Oxygen supply in disposable shake-flasks: prediction of oxygen transfer rate, oxygen saturation and maximum cell concentration during aerobic growth

2013 ◽  
Vol 35 (8) ◽  
pp. 1223-1230 ◽  
Author(s):  
Sarah Schiefelbein ◽  
Alexander Fröhlich ◽  
Gernot T. John ◽  
Falco Beutler ◽  
Christoph Wittmann ◽  
...  
Keyword(s):  
Oxygen Saturation ◽  
Oxygen Transfer ◽  
Transfer Rate ◽  
Oxygen Supply ◽  
Cell Concentration ◽  
Oxygen Transfer Rate ◽  
Aerobic Growth ◽  
Shake Flasks ◽  
Maximum Cell ◽  
Maximum Cell Concentration

Oxygen Supply Method Using Gas-Permeable Film for Wastewater Treatment

1993 ◽  
Vol 28 (7) ◽  
pp. 243-250 ◽  
Author(s):  
Y. Suzuki ◽  
S. Miyahara ◽  
K. Takeishi
Keyword(s):  
Wastewater Treatment ◽  
Energy Consumption ◽  
Oxygen Transfer ◽  
Transfer Rate ◽  
Oxygen Supply ◽  
Oxygen Transfer Rate ◽  
High Energy ◽  
High Rate ◽  
N Removal ◽  
High Energy Consumption

Gas-permeable film can separate air and water, and at the same time, let oxygen diffuse from the air to the water through the film. An oxygen supply method using this film was investigated for the purpose of reducing energy consumption for wastewater treatment. The oxygen transfer rate was measured for the cases with or without biofilm, which proved the high rate of oxygen transfer in the case with nitrifying biofilm which performed nitrification. When the Gas-permeable film with nitrifying biofilm was applied to the treatment of wastewater, denitrifying biofilm formed on the nitrifying biofilm, and simultaneous nitrification and denitrification occurred, resulting in the high rate of organic matter and T-N removal (7 gTOC/m2/d and 4 gT-N/m2/d, respectively). However, periodic sloughing of the denitrifying biofilm was needed to keep the oxygen transfer rate high. Energy consumption of the process using the film in the form of tubes was estimated to be less than 40% of that of the activated sludge process.

scholarly journals The metabolic switch can be activated in a recombinant strain of Streptomyces lividans by a low oxygen transfer rate in shake flasks

2018 ◽  
Vol 17 (1) ◽  
Author(s):  
Ramsés A. Gamboa-Suasnavart ◽  
Norma A. Valdez-Cruz ◽  
Gerardo Gaytan-Ortega ◽  
Greta I. Reynoso-Cereceda ◽  
Daniel Cabrera-Santos ◽  
...  
Keyword(s):  
Oxygen Transfer ◽  
Transfer Rate ◽  
Recombinant Strain ◽  
Oxygen Transfer Rate ◽  
Streptomyces Lividans ◽  
Low Oxygen ◽  
Metabolic Switch ◽  
Shake Flasks

scholarly journals Time-Resolved Monitoring of the Oxygen Transfer Rate of Chinese Hamster Ovary Cells Provides Insights Into Culture Behavior in Shake Flasks

2021 ◽  
Vol 9 ◽  
Author(s):  
Nina Ihling ◽  
Lara Pauline Munkler ◽  
Christoph Berg ◽  
Britta Reichenbächer ◽  
Johannes Wirth ◽  
...  
Keyword(s):  
Oxygen Transfer ◽  
Transfer Rate ◽  
Shake Flask ◽  
Oxygen Transfer Rate ◽  
Carbon Sources ◽  
Chinese Hamster ◽  
Respiration Activity ◽  
Time Resolved ◽  
Non Invasive ◽  
Shake Flasks

Cultivations of mammalian cells are routinely conducted in shake flasks. In contrast to instrumented bioreactors, reliable options for non-invasive, time-resolved monitoring of the culture status in shake flasks are lacking. The Respiration Activity Monitoring Respiration Activity Monitoring System system was used to determine the oxygen transfer rate (OTR) in shake flasks. It was proven that the OTR could be regarded as equal to the oxygen uptake rate as the change of the dissolved oxygen concentration in the liquid phase over time was negligibly small. Thus, monitoring the oxygen transfer rate (OTR) was used to increase the information content from shake flask experiments. The OTR of a Chinese hamster ovary cell line was monitored by applying electrochemical sensors. Glass flasks stoppered with cotton plugs and polycarbonate flasks stoppered with vent-caps were compared in terms of mass transfer characteristics and culture behavior. Similar mass transfer resistances were determined for both sterile closures. The OTR was found to be well reproducible within one experiment (standard deviation <10%). It correlated with changes in cell viability and depletion of carbon sources, thus, giving more profound insights into the cultivation process. Culture behavior in glass and polycarbonate flasks was identical. Monitoring of the OTR was applied to a second culture medium. Media differed in the maximum OTR reached during cultivation and in the time when all carbon sources were depleted. By applying non-invasive, parallelized, time-resolved monitoring of the OTR, the information content and amount of data from shake flask experiments was significantly increased compared to manual sampling and offline analysis. The potential of the technology for early-stage process development was demonstrated.

scholarly journals Investigation of poly(γ-glutamic acid) production via online determination of viscosity and oxygen transfer rate in shake flasks

2017 ◽  
Vol 11 (1) ◽  
Author(s):  
Lena Regestein née Meissner ◽  
Julia Arndt ◽  
Thomas G. Palmen ◽  
Tim Jestel ◽  
Hitoshi Mitsunaga ◽  
...  
Keyword(s):  
Glutamic Acid ◽  
Oxygen Transfer ◽  
Acid Production ◽  
Transfer Rate ◽  
Oxygen Transfer Rate ◽  
Shake Flasks ◽  
Glutamic Acid Production

DISTRIBUTION OF OXYGEN TRANSFER RATE IN STIRRED BIOREACTORS WITH SIMULATED BROTHS

2008 ◽  
Vol 7 (3) ◽  
pp. 199-211 ◽  
Author(s):  
Dan Cascaval ◽  
Anca-Irina Galaction ◽  
Stefanica Camarut ◽  
Radu Z. Tudose
Keyword(s):  
Oxygen Transfer ◽  
Transfer Rate ◽  
Oxygen Transfer Rate ◽  
Stirred Bioreactors

Estimation of oxygen transfer rate in sequencing batch reactor

1996 ◽  
Vol 34 (3-4) ◽  
pp. 413-420
Author(s):  
Y. C. Liao ◽  
D. J. Lee
Keyword(s):  
Dissolved Oxygen ◽  
Oxygen Transfer ◽  
Transfer Rate ◽  
Sequencing Batch Reactor ◽  
Batch Reactor ◽  
Oxygen Transfer Rate ◽  
Intermittent Aeration ◽  
Transient Effects ◽  
Operational Parameters ◽  
Transient Model

Transient model of oxygen transfer rate in a sequencing batch reactor is derived and solved numerically. The dissolved oxygen response under several conditions is analyzed. Effects of operational parameters and liquid bath height are studied. When with short, intermittent aeration periods, the transient effects on oxygen transfer rate may be substantial and should be taken into considerations. An example considering bioreaction is also given.

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