Effect of carryover and presampling procedures on the results of real-time PCR used for diagnosis of bovine intramammary infections with Streptococcus agalactiae at routine milk recordings

2014 ◽  
Vol 113 (4) ◽  
pp. 512-521 ◽  
Author(s):  
Yasser S. Mahmmod ◽  
Marshal M. Mweu ◽  
Søren S. Nielsen ◽  
Jørgen Katholm ◽  
Ilka C. Klaas
Keyword(s):  
Real Time ◽  
Streptococcus Agalactiae ◽  
Real Time Pcr ◽  
Intramammary Infections

Direct identification of Streptococcus agalactiae and capsular type by real-time PCR in vaginal swabs from pregnant women

2015 ◽  
Vol 21 (1) ◽  
pp. 34-38 ◽  
Author(s):  
Miyuki Morozumi ◽  
Naoko Chiba ◽  
Yuko Igarashi ◽  
Naoki Mitsuhashi ◽  
Takeaki Wajima ◽  
...  
Keyword(s):  
Pregnant Women ◽  
Real Time ◽  
Streptococcus Agalactiae ◽  
Real Time Pcr ◽  
Capsular Type ◽  
Direct Identification ◽  
Vaginal Swabs

scholarly journals Using Real-Time PCR to Investigate Some of Antibiotic Resistance Genes from Streptococcus agalactiae Isolates from ewe Mastitis cases in Nineveh province

2020 ◽  
Vol 17 (3(Suppl.)) ◽  
pp. 0931
Author(s):  
Ayman Mohamed Jaber Albanna ◽  
Aseel A. H. Al-Layla
Keyword(s):  
Antibiotic Resistance ◽  
Real Time ◽  
Resistance Genes ◽  
Streptococcus Agalactiae ◽  
Real Time Pcr ◽  
Bacterial Resistance ◽  
Single Gene ◽  
Antibiotic Resistance Genes ◽  
Antibiotics Resistance ◽  
Dilution Method

In this study, from a total of 856 mastitis cases in lactating ewes, only 34 Streptococcus agalactiae isolates showed various types of resistance to three types of antibiotics (Penicillin, Erythromycin and Tetracycline). St. agalactiae isolates were identified according to the standard methods, including a new suggested technique called specific Chromogenic agar. It was found that antibiotic bacterial resistance was clearly identified by using MIC-microplate assay (dilution method). Also, by real-time PCR technique, it was determined that there were three antibiotics genes resistance ( pbp2b, tetO and mefA ). The high percentage of isolate carried of a single gene which was the Tetracycline (20.59%) followed by percentage Penicillin was (17.65%) and the lowest was in Erythromycin (11.77%). However, there were many isolates that carried two genes of antibiotics resistance represented by Penicillin and Erythromycin with collective present of 38.22%, and for the Penicillin and Tetracycline, the percentage was found to be 11.77%. In contrast, no common gene with two antibiotics (Erythromycin and  Tetracycline) was detected. On the other hand, it was found that no bacterial sharing with three kinds of antibiotic resistance genes ( pbp2b, tetO and mefA ). This study has revealed that the St. agalactiae isolates did induce recurrent mastitis in lactating Iraqi's ewes. 

scholarly journals Development of a melting-curve based multiplex real-time PCR assay for simultaneous detection of Streptococcus agalactiae and genes encoding resistance to macrolides and lincosamides

2018 ◽  
Vol 18 (1) ◽  
Author(s):  
Eliane Saori Otaguiri ◽  
Ana Elisa Belotto Morguette ◽  
Alexandre Tadachi Morey ◽  
Eliandro Reis Tavares ◽  
Gilselena Kerbauy ◽  
...  
Keyword(s):  
Real Time ◽  
Streptococcus Agalactiae ◽  
Real Time Pcr ◽  
Simultaneous Detection ◽  
Melting Curve ◽  
Pcr Assay ◽  
Genes Encoding

Non-culture detection of Streptococcus agalactiae (Lancefield group B Streptococcus) in clinical samples by real-time PCR

2012 ◽  
Vol 61 (8) ◽  
pp. 1086-1090 ◽  
Author(s):  
Aruni de Zoysa ◽  
Kirstin Edwards ◽  
Saheer Gharbia ◽  
Anthony Underwood ◽  
André Charlett ◽  
...  
Keyword(s):  
Real Time ◽  
Streptococcus Agalactiae ◽  
Real Time Pcr ◽  
Group B Streptococcus ◽  
Clinical Samples ◽  
Group B ◽  
Lancefield Group
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