Assessment of Variation in Morpho-Physiological Traits and Genetic Diversity in Relation to Submergence Tolerance of Five Indigenous Lowland Rice Landraces

Aims: The aim of the study was to determine the genetic diversity of twenty four Boro rice landraces using rice genome specific twelve well known SSR markers. Study Design: Genomic DNA extraction, PCR amplification, Polyacrylamide gel electrophoresis (PAGE) and data analysis-these steps were followed to perform the research work. Data was analysed with the help of following software; POWERMAKER version 3.25, AlphaEaseFC (Alpha Innotech Corporation) version 4.0. UPGMA dendrogram was constructed using MEGA 5.1 software. Place and Duration of Study: The study was conducted at the Genetic Resources and Seed Division (GRSD), Bangladesh Rice Research Institute (BRRI), Joydebpur, Gazipur, Bangladesh during the period of November 2017 to March 2018. Methodology: Simple Sequence Repeat (SSR) markers were used to assay 24 landraces of Boro rice collected from the Gene Bank of Bangladesh Rice Research Institute (BRRI). Results: A total fifty four (54) alleles were detected, out of which forty five (45) polymorphic alleles were identified. The Polymorphic Information Content (PIC) of SSR markers ranged from 0.08 (RM447) to 0.84 (RM206) with an average value of PIC = 0.49. Gene diversity ranges from 0.08 (RM447) to 0.86 (RM206) with an average value of 0.52. The RM206 marker can be considered as the best marker among the studied markers for 24 rice landraces. Dendrogram based on Nei’s genetic distance using Unweighted Pair Group Method of Arithmetic Mean (UPGMA) indicated the segregation of 24 genotypes into three main clusters. Conclusion: The result revealed that SSR markers are very effective tools in the study of genetic diversity and genetic relationships and this result can be conveniently used for further molecular diversity analysis of rice genotypes to identify diverse parent for the development of high yielding variety in rice.

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Morphophysiological Traits as Indicators of Genetic Diversity in Brazilian Populations of Jatropha curcas L. Grown Under Water Deficit

Journal of Agricultural Studies ◽

10.5296/jas.v8i3.16707 ◽

2020 ◽

Vol 8 (3) ◽

pp. 527

Author(s):

Leandro Dias da Silva ◽

Fábio Pinto Gomes ◽

Mateus Pires Barbosa ◽

Raul Antônio Araújo do Bonfim ◽

Mikaela Oliveira Souza ◽

...

Keyword(s):

Genetic Diversity ◽

Plant Height ◽

Principal Components ◽

Jatropha Curcas ◽

Biodiesel Production ◽

Physiological Traits ◽

Guaiacol Peroxidase ◽

Jatropha Curcas L ◽

Root Area ◽

Genetic Dissimilarity

Jatropha curcas L. (Euphorbiaceae) is a species grown in tropicalcountries and used for biodiesel production. Morphophysiological traits were analyzed to assess the genetic diversity in nine genotypes of J. curcas under deficit water. Seeds of plants from different populations, collected in diferente brazilian regions, were grown under two water regimes (100% and 50% tank capacity). Multivariate analysis was used to characterize genetic diversity. The UPGMA dendrogram built from the genetic distance group indicated the segregation of genotypes into five groups for growth traits and six groups for physiological traits. Then, an principal components analysis was carried out, to evaluate the pattern of character variations and then segregate the characteristics that could distinguish parental genotypes for use in plant breeding. Results showed 65.50% and 56.02% for the two first principal components of growth and physiological traits, respectively. Total root area was the most determining trait for genetic dissimilarity (18.9%) and group formation, followed by plant height (17.9%) and number of leaves (17.6%). On the other hand, stomatal conductance (gs) (24.9%) and guaiacol peroxidase (GPX) (20.9%) were the most determining physiological traits. The analysis of morphophysiological traits indicated CNPAE-298 and 299 as the most distant genotypes among the plant groups. Under water-limited conditions, total root area, plant height, gs and GPX were the most efficient traits to explain genetic dissimilarity among the genotypes, and for this reason they should be referred as a priority for further studies on genotypes selection in this species.

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Interaction of submergence tolerance and drought yield QTLs ( Sub1 and qDTYs ) enhances morpho‐physiological traits and survival of rice ( Oryza sativa L.) under submergence

Annals of Applied Biology ◽

10.1111/aab.12664 ◽

2020 ◽

Author(s):

Asmuni Mohd Ikmal ◽

Abd Aziz Shamsudin Noraziyah ◽

Ratnam Wickneswari ◽

Ismail Amira ◽

Zulkafli Puteri Dinie Ellina

Keyword(s):

Oryza Sativa ◽

Oryza Sativa L ◽

Physiological Traits ◽

Submergence Tolerance

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Genetic diversity in the rice landraces (Oryza sativa L.) of coastal Sundarbans (India) and their adaptation to the local saline condition investigated both at molecular and physiological level

Acta Physiologiae Plantarum ◽

10.1007/s11738-015-2046-x ◽

2016 ◽

Vol 38 (2) ◽

Cited By ~ 3

Author(s):

Rashmita Samal ◽

Pritesh Sundar Roy ◽

Amit Kumar Dash ◽

Gundimeda Jwala Narsimha Rao ◽

Srinivasan Bharathkumar ◽

...

Keyword(s):

Genetic Diversity ◽

Oryza Sativa ◽

Oryza Sativa L ◽

Saline Condition ◽

Physiological Level ◽

Rice Landraces ◽

Local Saline

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Analysis of Genetic Diversity and Population Structure of Some Bangladeshi Rice Landraces and HYV

Journal of Scientific Research ◽

10.3329/jsr.v4i3.10416 ◽

2012 ◽

Vol 4 (3) ◽

pp. 757-767 ◽

Cited By ~ 1

Author(s):

M. M. Hassan ◽

A. K. M. Shamsuddin ◽

M. M. Islam ◽

K. Khatun ◽

J. Halder

Keyword(s):

Genetic Diversity ◽

Population Structure ◽

Genetic Distance ◽

Gene Diversity ◽

Allele Number ◽

Rice Landraces ◽

Efficient Management ◽

Ssr Loci ◽

Pattern Of Variation ◽

Rice Improvement

Information on the patterns of genetic variation and population structure is essential for rational use and efficient management of germplasms. It helps in monitoring germplasm and can also be used to predict potential genetic gains. Therefore, in the present study genetic diversity of 59 rice genotypes were assessed using 8 simple sequence repeat (SSR) primers. By the DNA profiling, a total of 114 alleles were detected. Allele number per/locus ranged from 9 to 27, with an average of 14.25. Average polymorphism information content (PIC) value was 0.857 with lowest 0.767 to highest 0.857. Mean gene diversity over all SSR loci was 0.870 with a range from 0.792 to 0.948. Fst values for each locus varied from 0.071 to 0.262. Genetic distance between the variety pair ranged from 0.33 to 1.0. The lowest genetic distance was found between Rajashili and Kumragori (2). Cluster and principal coordinate analysis (PCoA) analysis revealed similar pattern of variation. Marker RM11300 was found most polymorphic and robust among the accessions and can be widely used for rice germplasm characterization. The exclusive variability and unique feature of germplasm found in this study can be a gateway for both domestic and global rice improvement.© 2012 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved.doi: http://dx.doi.org/10.3329/jsr.v4i3.10416 J. Sci. Res. 4 (3), 757-767 (2012)

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