Set Potential Determination for Rice Tungro Spherical Virus (RTSV) Detection Using Chronoamperometry Measurement

Infection of viruses in plants often modifies plant responses to biotic and abiotic stresses. In the present study we examined the effects of Rice tungro spherical virus (RTSV) infection on drought response in rice. RTSV infection delayed the onset of leaf rolling by 1–2 days. During the delay in drought response, plants infected with RTSV showed higher stomatal conductance and less negative leaf water potential under drought than those of uninfected plants, indicating that RTSV-infected leaves were more hydrated. Other growth and physiological traits of plants under drought were not altered by infection with RTSV. An expression analysis of genes for drought response-related transcription factors showed that the expression of OsNAC6 and OsDREB2a was less activated by drought in RTSV-infected plants than in uninfected plants, further suggesting improved water status of the plants due to RTSV infection. RTSV accumulated more in plants under drought than in well-watered plants, indicating the increased susceptibility of rice plants to RTSV infection by drought. Collectively, these results indicated that infection with RTSV can transiently mitigate the influence of drought stress on rice plants by increasing leaf hydration, while drought increased the susceptibility of rice plants to RTSV.

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Molecular cloning and sequencing of coat protein-encoding cDNA of rice tungro spherical virus—A plant picornavirus

Virus Genes ◽

10.1007/bf01702392 ◽

1993 ◽

Vol 7 (2) ◽

pp. 121-132 ◽

Cited By ~ 14

Author(s):

Shulu Zhang ◽

Matthew C. Jones ◽

Pat Barker ◽

Jeffrey W. Davies ◽

Roger Hull

Keyword(s):

Coat Protein ◽

Molecular Cloning ◽

Rice Tungro Spherical Virus ◽

Cloning And Sequencing ◽

Protein Encoding

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Gene expression responses to Rice tungro spherical virus in susceptible and resistant near-isogenic rice plants

Virus Research ◽

10.1016/j.virusres.2012.11.003 ◽

2013 ◽

Vol 171 (1) ◽

pp. 111-120 ◽

Cited By ~ 11

Author(s):

Kouji Satoh ◽

Hiroaki Kondoh ◽

Teresa B. De Leon ◽

Reena Jesusa A. Macalalad ◽

Rogelio C. Cabunagan ◽

...

Keyword(s):

Gene Expression ◽

Rice Tungro Spherical Virus ◽

Rice Plants

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Coat protein 3 of Rice tungro spherical virus is the key target gene for development of RNAi mediated tungro disease resistance in rice

Agri Gene ◽

10.1016/j.aggene.2019.100084 ◽

2019 ◽

Vol 12 ◽

pp. 100084 ◽

Cited By ~ 1

Author(s):

P. Malathi ◽

Shaik Abdul Muzammil ◽

D. Krishnaveni ◽

S.M. Balachandran ◽

Satendra K. Mangrauthia

Keyword(s):

Disease Resistance ◽

Coat Protein ◽

Target Gene ◽

Rice Tungro Spherical Virus

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Transfer of transgenes for resistance to rice tungro disease into high-yielding rice cultivars through gene-based marker-assisted selection

The Journal of Agricultural Science ◽

10.1017/s0021859611000827 ◽

2012 ◽

Vol 150 (5) ◽

pp. 610-618 ◽

Cited By ~ 9

Author(s):

S. ROY ◽

A. BANERJEE ◽

J. TARAFDAR ◽

B. K. SENAPATI ◽

I. DASGUPTA

Keyword(s):

Marker Assisted Selection ◽

Rice Tungro Bacilliform Virus ◽

35S Promoter ◽

Rice Cultivars ◽

Rice Tungro Spherical Virus ◽

Challenge Inoculation ◽

Rice Line ◽

Rice Tungro Disease ◽

Sense Orientation ◽

Simultaneous Infection

SUMMARYRice tungro disease (RTD), caused by the simultaneous infection of rice tungro bacilliform virus (RTBV) and rice tungro spherical virus (RTSV), is one of the major threats to sustainable rice production in South and Southeast Asia. Transgenic resistance against RTBV has been reported previously using an RNA interference (RNAi) construct (ORF IV of RTBV, placed both in sense and anti-sense orientation under CaMV 35S promoter), in the scented rice line Pusa Basmati-1 (PB-1). This construct was transferred to two high-yielding tungro-susceptible indica rice cultivars (IET4094 and IET4786) from the transgenic PB-1 rice line using back cross breeding till the BC2F3 stage. On challenge inoculation, the progenies (BC2F1) showed mild symptoms of tungro, in contrast to severe symptoms displayed by the recurrent parents. Segregation of the transgene indicated near homozygosity of the plants at the BC2F3 stage, implying that the lines can be used as a valuable resistance source for further breeding against RTD.

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Occurrence and Spread of Rice Tungro Spherical Virus in the Philippines

Plant Disease ◽

10.1094/pd-70-971 ◽

1986 ◽

Vol 70 (10) ◽

pp. 971 ◽

Cited By ~ 11

Author(s):

N. B. Bajet

Keyword(s):

The Philippines ◽

Rice Tungro Spherical Virus

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Near Immunity to Rice Tungro Spherical Virus Achieved in Rice by a Replicase-Mediated Resistance Strategy

Phytopathology ◽

10.1094/phyto.1999.89.11.1022 ◽

1999 ◽

Vol 89 (11) ◽

pp. 1022-1027 ◽

Cited By ~ 30

Author(s):

H. Huet ◽

S. Mahendra ◽

J. Wang ◽

E. Sivamani ◽

C. A. Ong ◽

...

Keyword(s):

Antisense Rna ◽

Rice Tungro Bacilliform Virus ◽

Japonica Rice ◽

Rice Tungro Spherical Virus ◽

Rice Plants ◽

Antisense Orientation ◽

Rice Tungro Disease ◽

Sense Orientation ◽

Moderate Resistance ◽

High Level

Rice tungro disease is caused by rice tungro bacilliform virus (RTBV), which is responsible for the symptoms, and rice tungro spherical virus (RTSV), which assists transmission of both viruses by leafhoppers. Transgenic japonica rice plants (Oryza sativa) were produced containing the RTSV replicase (Rep) gene in the sense or antisense orientation. Over 70% of the plants contained one to five copies of the Rep gene, with integration occurring at a single locus in most cases. Plants producing antisense sequences exhibited significant but moderate resistance to RTSV (60%); accumulation of antisense RNA was substantial, indicating that the protection was not of the homology-dependent type. Plants expressing the full-length Rep gene, as well as a truncated Rep gene, in the (+)-sense orientation were 100% resistant to RTSV even when challenged with a high level of inoculum. Accumulation of viral RNA was low, leading us to conclude that RTSV Rep-mediated resistance is not protein-mediated but is of the cosuppression type. Resistance was effective against geographically distinct RTSV isolates. In addition, RTSV-resistant transgenic rice plants were unable to assist transmission of RTBV. Such transgenic plants could be used in an epidemiological approach to combat the spread of the tungro disease.

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