scholarly journals Comparison of lignin yield from sugarcane bagasse pellets using liquid hot water and ionic liquid pretreatment methods

2019 ◽  
Author(s):  
◽  
Gueh Charles Gnana
Keyword(s):  
Ionic Liquid ◽  
Ionic Liquids ◽  
Reaction Time ◽  
Enzymatic Hydrolysis ◽  
Sugarcane Bagasse ◽  
Microwave Digestion ◽  
Hot Water ◽  
Time Intervals ◽  
Liquid Hot Water ◽  
Hydrogen Sulphate

In this research work, lignin yield from sugarcane bagasse pellets (SBP) was investigated after treatment of sugar cane bagasse with liquid hot water (LHW) and enzymatic hydrolysis followed by ionic liquids (ILs) and only ionic liquids pretreatment methods. In the LHW and ionic liquid methods, the SBP were first treated with LHW at 200 °C, for 30 minutes in a suitable reactor, for removal of hemicellulose. The complex cellulignin residue was treated separately with either of two ionic liquids namely: 1-ethyl-3- methylimidazolium acetate ([Emim][OAc]) or 1-butyl-3- methylimidazolium hydrogen sulphate ([Bmim][HSO4]), using microwave digestion at varying time intervals. Theionicliquidmethodinvolvedthepretreatmentofsugarcanebagasse pelletswith either 1-ethyl-3-methylimidazolium acetate or 1-butyl-3-methylimidazolium hydrogen sulphate followed by microwave digestion at varying time intervals. Ultraviolet (UV) spectroscopy at a wavelength of 280 nm was used as a tool for quantification of lignin. The different functional groups of the extracted lignin were confirmed using attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy. Thermogravimetric analysis (TGA) provided information on thermal characteristics of the extracted lignin. In addition to material characterization, mixed factorial ANOVA was performed to compare the extracted lignin yield using the LHW and IL and the ionic liquid pretreatment methods. High performance liquid chromatography (HPLC) was used to identify the C5 sugars in the hydrolysate after LHW pretreatment. X-ray diffraction (XRD) was used to identify cellulose peaks of cellulignin and SBP and ILs treated samples. The results indicated that the lignin yield from sugarcane bagasse pellets after liquid hot water treatment and enzymatic hydrolysis was 37.8 % (m/v). The highest percentage yield of lignin extracted from the complex cellulignin (LHW and IL) was found to be 68.00 % (m/v) and 32.04 % (m/v) for [Emim][OAc] and [Bmim][HSO4], respectively for the optimized reaction time of 10 minutes. However, 67.25 % (m/v) and 48.94 % (m/v) of the extracted lignin were obtained for the pretreated SBP with [Emim][OAc] and [Bmim][HSO4], respectively for a reaction time of 20 minutes. This comparative study revealed that, there is no significant difference between the yield of lignin extracted from the complex cellulignin (68.00%) and sugarcane bagasse pellets (67.25 %).The sugarcane bagasse pellets is the preferred method since it doesn’t require high energy input.

scholarly journals The Effect of the Chemical Character of Ionic Liquids on Biomass Pre-Treatment and Posterior Enzymatic Hydrolysis

Molecules ◽  
2019 ◽  
Vol 24 (4) ◽  
pp. 808 ◽  
Author(s):  
Joana Bernardo ◽  
Francisco Gírio ◽  
Rafał Łukasik
Keyword(s):  
Hydrogen Bond ◽  
Ionic Liquid ◽  
Ionic Liquids ◽  
Enzymatic Hydrolysis ◽  
Wheat Straw ◽  
Crystalline Cellulose ◽  
Fractionation Process ◽  
Hydrogen Sulphate ◽  
Chemical Character ◽  
Pre Treatment

Ionic liquids have been recognised as interesting solvents applicable in efficient lignocellulosic biomass valorisation, especially in biomass fractionation into individual polymeric components or direct hydrolysis of some biomass fractions. Considering the chemical character of ionic liquids, two different approaches paved the way for the fractionation of biomass. The first strategy integrated a pre-treatment, hydrolysis and conversion of biomass through the employment of hydrogen-bond acidic 1-ethyl-3-methyimidazolim hydrogen sulphate ionic liquid. The second strategy relied on the use of a three-step fractionation process with hydrogen-bond basic 1-ethyl-3-methylimidazolium acetate to produce high purity cellulose, hemicellulose and lignin fractions. The proposed approaches were scrutinised for wheat straw and eucalyptus residues. These different biomasses enabled an understanding that enzymatic hydrolysis yields are dependent on the crystallinity of the pre-treated biomass. The use of acetate based ionic liquid allowed crystalline cellulose I to change to cellulose II and consequently enhanced the glucan to glucose yield to 93.1 ± 4.1 mol% and 82.9 ± 1.2 mol% for wheat straw and eucalyptus, respectively. However, for hydrogen sulphate ionic liquid, the same enzymatic hydrolysis yields were 61.6 ± 0.2 mol% for wheat straw and only 7.9 ± 0.3 mol% for eucalyptus residues. These results demonstrate the importance of both ionic liquid character and biomass type for efficient biomass processing.

Enhancement of enzyme hydrolysis by increasing the zeta potential to reduce non-productive cellulase adsorption on sugarcane bagasse treated with liquid hot water

BioResources ◽  
2020 ◽  
Vol 15 (3) ◽  
pp. 5965-5974
Author(s):  
Tianqing Lan ◽  
Tong Lin ◽  
Yuyue Qin
Keyword(s):  
Enzymatic Hydrolysis ◽  
Zeta Potential ◽  
Sugarcane Bagasse ◽  
Binding Sites ◽  
Surface Hydrophobicity ◽  
Repulsive Force ◽  
Hot Water ◽  
Enzyme Hydrolysis ◽  
Cellulase Adsorption ◽  
Liquid Hot Water

The enhancement of enzymatic hydrolysis is important for the biorefinery industry of lignocellulose. Changing the pH of hydrolysis is a simple and direct way to improve hydrolysis efficiencies. In this study, the enzymatic hydrolysis efficiencies of sugarcane bagasse (SCB) treated with liquid hot water (LHW) were 56.7% and 65.5% at pHs of 4.8 and 5.5, respectively. The result of cellulase adsorption on the LHW treated SCB showed that the non-productive adsorption was smaller at pH 5.5, which might tend to enhance hydrolysis. The surface hydrophobicity of lignin was larger at pH 5.5. This suggested that the hydrophobic interaction was not dominant because a strong hydrophobicity force can cause more non-productive adsorption of cellulase with lignin. At pH 5.5, the surface negative charges of lignin and cellulase increased. Therefore, the electrostatic repulsive force between lignin and cellulase increased, leading to less of the non-productive adsorption of cellulase on lignin. In addition, the cellulase desorption from the LHW treated SCB also increased at pH 5.5. This was beneficial in increasing the possibility of cellulase re-adsorption in new binding sites on cellulose and promoting enzyme hydrolysis efficiency.

The Ways of Factors Influencing High-Solid Enzymatic Hydrolysis of Sugarcane Bagasse Treated by Liquid Hot Water

2017 ◽  
Vol 2 (22) ◽  
pp. 6240-6244 ◽  
Author(s):  
Wen Wang ◽  
Qiong Wang ◽  
Xuesong Tan ◽  
Wei Qi ◽  
Qiang Yu ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Sugarcane Bagasse ◽  
Hot Water ◽  
High Solid ◽  
Factors Influencing ◽  
Liquid Hot Water ◽  
Hydrolysis Of

scholarly journals Liquid Hot Water Pretreatment and Enzymatic Hydrolysis as a Valorization Route of Italian Green Pepper Waste to Delivery Free Sugars

Foods ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 1640
Author(s):  
M.A. Martín-Lara ◽  
L. Chica-Redecillas ◽  
A. Pérez ◽  
G. Blázquez ◽  
G. Garcia-Garcia ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Liquid Fraction ◽  
Hot Water ◽  
Effect Of Temperature ◽  
Green Pepper ◽  
Free Sugars ◽  
Liquid Hot Water Pretreatment ◽  
Liquid Hot Water ◽  
Water Pretreatment ◽  
Hot Water Pretreatment

In this work, liquid hot water pretreatment (autohydrolysis) was used to improve enzymatic hydrolysis of a commonly consumed vegetable waste in Spain, Italian green pepper, to finally produce fermentable sugars. Firstly, the effect of temperature and contact time on sugar recovery during pretreatment (in insoluble solid and liquid fraction) was studied in detail. Then, enzymatic hydrolysis using commercial cellulase was performed with the insoluble solid resulting from pretreatment. The objective was to compare results with and without pretreatment. The results showed that the pretreatment step was effective to facilitate the sugars release in enzymatic hydrolysis, increasing the global sugar yield. This was especially notable when pretreatment was carried out at 180 °C for 40 min for glucose yields. In these conditions a global glucose yield of 61.02% was obtained. In addition, very low concentrations of phenolic compounds (ranging from 69.12 to 82.24 mg/L) were found in the liquid fraction from enzymatic hydrolysis, decreasing the possibility of fermentation inhibition produced by these components. Results showed that Italian green pepper is an interesting feedstock to obtain free sugars and prevent the enormous quantity of this food waste discarded annually.

Combination of dilute acid and ionic liquid pretreatments of sugarcane bagasse for glucose by enzymatic hydrolysis

2013 ◽  
Vol 48 (12) ◽  
pp. 1942-1946 ◽  
Author(s):  
Li-Qun Jiang ◽  
Zhen Fang ◽  
Xing-Kang Li ◽  
Jia Luo ◽  
Suet-Pin Fan
Keyword(s):  
Ionic Liquid ◽  
Enzymatic Hydrolysis ◽  
Sugarcane Bagasse ◽  
Dilute Acid

scholarly journals Synergistic effects of metal salt and ionic liquid on the pretreatment of sugarcane bagasse for enhanced enzymatic hydrolysis

2018 ◽  
Vol 249 ◽  
pp. 1058-1061 ◽  
Author(s):  
Jiaxing Xu ◽  
Jiming Xu ◽  
Sen Zhang ◽  
Jun Xia ◽  
Xiaoyan Liu ◽  
...  
Keyword(s):  
Ionic Liquid ◽  
Enzymatic Hydrolysis ◽  
Sugarcane Bagasse ◽  
Metal Salt ◽  
Synergistic Effects

scholarly journals Enzymatic hydrolysis of chicken viscera proteins in the presence of an ionic liquid as a strategy to improve the antioxidant properties of the hydrolysates

2019 ◽  
Author(s):  
Vitor Geniselli da Silva ◽  
Ruann Janser Soares de Castro
Keyword(s):  
Ionic Liquid ◽  
Ionic Liquids ◽  
Enzymatic Hydrolysis ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Protein Hydrolysates ◽  
Tetramethylammonium Bromide ◽  
Chicken Viscera ◽  
Hydrolysis Of

Aiming to explore the use of ionic liquids (ILs) not yet described in the literature, this work evaluated the hydrolysis of proteins from chicken viscera using the protease Alcalase modified and unmodified by the IL tetramethylammonium bromide. The protein hydrolysates produced in the presence of the IL presented values of antioxidant activities 40% higher than the hydrolysates obtained without IL. In addition, with the presence of the IL, it was possible to obtain protein hydrolysates from chicken viscera with similar antioxidant activities, compared to the protein hydrolysates produced without IL, using 1/3 of the amount of enzyme.

Tissue-specific biomass recalcitrance in corn stover pretreated with liquid hot-water: Enzymatic hydrolysis (part 1)

2011 ◽  
Vol 109 (2) ◽  
pp. 390-397 ◽  
Author(s):  
Meijuan Zeng ◽  
Eduardo Ximenes ◽  
Michael R. Ladisch ◽  
Nathan S. Mosier ◽  
Wilfred Vermerris ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Corn Stover ◽  
Hot Water ◽  
Biomass Recalcitrance ◽  
Tissue Specific ◽  
Liquid Hot Water ◽  
Specific Biomass
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