Combination of dilute acid and ionic liquid pretreatments of sugarcane bagasse for glucose by enzymatic hydrolysis

This work was aimed at investigating the effect of process parameters on dilute acid pretreatment and enzymatic hydrolysis of spent citronella biomass (after citronella oil extraction) and sugarcane bagasse on total reducing sugar (TRS) yield. In acid pretreatment, the parameters studied were acid concentration, temperature, and time. At the optimized condition (0.1 M H2SO4, 120°C, and 120 min), maximum TRS obtained was 452.27 mg·g−1and 487.50 mg·g−1for bagasse and citronella, respectively. Enzymatic hydrolysis of the pretreated biomass usingTrichoderma reesei26291 showed maximum TRS yield of 226.99 mg·g−1for citronella and 282.85 mg·g−1for bagasse at 10 FPU, 50°C, and 48 hr. The maximum crystallinity index (CI) of bagasse and citronella after acid pretreatment obtained from X-ray diffraction analysis was 64.41% and 56.18%, respectively. Decreased CI after enzymatic hydrolysis process to 37.28% and 34.16% for bagasse and citronella, respectively, revealed effective conversion of crystalline cellulose to glucose. SEM analysis of the untreated and treated biomass revealed significant hydrolysis of holocellulose and disruption of lignin.

Download Full-text

Fractionation of Sugarcane Bagasse Using a Combined Process of Dilute Acid and Ionic Liquid Treatments

Applied Biochemistry and Biotechnology ◽

10.1007/s12010-012-9742-4 ◽

2012 ◽

Vol 167 (7) ◽

pp. 1921-1937 ◽

Cited By ~ 13

Author(s):

Danie Diedericks ◽

Eugéne van Rensburg ◽

Johann F. Görgens

Keyword(s):

Ionic Liquid ◽

Sugarcane Bagasse ◽

Dilute Acid ◽

Combined Process

Download Full-text

Comparison of lignin yield from sugarcane bagasse pellets using liquid hot water and ionic liquid pretreatment methods

10.51415/10321/3609 ◽

2019 ◽

Author(s):

◽

Gueh Charles Gnana

Keyword(s):

Ionic Liquid ◽

Ionic Liquids ◽

Reaction Time ◽

Enzymatic Hydrolysis ◽

Sugarcane Bagasse ◽

Microwave Digestion ◽

Hot Water ◽

Time Intervals ◽

Liquid Hot Water ◽

Hydrogen Sulphate

In this research work, lignin yield from sugarcane bagasse pellets (SBP) was investigated after treatment of sugar cane bagasse with liquid hot water (LHW) and enzymatic hydrolysis followed by ionic liquids (ILs) and only ionic liquids pretreatment methods. In the LHW and ionic liquid methods, the SBP were first treated with LHW at 200 °C, for 30 minutes in a suitable reactor, for removal of hemicellulose. The complex cellulignin residue was treated separately with either of two ionic liquids namely: 1-ethyl-3- methylimidazolium acetate ([Emim][OAc]) or 1-butyl-3- methylimidazolium hydrogen sulphate ([Bmim][HSO4]), using microwave digestion at varying time intervals. Theionicliquidmethodinvolvedthepretreatmentofsugarcanebagasse pelletswith either 1-ethyl-3-methylimidazolium acetate or 1-butyl-3-methylimidazolium hydrogen sulphate followed by microwave digestion at varying time intervals. Ultraviolet (UV) spectroscopy at a wavelength of 280 nm was used as a tool for quantification of lignin. The different functional groups of the extracted lignin were confirmed using attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy. Thermogravimetric analysis (TGA) provided information on thermal characteristics of the extracted lignin. In addition to material characterization, mixed factorial ANOVA was performed to compare the extracted lignin yield using the LHW and IL and the ionic liquid pretreatment methods. High performance liquid chromatography (HPLC) was used to identify the C5 sugars in the hydrolysate after LHW pretreatment. X-ray diffraction (XRD) was used to identify cellulose peaks of cellulignin and SBP and ILs treated samples. The results indicated that the lignin yield from sugarcane bagasse pellets after liquid hot water treatment and enzymatic hydrolysis was 37.8 % (m/v). The highest percentage yield of lignin extracted from the complex cellulignin (LHW and IL) was found to be 68.00 % (m/v) and 32.04 % (m/v) for [Emim][OAc] and [Bmim][HSO4], respectively for the optimized reaction time of 10 minutes. However, 67.25 % (m/v) and 48.94 % (m/v) of the extracted lignin were obtained for the pretreated SBP with [Emim][OAc] and [Bmim][HSO4], respectively for a reaction time of 20 minutes. This comparative study revealed that, there is no significant difference between the yield of lignin extracted from the complex cellulignin (68.00%) and sugarcane bagasse pellets (67.25 %).The sugarcane bagasse pellets is the preferred method since it doesn’t require high energy input.

Download Full-text