A hybrid vision-based surface coverage measurement method for robotic inspection

The microstructure of polyacrylonitrile (PAN) beads for affinity chromatography bioseparations was studied by TEM of stained ultramicrotomed thin-sections. Microstructural aspects such as overall pore size distribution, the distribution of pores within the beads, and surface coverage of functionalized beads affect performance properties. Stereological methods are used to quantify the internal structure of these chromatographic supports. Details of the process for making the PAN beads are given elsewhere. TEM specimens were obtained by vacuum impregnation with a low-viscosity epoxy and sectioning with a diamond knife. The beads can be observed unstained. However, different surface functionalities can be made evident by selective staining. Amide surface coverage was studied by staining in vapor of a 0.5.% RuO4 aqueous solution for 1 h. RuO4 does not stain PAN but stains, amongst many others, polymers containing an amide moiety.

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A new mental illness stigma measurement method: Error choice

PsycEXTRA Dataset ◽

10.1037/e683152011-559 ◽

2011 ◽

Author(s):

Patrick Michaels ◽

Patrick Corrigan

Keyword(s):

Mental Illness ◽

Measurement Method ◽

Mental Illness Stigma ◽

Method Error

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Measurement method for eye opening estimation on digital signals

IEE Proceedings F Communications Radar and Signal Processing ◽

10.1049/ip-f-1.1987.0079 ◽

1987 ◽

Vol 134 (5) ◽

pp. 454

Author(s):

M. Kawashima

Keyword(s):

Measurement Method ◽

Digital Signals ◽

Eye Opening

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Further Evidence that Glycoprotein IIb-IIIa Mediates Platelet Spreading on Subendothelium

Thrombosis and Haemostasis ◽

10.1055/s-0038-1647484 ◽

1991 ◽

Vol 65 (02) ◽

pp. 202-205 ◽

Cited By ~ 37

Author(s):

Harvey J Weiss ◽

Vincet T Turitto ◽

Hans R Baumgartner

Keyword(s):

Platelet Adhesion ◽

Surface Coverage ◽

Rabbit Aorta ◽

Conclusive Evidence ◽

Initial Contact ◽

Single Exposure ◽

Vessel Segment ◽

Multiple Exposures ◽

Wall Interaction ◽

Platelet Spreading

SummaryIn order to explore further the mechanism by which glycoprotein GPIIb-IIIa promotes platelet vessel wall interaction, platelet adhesion to subendothelium was studied in an annular chamber in which subendothelium from rabbit aorta was exposed at a shear rate of 2,600 s−1 to blood from patients with thrombasthenia. Perfusions were conducted for each of 5 exposure times (1 ,2,3, 5 and 10 min), and the percent surface coverage of the vessel segment with platelets in the contact (C) and spread (S) stage was determined. Increased values of platelet contact (C) were obtained in thrombasthenia at all exposure times; this finding is consistent with a defect in platelet spreadirg, based on a previously described kinetic model of platelet attachment to subendothelium. According to this model of attachment, increased values of platelet contact (C) at a single exposure time may be indicative of either a defect in spreading (S) or initial contact (C), but multiple exposures will result in increased contact only for defects which are related to defectiye platelet spreading (s).The results obtained over a broad range of exposure times provide more conclusive evidence that GPIIb-IIIa mediates platelet spreading than those previously obtained at single exposure times.

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Automated Microdensitometry And Protein Assays As Measures For Platelet Adhesion And Aggregation On Collagen Coated Slides Under Controlled Flow Conditions

10.1055/s-0038-1665758 ◽

1979 ◽

Author(s):

R Muggli ◽

H Baumgartner ◽

Th Tschopp

Keyword(s):

Surface Coverage ◽

Platelet Surface ◽

En Face ◽

Uncoated Surface ◽

Unit Surface ◽

Perfusion Time ◽

Flowing Blood ◽

Controlled Flow ◽

Protein Assays ◽

Microscope Slides

Microscope slides were homogeneously coated over a length of 2 cm with a mixture of soluble and fibrillar collagen and exposed at 37°C and under laminar flow to citrated whole rabbit blood at a flow-rate of 100 ml/min. Surface coverage with platelets (adhesion) and platelet accumulations higher than about 5 μm in height (aggregation) were determined by automated microdensitometry of fuchsine stained ‘en face’ preparations. The platelet mass per unit surface was measured with a modified Lowry technique whose sensitivity was equivalent to 5×l05platelets. Platelet number, amount of protein and surface coverage with platelet accumulations correlated. After a perfusion time of 10 min thrombi up to 30 μm in height and oriented in the direction of flow had developed on the collagen coated area. Surface coverage with platelets was 75% and the amount of deposited protein 1.4 μg/mm2(2×l06platelets/mm2). On the uncoated surface single platelets predominated; the surface coverage was 20% and the density of platelets 8×104/mm2. Acetyl- salicylic acid at 100 μm decreased platelet aggregation by about 80% without affecting adhesion.The new parallel plate perfusion system offers rapid quantitation of platelet-surface and platelet-platelet interaction after exposure to flowing blood and iftay also be diagnostically useful.

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