The association between bulk tank milk analysis for raw milk quality and on-farm management practices

2010 ◽  
Vol 95 (1-2) ◽  
pp. 32-40 ◽  
Author(s):  
A.M. Elmoslemany ◽  
G.P. Keefe ◽  
I.R. Dohoo ◽  
J.J. Wichtel ◽  
H. Stryhn ◽  
...  
2018 ◽  
Vol 50 (7) ◽  
pp. 1605-1610
Author(s):  
Cristina Simões Cortinhas ◽  
Bruno Garcia Botaro ◽  
Susana Nori de Macedo ◽  
Marcos Veiga dos Santos

2009 ◽  
Vol 76 (4) ◽  
pp. 483-489 ◽  
Author(s):  
Maria Åkerstedt ◽  
Karin Persson Waller ◽  
Åse Sternesjö

The aim of the present study was to evaluate relationships between the presence of the two major bovine acute phase proteins haptoglobin (Hp) and serum amyloid A (SAA) and raw milk quality parameters in bulk tank milk samples. Hp and SAA have been suggested as specific markers of mastitis but recently also as markers for raw milk quality. Since mastitis has detrimental effects on milk quality, it is important to investigate whether the presence of Hp or SAA indicates such changes in the composition and properties of the milk. Bulk tank milk samples (n=91) were analysed for Hp, SAA, total protein, casein, whey protein, proteolysis, fat, lactose, somatic cell count and coagulating properties. Samples with detectable levels of Hp had lower casein content, casein number and lactose content, but higher proteolysis than samples without Hp. Samples with detectable levels of SAA had lower casein number and lactose content, but higher whey protein content than samples without SAA. The presence of acute phase proteins in bulk tank milk is suggested as an indicator for unfavourable changes in the milk composition, e.g. protein quality, due to udder health disturbances, with economical implications for the dairy industry.


2014 ◽  
Vol 97 (7) ◽  
pp. 4083-4096 ◽  
Author(s):  
S.N. Masiello ◽  
N.H. Martin ◽  
R.D. Watters ◽  
D.M. Galton ◽  
Y.H. Schukken ◽  
...  

2009 ◽  
Vol 76 (2) ◽  
pp. 195-201 ◽  
Author(s):  
Erik Wickström ◽  
Karin Persson-Waller ◽  
Helena Lindmark-Månsson ◽  
Karin Östensson ◽  
Åse Sternesjö

The somatic cell count (SCC) in bovine bulk tank milk is presently used as an indicator of raw milk quality, reflecting the udder health status of the herd. During mastitis, SCC increases, mostly owing to an influx of polymorphonuclear leucocytes (PMN) from blood into milk, with a concomitant change in milk composition. Bulk tank milk samples were categorized according to their SCC, as well as polymorphonuclear leucocyte count (PMNC), to study relationships between SCC, PMNC and various raw milk quality traits, i.e. contents of total protein, whey protein, casein, fat and lactose, casein number, proteolysis and rheological properties. The proportion of PMN, obtained by direct microscopy, was significantly higher in samples with high SCC compared with low SCC samples. SCC and PMNC were strongly correlated, yielding a correlation coefficient of 0·85. High SCC samples had lower lactose and casein contents, lower casein number and more proteolysis than low SCC samples. Samples with high PMNC had a lower casein number than low PMNC samples. Samples with high and low SCC or PMNC did not differ in respect to rheological properties. Our results do not indicate that PMNC is a better biomarker than SCC for raw bulk tank milk quality, as previously proposed.


2019 ◽  
Vol 123 ◽  
pp. 77-83 ◽  
Author(s):  
Francesca Dell'Orco ◽  
Claudia Gusmara ◽  
Monica Loiacono ◽  
Theo Gugliotta ◽  
Francesca Albonico ◽  
...  

2003 ◽  
Vol 66 (12) ◽  
pp. 2349-2354 ◽  
Author(s):  
MICHAEL L. PERDUE ◽  
JEFF KARNS ◽  
JIM HIGGINS ◽  
JO ANN VAN KESSEL

A preparation of Bacillus anthracis (Sterne strain) spores was used to evaluate commercially available reagents and portable equipment for detecting anthrax contamination by using real-time PCR and was used to assess the fate of spores added directly to bulk tank milk. The Ruggedized Advanced Pathogen Identification Device (RAPID) was employed to detect spores in raw milk down to a concentration of 2,500 spores per ml. Commercially available primers and probes developed to detect either the protective antigen gene or the lethal factor gene both provided easily read positive signals with the RAPID following extraction from milk with a commercially available DNA extraction kit. Nucleotide sequence analysis of the vrrA gene with the use of DNA extracted from spiked milk provided molecular data that readily identified the spores as B. anthracis with a 100% BLAST match to the Sterne and Ames strains and easily distinguished them from B. cereus. Physical-fate and thermal-stability studies demonstrated that spores and vegetative cells have a strong affinity for the cream fraction of whole milk. A single treatment at standard pasteurization temperatures, while 100% lethal to vegetative cells, had no effect on spore viability even 14 days after the treatment. Twenty-four hours after the first treatment, a second treatment at 72°C for 15 s reduced the viability of the population by ca. 99% but still did not kill all of the spores. From these studies, we conclude that standard pasteurization techniques for milk would have little effect on the viability of B. anthracis spores and that raw or pasteurized milk poses no obstacles to the rapid detection of the spores by molecular techniques.


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