and ash. Phospholipid and cholesterol had a 3:1 ratio. Pig brain had a red tone (L* = 63.88, a* = 5.60, and b* = 15.43) and a high iron content (66 mg/kg) due to a total heme protein concentration of 1.31 g/100 g fw. The most prevalent macro-element was phosphorus (14 g/kg), followed by potassium, sodium, calcium, and magnesium. Zinc, copper, and manganese were among the other trace elements discovered. The most prevalent nitrogenous constituents were alkali-soluble protein, followed by water-soluble protein, stromal protein, salt-soluble protein, and non-protein nitrogen. Essential amino acids were abundant in pig brain (44% of total amino acids), particularly leucine (28.57 mg/g protein), threonine, valine, and lysine. The total lipid, neutral, and polar lipid fractions of the pig brain had different fatty acid compositions. The largest amount was observed in saturated fatty acids (SFA), followed by monounsaturated fatty acids (MUFA) and polyunsaturated fatty acids (PUFA). Stearic acid and palmitic acid were the most common SFA. Oleic acid was the most prevalent MUFA, while docosahexaenoic acid was the most common PUFA. Thus, the pig brain can be used in food formulations as a source of nutrients.

Microbial Characterization of Yellow Curing Process of Codfish

Yellow cured codfish has a typical yellow colour, distinctive taste, and low salt content due to its special curing process of the raw salted codfish involving several soaks in water of the raw salted codfish, alternated with drying steps. The purpose of this study was to assess the main functional groups of bacteria involved in this process and relate them with physicochemical properties of the product. A total of 28 codfish from Iceland were supplied by two local companies. Seven stages of the curing process were analyzed. From each of these seven stages, four fish samples were collected to carry out the microbial and physicochemical analyses (moisture, salt content, pH, total volatile basic nitrogen (TVB-N), and trimethylamine nitrogen (TMA-N)). Bacteria counts were performed using the MPN method and adequate culture media for aerobic, proteolytic, sulphite-reducing, biogenic amine, and trimethylamine-producing and ammonifying bacteria. Strains isolated from the highest dilutions with microbial growth were used to characterize the predominant bacteria. The results showed that total aerobic counts increased from 3.9 log MPN/g in raw salted codfish to 5.9 log MPN/g in the final. Proteolytic, ammonifying, and trimethylamine bacteria producers also increased to 8, 7.5, and 6.5 log MPN/g, respectively. The salt content decreases (from 17% until 8%) and moisture increases (53% until 67%) during the salted-raw-codfish soaking, favoring sulphite-reducing and biogenic amine-producing species, confirming that desalting enhances potential spoilers. The subsequent drying step benefits proteolytic, ammonifying, and trimethylamine-producing bacteria, with a corresponding non-protein-nitrogen content (TVB-N and TMA-N) increase. The dominant bacteria during yellow curing belong to the genera Staphylococcus, Psychrobacter, Pseudomonas, and Alcaligenes with a clear positive correlation between the content of Staphylococcus and Psychrobacter and TVB-N and TMA-N concentration. Staphylococcus spp. are the dominant bacteria in the steps where the product has a higher salt concentration; thus, it could be particularly useful as an indicator to control the industrially yellow curing process and could have an important role in the development of the final characteristics of this product.

Chemical Changes The Spent Hen Meat After A Tenderization Process Solution of Sodium Chloride

In this study, the old chicken meat found in the market after slaughtering and cleaning was carried out by the saline application, which was as follows T: the control treatment. T2, T3 and T4 were tenerized spent hen meat with sodium chloride by 10, 20 and 30 g/L water for 12 hours respectively. Results show that the collagen was a significant increase (P≤0.05) in T2, T3, T4 compared with the control of white muscle control and the total concentration of collagen was a significant decrease (P≤0.05) in saline annulus coefficients compared to control of dark muscle. The total concentration of soluble nitrogen was a significant decrease (P≤0.05) in saline-induced salinity coefficients compared with white muscle control. It is also noted that the concentration of protein nitrogen in the white muscle has decreased significantly (P≤0.05) in the coefficients of the antigen compared to the control treatment.

Comparison between conventional urea and slow-release urea on concentration of ammonia in the rumen: A meta-analysis

Urea is a source of Non-Protein Nitrogen (NPN). The utilization of urea in the ration is useful for increasing digestibility, dry matter intake, and increasing protein content. This study aims to compare the utilization of conventional urea and Slow Release Urea (SRU) to reduce ammonia concentration by in vivo study using meta-analysis. A total of 13 studies were obtained that consisted of 41 data points. The parameters in this study include pH, Dry Matter Intake (DMI), Volatile Fatty Acid (VFA), ammonia concentration, and nitrogen intake. The database compiled was statistically analyzed using a mixed model method. Different studies were considered as random effects, and the level of urea was treated as fixed effects. The model statistics used were the p-values and the Akaike information criterion. The significance of an effect was stated when its p-value was <0.05. The results revealed that level SRU and conventional urea had a significant linear effect on ammonia concentration, DMI, VFA, pH and nitrogen intake. However, the effect of giving SRU was better than conventional urea. It can be concluded that SRU can control ammonia concentration, DMI, VFA, pH and nitrogen intake better than urea.

Use of Rapid Capillary Zone Electrophoresis to Determine Amino Acids Indicators of Herring Ripening during Salting

Currently, herring fillets are salted with acetic acid to activate muscle proteases. This causes a change in the composition of free amino acids, compared to salting of whole fish with viscera proteases. Therefore, old indicators of the ripening dynamics of salted fish based on amino acids are not current. Determination of free amino acids can be performed by many methods, but most are labor intensive and expensive. Therefore, a capillary electrophoresis method without derivatization (CZE) was used to determine the actual ripening rates of salted herring fillets. A group of hydrophobic and basic amino acids were determined in trichloroacetic acid (TCA) extracts of meat and brine to develop 16 indicators. Statistical regression analysis of the indicators (R2adj, RMSE, cluster analysis) followed by principal component analysis (PCA) correlation analysis of the indicators vs sensory evaluation parameters of texture and TPA-hardness of salted fillet meat allowed the choice of the most precise indicators. The best indicator in meat was Phe/Tyr-height, which value increased during salting. A more precise indicator of ripening was His/Tyr-height in brine, which value decreased during salting. Sensory evaluation parameters of salted herring texture correlated strongly with TPA-hardness and traditional indicators such as non-protein nitrogen and protein hydrolysis product fraction content. However, the most precise indicators were those obtained from amino acids determined by the CZE method. Results obtained in this study may be suitable for fast monitoring of the salted herring ripening process in industry.