scholarly journals RETRACTED: Prediction and Identification of B-Cell Epitopes on Goose Parvovirus Non-structural Protein

2011 ◽  
Vol 8 ◽  
pp. 289-293
Author(s):  
Tian-fei Yu ◽  
Ming Li ◽  
Yuan Guo ◽  
Guang-jun Song ◽  
Rong-xiu Li ◽  
...  
Keyword(s):  
B Cell ◽  
Structural Protein ◽  
B Cell Epitopes ◽  
Goose Parvovirus

Localization of linear B-cell epitopes on goose parvovirus structural protein

2012 ◽  
Vol 145 (1-2) ◽  
pp. 522-526 ◽  
Author(s):  
Tian-fei Yu ◽  
Bo Ma ◽  
Ming-chun Gao ◽  
Jun-wei Wang
Keyword(s):  
B Cell ◽  
Structural Protein ◽  
B Cell Epitopes ◽  
Goose Parvovirus ◽  
Linear B

Identification of three linear B cell epitopes against non-structural protein 3ABC of FMDV using monoclonal antibodies

2019 ◽  
Vol 103 (19) ◽  
pp. 8075-8086
Author(s):  
Wei Liu ◽  
Junjun Shao ◽  
Danian Chen ◽  
Yanyan Chang ◽  
Huiyun Chang ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
B Cell ◽  
Structural Protein ◽  
B Cell Epitopes ◽  
Linear B

scholarly journals Identification and Characterization of novel mutants of Nsp13 Protein among Indian SARS-CoV-2 isolates.

2021 ◽  
Author(s):  
Deepa Kumari ◽  
Namrata Kumari ◽  
Sudhir Kumar ◽  
Prabhat Kumar Sinha ◽  
Shivendra Kumar Shahi ◽  
...  
Keyword(s):  
B Cell ◽  
Protein Function ◽  
Protein Sequences ◽  
Structural Protein ◽  
Immunological Parameters ◽  
B Cell Epitopes ◽  
Global Challenge ◽  
Indian Isolates ◽  
And Function ◽  
The Impact

SARS-CoV-2, the causative agent of COVID-19 has mutated rapidly which enabled them to adapt and evade the immune system of the host. Emerging SARS-CoV-2 variants with crucial mutations pose a global challenge in context of therapeutic drugs and vaccines being developed globally. There are currently no specific therapeutics or vaccines available to combat SARS-CoV-2 devastation. In view of this, the current study aimed to identify and characterize the mutations found in the Nsp13 of SARS-CoV-2 in Indian isolates. Non-structural protein, Nsp13 protein sequences from Indian isolates were analyzed by comparing with the first reported Severe acute respiratory syndrome Corona Virus-2 (SARS-CoV-2) protein sequence from Wuhan, China. Out of 825 Nsp13 protein sequences, a total of 38 mutations were observed among Indian isolates. Our data show that mutations in Nsp13 at various positions (H164Y, A237T, T214I, C309Y, S236I, P419S, V305E, G54S, H290Y, P53S, A308Y, and A308Y) have a significant impact on the protein's stability and flexibility. Also, the impact of Nsp13 mutations on the protein function were predicted based on PROVEAN score that includes 15 mutants as neutral and 23 mutants as deleterious effect. Furthermore, B-cell epitopes contributed by Nsp13 were identified using various predictive immunoinformatic tools. Immunological Parameters of Nsp13 such as antigenicity, allergenicity and toxicity were evaluated to predict the potential B-cell epitopes. The predicted peptide sequences were correlated with the observed mutants. Our predicted data showed that there are seven high rank linear epitopes as well as 18 discontinuous B-cell epitopes based on immunoinformatic tools. Moreover, it was observed that out of total 38 identified mutations among Indian SARS-CoV-2 Nsp13 protein, four mutant residues at position 142 (E142), 245 (H245), 247 (V247) and 419 (P419) are localised in the predicted B cell epitopic region. Altogether, the results of the present in-silico study might help to understand the impact of the identified mutations in Nsp13 protein on its stability, flexibility and function.

scholarly journals Comprehensive mapping of West Nile virus (WNV)- and Japanese encephalitis virus serocomplex-specific linear B-cell epitopes from WNV non-structural protein 1

2012 ◽  
Vol 93 (1) ◽  
pp. 50-60 ◽  
Author(s):  
En-Cheng Sun ◽  
Jing Zhao ◽  
Ni-Hong Liu ◽  
Tao Yang ◽  
Jian-Nan Ma ◽  
...  
Keyword(s):  
West Nile Virus ◽  
Immune Responses ◽  
B Cell ◽  
Japanese Encephalitis Virus ◽  
Japanese Encephalitis ◽  
Encephalitis Virus ◽  
Structural Protein ◽  
West Nile ◽  
B Cell Epitopes ◽  
Linear B

West Nile virus (WNV) non-structural protein 1 (NS1) elicits protective immune responses during infection of animals. WNV NS1-specific antibody responses can provide the basis for serological diagnostic reagents, so the antigenic sites in NS1 that are targeted by host immune responses need to be identified and the conservation of these sites among the Japanese encephalitis virus (JEV) serocomplex members also needs to be defined. The present study describes the mapping of linear B-cell epitopes in WNV NS1. We screened eight NS1-specific mAbs and antisera (polyclonal antibodies; pAbs) from mice immunized with recombinant NS1 for reactivity against 35 partially overlapping peptides covering the entire WNV NS1. The screen using mAbs identified four WNV-specific (including Kunjin virus) epitopes, located at aa 21–36, 101–116, 191–206 and 261–276 in WNV NS1. However, using pAbs, only three WNV-specific epitopes were identified, located at positions 101–116, 191–206 and 231–246. Two of these epitopes (aa 21–36 and 261–276) had different reactivity with mAbs and pAbs. The knowledge and reagents generated in this study have potential applications in differential diagnostics and epitope-based marker vaccine development for WNV and viruses of the JEV serocomplex.

scholarly journals RETRACTED: Prediction and Identification of B-Cell Epitopes on Goose Parvovirus Capsid Protein

2011 ◽  
Vol 8 ◽  
pp. 284-288
Author(s):  
Tian-fei Yu ◽  
Ming Li ◽  
Yuan Guo ◽  
Guang-jun Song ◽  
Rong-xiu Li ◽  
...  
Keyword(s):  
B Cell ◽  
Capsid Protein ◽  
B Cell Epitopes ◽  
Goose Parvovirus

scholarly journals Computational Approach for Predicting Common Epitopes in the VP1 Structural Protein of Enterovirus Serotypes EV-D68, EV-D70, and EV-A71

2021 ◽  
Author(s):  
Rihabe Boussettine ◽  
Yassine Kasmi ◽  
Najwa Hassou ◽  
Hlima Bessi ◽  
Moulay Mustapha Ennaji
Keyword(s):  
T Cell ◽  
B Cell ◽  
Cell Epitope ◽  
Human Enterovirus ◽  
T Cell Epitopes ◽  
Structural Protein ◽  
B Cell Epitopes ◽  
Study Results ◽  
Enterovirus Serotypes

The three human Enterovirus serotypes D-68, D-70, and A-71, are common pathogens that are transmitted by fecal-oral and aerosol routes. These positive RNA viruses were known to exhibit high levels of genetic diversity and variability. Currently, no vaccines are available to protect humans from these three serotypes. Therefore, efforts are needed for the development of a vaccine directed against heterologous viruses. In our study, an immunoinformatics approach is used to identify T- and B-cell epitopes that may help for the generation of a universal vaccine against EV-D70, EV-A71, and EV-D68. B and T cell epitopes were selected based on their length. As a result, 5 B cell epitopes and 18 T cell epitopes were predicted. Our B cell epitope prediction results showed that there are a number of linear regions. Position 150-170 was found to be the most immunogenic for the different strains. Regarding the epitopes of the T lymphocytes, the result of the interactions shows that 95% of the predicted epitopes are common between the 3 sequences and the 5 methods used. These results demonstrate the great immunogenic potential of these sequences and their capacities to trigger immune reactions in people with different HLA alleles. The “VFYDGFAGF” epitope is the most important and most immunogenic for triggering an immune response. Our study results allowed us to identify epitopes to be used in the development of cross-protection vaccines against the three Enterovirus serotypes. However, in vivo and in vitro studies are needed to assess the potential of the epitopes predicted by our study.

scholarly journals Linear B-Cell Epitopes in Human Norovirus GII.4 Capsid Protein Elicit Blockade Antibodies

Vaccines ◽  
2021 ◽  
Vol 9 (1) ◽  
pp. 52
Author(s):  
Hassan Moeini ◽  
Suliman Qadir Afridi ◽  
Sainitin Donakonda ◽  
Percy A. Knolle ◽  
Ulrike Protzer ◽  
...  
Keyword(s):  
B Cell ◽  
Capsid Protein ◽  
Immune Escape ◽  
Solvent Accessibility ◽  
Effective Vaccine ◽  
Blood Group Antigens ◽  
Human Norovirus ◽  
B Cell Epitopes ◽  
Blocking Rate ◽  
Linear B

Human norovirus (HuNoV) is the leading cause of nonbacterial gastroenteritis worldwide with the GII.4 genotype accounting for over 80% of infections. The major capsid protein of GII.4 variants is evolving rapidly, resulting in new epidemic variants with altered antigenic potentials that must be considered for the development of an effective vaccine. In this study, we identify and characterize linear blockade B-cell epitopes in HuNoV GII.4. Five unique linear B-cell epitopes, namely P2A, P2B, P2C, P2D, and P2E, were predicted on the surface-exposed regions of the capsid protein. Evolving of the surface-exposed epitopes over time was found to correlate with the emergence of new GII.4 outbreak variants. Molecular dynamic simulation (MD) analysis and molecular docking revealed that amino acid substitutions in the putative epitopes P2B, P2C, and P2D could be associated with immune escape and the appearance of new GII.4 variants by affecting solvent accessibility and flexibility of the antigenic sites and histo-blood group antigens (HBAG) binding. Testing the synthetic peptides in wild-type mice, epitopes P2B (336–355), P2C (367–384), and P2D (390–400) were recognized as GII.4-specific linear blockade epitopes with the blocking rate of 68, 55 and 28%, respectively. Blocking rate was found to increase to 80% using the pooled serum of epitopes P2B and P2C. These data provide a strategy for expanding the broad blockade potential of vaccines for prevention of NoV infection.

scholarly journals Comparison of Monoclonal Gammopathies Linked to Poliovirus or Coxsackievirus vs. Other Infectious Pathogens

Cells ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 438
Author(s):  
Jean Harb ◽  
Nicolas Mennesson ◽  
Cassandra Lepetit ◽  
Maeva Fourny ◽  
Margaux Louvois ◽  
...  
Keyword(s):  
Multiple Myeloma ◽  
B Cell ◽  
Monoclonal Gammopathy ◽  
Coat Proteins ◽  
Chronic Stimulation ◽  
B Cell Epitopes ◽  
Monoclonal Immunoglobulins ◽  
Self Antigen ◽  
Coxsackievirus B1 ◽  
Undetermined Significance

Chronic stimulation by infectious pathogens or self-antigen glucosylsphingosine (GlcSph) can lead to monoclonal gammopathy of undetermined significance (MGUS) and multiple myeloma (MM). Novel assays such as the multiplex infectious antigen microarray (MIAA) and GlcSph assays, permit identification of targets for >60% purified monoclonal immunoglobulins (Igs). Searching for additional targets, we selected 28 purified monoclonal Igs whose antigen was not represented on the MIAA and GlcSph assays; their specificity of recognition was then analyzed using microarrays consisting of 3760 B-cell epitopes from 196 pathogens. The peptide sequences PALTAVETG and PALTAAETG of the VP1 coat proteins of human poliovirus 1/3 and coxsackievirus B1/B3, respectively, were specifically recognized by 6/28 monoclonal Igs. Re-analysis of patient cohorts showed that purified monoclonal Igs from 10/155 MGUS/SM (6.5%) and 3/147 MM (2.0%) bound to the PALTAVETG or PALTAAETG epitopes. Altogether, PALTAV/AETG-initiated MGUS are not rare and few seem to evolve toward myeloma.

Sign in / Sign up

Export Citation Format

Share Document