Coat protein 3 of Rice tungro spherical virus is the key target gene for development of RNAi mediated tungro disease resistance in rice

Abstract microRNAs act as fine-tuners in the regulation of plant growth and resistance against biotic and abiotic stress. Here we demonstrate that rice miR1432 fine-tunes yield and blast disease resistance via different modules. The expression of miR1432 is differentially regulated in the susceptible and resistance accessions by the infection of the blast fungus Magnaporthe oryzae. Overexpression of miR1432 leads to compromised resistance and decreased yield, whereas blocking miR1432 using a target mimic of miR1432 results in enhanced resistance and yield. Moreover, miR1432 suppresses the expression of LOC_Os03g59790, which encodes an EF-hand family protein 1 (OsEFH1). Overexpression of OsEFH1 leads to enhanced rice resistance but decreased grain yield. Consistently, blocking miR1432 or overexpression of OsEFH1 improves pathogen/microbe-associated molecular pattern- triggered immunity. In contrast, overexpression of ACOT, a previously identified target gene of miR1432 involved in the regulation of rice yield traits, has no significant effects on rice blast disease resistance. Altogether, these results indicate that miR1432 balances yield and resistance via different target genes, and blocking miR1432 can simultaneously improve yield and resistance.

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Selection of glufosinate-resistant cotton lines (Gossypium hirsutum L.) among bar transgenic lines

The Journal of Agriculture and Development ◽

10.52997/jad.9.05.2020 ◽

2020 ◽

Vol 19 (05) ◽

pp. 71-79

Author(s):

Nha T. Nguyen

Keyword(s):

Disease Resistance ◽

Target Gene ◽

Transgenic Cotton ◽

Gene Copy ◽

Selected Lines ◽

Gossypium Hirsutum L ◽

Transgenic Lines ◽

Botanical Characteristics ◽

Biological Assessments ◽

Selection Of

Basta-herbicide was tested at a concentration of 0.6 kg ai./ha for confirming resistance of 116 bar transgenic T1 lines; many lines with tolerance were obtained. Evaluation of selected lines using PCR, the integration and expression of transgenes in genome of transgenic plants was determined by southern blot and northern blot techniques. The combination of molecular and biological assessments resulted in the selection of 5 lines, i.e., B1, B6, B9, B18, and BF17 contained 01 target-gene copy which expressed transcription activities and showed uniform growth and best tolerance to glufosinate. Two T2 transgenic cotton lines, i.e., B9 and BF17, carried one copy of the gene which transmitted to the next generation according to the Mendel's rules of inheritance. These transgenic lines were highly resistant to Basta herbicide at a concentration of 0.6 kg ai./ha and had no difference in botanical characteristics and disease resistance in comparison with original non-transgenic Coker310 cotton plant

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Large-scale mutagenesis directed at specific chromosomes in wheat

Genome ◽

10.1139/g00-101 ◽

2001 ◽

Vol 44 (1) ◽

pp. 45-49 ◽

Cited By ~ 2

Author(s):

Robert Koebner ◽

James Hadfield

Keyword(s):

Disease Resistance ◽

Large Scale ◽

Target Gene ◽

Yellow Rust ◽

Chemical Mutagenesis ◽

Loss Of Function ◽

Novel Approach ◽

Large Numbers ◽

Resistance Pathway ◽

Order Of Magnitude

A novel approach has been developed to allow for the efficient selection of loss-of-function wheat mutants in the M1 generation, following either physical or chemical mutagenesis. This has generated an order of magnitude increase in the efficiency of identification of mutants, and also greatly increases the likelihood that selected individuals reflect mutation events at the target locus, rather than at genes acting elsewhere in the disease resistance pathway. The approach relies only on prior knowledge of the chromosomal location of the target gene, and uses the polyploidy of wheat to construct populations for mutagenesis in which large numbers of individuals are hemizygous for the target gene. The idea is illustrated with the mass identification of mutants at three independent genes for race-specific resistance to yellow rust, and one gene for resistance to powdery mildew.Key words: disease resistance mutant, hemizygotes, loss-of-function mutant.

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Genetic variation of coat protein gene among the isolates of Rice tungro spherical virus from tungro-endemic states of the India

Virus Genes ◽

10.1007/s11262-011-0708-3 ◽

2012 ◽

Vol 44 (3) ◽

pp. 482-487 ◽

Cited By ~ 4

Author(s):

Satendra K. Mangrauthia ◽

P. Malathi ◽

Surekha Agarwal ◽

G. Ramkumar ◽

D. Krishnaveni ◽

...

Keyword(s):

Genetic Variation ◽

Coat Protein ◽

Coat Protein Gene ◽

Protein Gene ◽

Rice Tungro Spherical Virus

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Degradation of a Coat Protein of Rice Tungro Spherical Virus during Purification.

Japanese Journal of Phytopathology ◽

10.3186/jjphytopath.61.41 ◽

1995 ◽

Vol 61 (1) ◽

pp. 41-43 ◽

Cited By ~ 1

Author(s):

Gilda J. MIRANDA ◽

M.L.M. YAMBAO ◽

Pepito Q. CABAUATAN ◽

S. Raja VENKITESH ◽

Hiroki KOGANEZAWA

Keyword(s):

Coat Protein ◽

Rice Tungro Spherical Virus

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Biological screening and analysis of transgenic rice lines expressing coat protein gene(s) of rice tungro spherical virus

Rice Genetics Collection - Rice Genetics II ◽

10.1142/9789812814289_0100 ◽

2008 ◽

pp. 768-772

Author(s):

E. Sivamani ◽

P. Shen ◽

C. A. Ong ◽

G. Hamidah ◽

G. Hassan ◽

...

Keyword(s):

Coat Protein ◽

Transgenic Rice ◽

Coat Protein Gene ◽

Protein Gene ◽

Rice Tungro Spherical Virus ◽

Biological Screening

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Field Resistance of Coat Protein Transgenic Papaya to Papaya ringspot virus in Jamaica

Plant Disease ◽

10.1094/pd-89-0841 ◽

2005 ◽

Vol 89 (8) ◽

pp. 841-847 ◽

Cited By ~ 20

Author(s):

Paula Tennant ◽

M. H. Ahmad ◽

D. Gonsalves

Keyword(s):

Disease Resistance ◽

Coat Protein ◽

Carica Papaya ◽

Virus Disease ◽

Field Resistance ◽

Ringspot Virus ◽

Papaya Ringspot Virus ◽

Two Generations ◽

Transgenic Lines ◽

Severe Leaf

Transgenic papayas (Carica papaya) containing translatable coat protein (CPT) or nontranslatable coat protein (CPNT) gene constructs were evaluated over two generations for field resistance to Papaya ringspot virus in a commercial papaya growing area in Jamaica. Reactions of R0 CPT transgenic lines included no symptoms and mild or severe leaf and fruit symptoms. All three reactions were observed in one line and among different lines. Trees of most CPNT lines exhibited severe symptoms of infection, and some also showed mild symptoms. R1 offspring showed reactions previously observed with parental R0 trees; however, reactions not previously observed or a lower incidence of the reaction were also obtained. The transgenic lines appear to possess virus disease resistance that can be manipulated in subsequent generations for the development of a product with acceptable commercial performance.

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Rice miR1432 Fine-Tunes the Balance of Yield and Blast Disease Resistance via Different Modules

Rice ◽

10.1186/s12284-021-00529-1 ◽

2021 ◽

Vol 14 (1) ◽

Author(s):

Yan Li ◽

Ya-Ping Zheng ◽

Xin-Hui Zhou ◽

Xue-Mei Yang ◽

Xiao-Rong He ◽

...

Keyword(s):

Disease Resistance ◽

Target Gene ◽

Target Genes ◽

Rice Yield ◽

Blast Disease ◽

Biotic And Abiotic Stress ◽

Enhanced Resistance ◽

Molecular Pattern ◽

Family Protein ◽

Ef Hand

AbstractmicroRNAs act as fine-tuners in the regulation of plant growth and resistance against biotic and abiotic stress. Here we demonstrate that rice miR1432 fine-tunes yield and blast disease resistance via different modules. Overexpression of miR1432 leads to compromised resistance and decreased yield, whereas blocking miR1432 using a target mimic of miR1432 results in enhanced resistance and yield. miR1432 suppresses the expression of LOC_Os03g59790, which encodes an EF-hand family protein 1 (OsEFH1). Overexpression of OsEFH1 leads to enhanced rice resistance but decreased grain yield. Further study revealed that miR1432 and OsEFH1 are differentially responsive to chitin, a fungus-derived pathogen/microbe-associated molecular pattern (PAMP/MAMP). Consistently, blocking miR1432 or overexpression of OsEFH1 improves chitin-triggered immunity responses. In contrast, overexpression of ACOT, another target gene regulating rice yield traits, has no significant effects on rice blast disease resistance. Altogether, these results indicate that miR1432 balances yield and resistance via different target genes, and blocking miR1432 can simultaneously improve yield and resistance.

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