Profile of hydrolases and biogas production during two-stage mesophilic anaerobic digestion of solid potato waste

2005 ◽  
Vol 40 (9) ◽  
pp. 2945-2952 ◽  
Author(s):  
W. Parawira ◽  
M. Murto ◽  
J.S. Read ◽  
B. Mattiasson
2018 ◽  
Vol 7 (3.36) ◽  
pp. 170
Author(s):  
Umar M. Ibrahim ◽  
Saeed I. Ahmed ◽  
Babagana Gutti ◽  
Idris M. Muhammad ◽  
Usman D. Hamza ◽  
...  

The combination of Irish potato waste (IPW) and poultry waste (PW) can form a synergy resulting into an effective substrate for a better biogas production due to some materials they contain. In this work, optimization and kinetic study of biogas production from anaerobic digestion of IPW and PW was investigated. Response surface methodology (RSM) was applied to optimize conditions such as initial pH, solids concentrations and waste ratios. The anaerobic digestion of the two wastes was carried out in the mesophilic condition and Box-Behnken design (BBD) was used to develop and analyze a predictive model which describes the biogas yield. The results revealed that there is a good fit between the experimental and the predicted biogas yield as revealed by the coefficient of determination (R2) value of 97.93%. Optimization using quadratic RSM predicts biogas yield of 19.75% at the optimal conditions of initial pH value 7.28, solids concentration (w/v) 9.85% and waste ratio (IPW:PW) 45:55%. The reaction was observed to have followed a first order kinetics having R2 and relative squared error (RSE) values of 90.61 and 9.63% respectively. Kinetic parameters, such as rate constant and half-life of the biogas yield were evaluated at optimum conditions to be 0.0392 day-1 and 17.68 days respectively. The optimum conditions and kinetic parameters generated from this research can be used to design real bio-digesters, monitor substrate concentrations, simulate biochemical processes and predict performance of bio-digesters using IPW and PW as substrate.  


2021 ◽  
Author(s):  
Napisa Pattharaprachayakul ◽  
Jintana Jitpupakdee ◽  
Worakrit Suvajittanont ◽  
Dudsadee Uttapap ◽  
Vilai Rungsardthong

2017 ◽  
Vol 1 (1) ◽  
pp. 1 ◽  
Author(s):  
Purwinda Iriani ◽  
Yanti Suprianti ◽  
Fitria Yulistiani

Produksi biogas pada skala rumah tangga umumnya menggunakan teknologi fermentasi anaerobik di dalam satu biodigester (satu tahap), yang mengakomodasi dua tahap utama prinsip pembentukan biogas, yakni tahap asetogenesis dan tahap metanogenesis. Permasalahan yang muncul dari penggunaan digester biogas satu tahap adalah ketidakseimbangan proses fermentasi (peningkatan laju beban organik, waktu retensi senyawa organik yang lebih cepat, dan produktivitas biogas yang menjadi tidak maksimal). Untuk mengatasi hal tersebut, dilakukan penelitian yang bertujuan melakukan produksi biogas melalui sistem fermentasi anaerobik dua-tahap (two-stage anaerobic digestion), yang didukung dengan pengaturan pH pada proses metanogenik. Pada penelitian ini telah dilakukan proses aklimatisasi (aktivasi) bakteri yang menunjang proses asetogenik dan metanogenik pada skala laboratorium (19 L), dan selanjutnya menjadi inokulum untuk proses fermentasi skala pilot dengan kapasitas biodigester asetogenik 125 L dan metanogenik 500 L. Hasil proses aklimatisasi bakteri asetogenik pada media kotoran sapi menunjukkan adanya kestabilan pH yang dibutuhkan untuk reaksi asetogenik, yaitu pada kisaran pH 5-6, sedangkan kontrol menunjukkan perubahan pH yang masih ada di rentang pH netral yaitu 6-7. Kotoran sapi yang telah melalui proses asetogenik selama 2 minggu (pH awal 5,5), menjadi bahan baku pembuatan biogas pada digester metanogenik. Hasil dari proses metanogenik menunjukkan terjadinya peningkatan volume biogas dan komposisi gas metana (CH4) di dalam biogas. Komposisi CH4 tertinggi diperoleh pada hari ke-20 yakni 74,82% dengan volume produksi biogas tertinggi ada pada hari ke-22, dengan laju 8,87 L/hari. Potensi energi tertinggi yang diperoleh mencapai 217,66 kJ/hari.Generally, biogas production on the household scale is using one-stage anaerobic fermentation technology, which accommodates two main processes of biogas production, namely acetogenesis and methanogenesis. An obstacle of using one-stage biogas digester is the imbalance of the fermentation process that indicated by the increase of organic load rate and shorter retention time that lead to un-optimal biogas productivity. This research undertook the application of two-stage anaerobic digestion, supported by adjusting the initial pH for both acetogenic and methanogenic processes. Firstly, the research initiated by acclimatization (activation) process of acetogenic and methanogenic bacteria via fermentation in laboratory scale (19 L) digesters, separately. The results of acetogenic bacteria acclimatization process on cow dung media showed the pH stability needed for the reaction acetogenic, in the range of 5-6, while the control showed the pH changes still in the neutral pH range (6-7). The substrate from lab-scale acetogenic and methanogenic digester, then used as a starter for pilot-scale digester (125 L and 500 L, respectively). The mixture of water and cow dung were adjusted at initial pH 5.5 on acetogenic digester for 2 weeks. Those material were used for biogas production in the methanogenic digester. The result of the methanogenic process showed an increasing volume of biogas and the composition of methane (CH4) in the biogas. The highest CH4 composition was obtained on the 20th day, which reached 74.82%, and the highest volume of biogas production was at day 22, with the rate of 8.87 L/day. The highest energy potential obtained was 217.66 kJ/day.


PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e9693
Author(s):  
Wattananarong Markphan ◽  
Chonticha Mamimin ◽  
Wantanasak Suksong ◽  
Poonsuk Prasertsan ◽  
Sompong O-Thong

Background Anaerobic digestion (AD) is a suitable process for treating high moisture MSW with biogas and biofertilizer production. However, the low stability of AD performance and low methane production results from high moisture MSW due to the fast acidify of carbohydrate fermentation. The effects of organic loading and incineration fly ash addition as a pH adjustment on methane production from high moisture MSW in the single-stage AD and two-stage AD processes were investigated. Results Suitable initial organic loading of the single-stage AD process was 17 gVS L−1 at incineration fly ash (IFA) addition of 0.5% with methane yield of 287 mL CH4 g−1 VS. Suitable initial organic loading of the two-stage AD process was 43 gVS L−1 at IFA addition of 1% with hydrogen and methane yield of 47.4 ml H2 g−1 VS and 363 mL CH4 g−1 VS, respectively. The highest hydrogen and methane production of 8.7 m3 H2 ton−1 of high moisture MSW and 66.6 m3 CH4 ton−1 of high moisture MSW was achieved at organic loading of 43 gVS L−1 at IFA addition of 1% by two-stage AD process. Biogas production by the two-stage AD process enabled 18.5% higher energy recovery than single-stage AD. The 1% addition of IFA into high moisture MSW was useful for controlling pH of the two-stage AD process with enhanced biogas production between 87–92% when compared to without IFA addition. Electricity production and energy recovery from MSW using the coupled incineration with biogas production by two-stage AD process were 9,874 MJ ton−1 MSW and 89%, respectively. Conclusions The two-stage AD process with IFA addition for pH adjustment could improve biogas production from high moisture MSW, as well as reduce lag phase and enhance biodegradability efficiency. The coupled incineration process with biogas production using the two-stage AD process was suitable for the management of MSW with low area requirement, low greenhouse gas emissions, and high energy recovery.


2020 ◽  
pp. 125166
Author(s):  
Michal Sposob ◽  
Joo-Youn Nam ◽  
Jun-Gyu Park ◽  
Tae-Hoon Kim ◽  
Yuhoon Hwang ◽  
...  

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