Combining near-infrared radiance of vegetation and fluorescence spectroscopy to detect effects of abiotic changes and stresses

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pp. 112856
Author(s):  
Yelu Zeng ◽  
Min Chen ◽  
Dalei Hao ◽  
Alexander Damm ◽  
Grayson Badgley ◽  
...  
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Mei Yang ◽  
Tongmei Ma ◽  
B.W. Li ◽  
Man-Chor Chan ◽  
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2021 ◽  
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Jens Petter Wold ◽  
Rune Rødbotten ◽  
Katinka Riiser Dankel ◽  
Nils Kristian Afseth

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Vol 40 (9-10) ◽  
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Abstract A series of pigments of the bacteriopheophytin a spectral type have been isolated from ageing cultures of Rhodopseudomonas sphaeroides strain R26. These pigments are formed in varying amounts, and can be most readily analyzed in vivo by their absorption in the 530 nm spectral range. They are enriched in sedimenting cells, but their formation is not affected by light. By chromatographic comparison with authentic pigments and chemical correlation, the following pigments have been identified: bacteriopheophytin a, bacteriopheophorbide a (which is the predominant product), pyrobacteriopheophorbide a and a fourth, very polar bacteriopheophytin a-type product of unknown structure. The major portion of these newly formed pigments is present in the cells in a state, in which the near-infrared absorption band is shifted to longer wavelengths. As shown by low temperature fluorescence spectroscopy, these forms are very similar to bacteriopheophorbide a aggregates.


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