The viscosifying power, degree of acetylation and molecular mass of the alginate produced by Azotobacter vinelandii in shake flasks are determined by the oxygen transfer rate

2011 ◽  
Vol 46 (1) ◽  
pp. 290-297 ◽  
Author(s):  
Peña C ◽  
E. Galindo ◽  
J. Büchs
Keyword(s):  
Molecular Mass ◽  
Oxygen Transfer ◽  
Transfer Rate ◽  
Azotobacter Vinelandii ◽  
Oxygen Transfer Rate ◽  
Degree Of Acetylation ◽  
Shake Flasks

Copolymerization of poly-(3-hydroxybutyrate-co-3-hydroxyvalerate) produced by Azotobacter vinelandii OPNA is determined by the oxygen transfer rate of the culture

2021 ◽  
Author(s):  
Angie Johanna Torres Pedraza ◽  
Holjes Salgado-Lugo ◽  
Daniel Segura ◽  
Carlos Pena
Keyword(s):  
Mutant Strain ◽  
Oxygen Transfer ◽  
Transfer Rate ◽  
Azotobacter Vinelandii ◽  
Molar Fraction ◽  
Oxygen Transfer Rate ◽  
Agitation Rate ◽  
Industrial Level ◽  
Maximum Content

Abstract The aim of this study was to evaluate the changes in the composition of poly-3-hydoxybutyrate-co-3-hydroxyvalerate (PHBV) by an OPNA mutant strain of Azotobacter vinelandii cultured under different conditions. In shaken flasks, the oxygen transfer rate (OTR) significantly affected the PHBV composition, the highest content of 3-hydroxyvalerate (3HV) (28%) was achieved from the cultures developed at high OTR max (5.87 mmol L -1 h -1 ). In the bioreactor, the cultures were grown under oxygen limited conditions and different OTRs, varying the agitation rate. The highest PHBV accumulation (85%) was achieved at low OTR max (4.96 mmol L -1 h -1 ), whereas, the maximum content of 3HV (8.5 mol%) was obtained at higher OTR max (20.3 mmol L -1 h -1 ). Our results reveal that increasing the OTR rises the molar fraction of 3HV in the PHBV copolymer. Therefore, the manipulation of OTR could be a feasible strategy to produce PHBV copolymers with different monomeric compositions at industrial level.

scholarly journals The metabolic switch can be activated in a recombinant strain of Streptomyces lividans by a low oxygen transfer rate in shake flasks

2018 ◽  
Vol 17 (1) ◽  
Author(s):  
Ramsés A. Gamboa-Suasnavart ◽  
Norma A. Valdez-Cruz ◽  
Gerardo Gaytan-Ortega ◽  
Greta I. Reynoso-Cereceda ◽  
Daniel Cabrera-Santos ◽  
...  
Keyword(s):  
Oxygen Transfer ◽  
Transfer Rate ◽  
Recombinant Strain ◽  
Oxygen Transfer Rate ◽  
Streptomyces Lividans ◽  
Low Oxygen ◽  
Metabolic Switch ◽  
Shake Flasks

Oxygen supply in disposable shake-flasks: prediction of oxygen transfer rate, oxygen saturation and maximum cell concentration during aerobic growth

2013 ◽  
Vol 35 (8) ◽  
pp. 1223-1230 ◽  
Author(s):  
Sarah Schiefelbein ◽  
Alexander Fröhlich ◽  
Gernot T. John ◽  
Falco Beutler ◽  
Christoph Wittmann ◽  
...  
Keyword(s):  
Oxygen Saturation ◽  
Oxygen Transfer ◽  
Transfer Rate ◽  
Oxygen Supply ◽  
Cell Concentration ◽  
Oxygen Transfer Rate ◽  
Aerobic Growth ◽  
Shake Flasks ◽  
Maximum Cell ◽  
Maximum Cell Concentration
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