Phylogenetic analysis of the genus Anaplasma in Southwestern China based on 16S rRNA sequence

2010 ◽  
Vol 89 (2) ◽  
pp. 262-265 ◽  
Author(s):  
Zuoyong Zhou ◽  
Kui Nie ◽  
Cheng Tang ◽  
Zhiying Wang ◽  
Rongqiong Zhou ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
16S Rrna ◽  
Southwestern China ◽  
Rrna Sequence ◽  
16S Rrna Sequence

scholarly journals Taxonomy and antimicrobial activity of streptosporangium strain SG163 isolated from a Saharan soil

2019 ◽  
Vol 20 (1) ◽  
pp. 35-54
Author(s):  
K. Bouti ◽  
◽  
H. Boudjella ◽  
N. Bouras ◽  
A. Zitouni ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Phylogenetic Analysis ◽  
16S Rrna ◽  
Culture Media ◽  
Antibacterial Activities ◽  
Antimicrobial Activities ◽  
Rrna Sequence ◽  
16S Rrna Sequence ◽  
Saharan Soil ◽  
Type Strains

n a continuing search fornew antimicrobial products from actinobacteriacollected in Algerian Saharan soils, an isolateof actinobacteria,designated Sg163, was selected for its interesting antimicrobial activity.The isolate wasidentified to the genus Streptosporangium by phenotypicand molecular criteria. The cultural and physiological characterizations as well as phylogenetic analysis indicated that the isolate was different from known members of the genus Streptosporangium. Analysis of the 16S rRNA sequence showed 97.09to 98.27% similarity with those of Streptosporangium type strains. The strainSg163 produced antifungal and antibacterial activities on several culture media. The highest antimicrobial activities were obtained in ISP2 medium. Three active products C1, C2 and C3 with both antifungal and antibacterial activities were isolated and purified by chromatographic methodswith C2, as the major compound. The data of the infraredspectroscopy andthe chemical revelations, suggested that the active molecules were glycosylatedaromaticcompounds.

scholarly journals 16S rRNA Sequence-based Rapid and Sensitive Detection of Aquatic Bacteria by On-chip Hybridization Following Multiplex PCR

2008 ◽  
Vol 54 (1) ◽  
pp. 123-128 ◽  
Author(s):  
Tomoaki Ichijo ◽  
Nobuyasu Yamaguchi ◽  
Katsuji Tani ◽  
Masao Nasu
Keyword(s):  
16S Rrna ◽  
Multiplex Pcr ◽  
Sensitive Detection ◽  
Rrna Sequence ◽  
16S Rrna Sequence ◽  
Aquatic Bacteria ◽  
On Chip

A new sand frog from Namaqualand, South Africa (Pyxicephalidae: Tomopterna)

Zootaxa ◽  
2019 ◽  
Vol 4609 (2) ◽  
pp. 225
Author(s):  
LYLE WILSON ◽  
ALAN CHANNING
Keyword(s):  
South Africa ◽  
New Species ◽  
16S Rrna ◽  
Advertisement Call ◽  
Rrna Sequence ◽  
16S Rrna Sequence ◽  
Adult Morphology ◽  
Haplotype Networks ◽  
Tyr Gene

Tomopterna branchi sp. nov. is described from Namaqualand, South Africa. It differs from all other Tomopterna species by advertisement call, 16S rRNA sequence and consistent differences in adult morphology. The tadpole is similar to that of Tomopterna cryptotis. Haplotype networks of 16S and the nuclear tyr gene show that it is distinct from T. delalandii, with which it has been confused. A phylogeny of the genus, excluding the little-known T. monticola, shows that the new species is basal to a clade that includes T. delalandii and six other species. We extend the known range of T. damarensis to southern Namibia, and correct the identification of some GenBank material. 

scholarly journals Mycobacterium neoaurumBacteremia in a Hemodialysis Patient

2003 ◽  
Vol 14 (1) ◽  
pp. 45-48 ◽  
Author(s):  
Marissa L Becker ◽  
Amar A Suchak ◽  
Joyce N Wolfe ◽  
Ryan Zarychanski ◽  
Amin Kabani ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
16S Rrna ◽  
Hemodialysis Patient ◽  
Immunocompromised Host ◽  
Blood Cultures ◽  
Clinical Suspicion ◽  
Diabetic Woman ◽  
Rrna Sequence ◽  
16S Rrna Sequence ◽  
16S Rrna Sequence Analysis

Bacteremia due toMycobacterium neoaurum, a rapidly growing mycobacterium, is described in a diabetic woman on hemodialysis. This is the first reported case of M neoaurum bacteremia in Canada. The organism initially grew on standard BacT/Alert SA aerobic blood cultures, and was subsequently positively identified using 16S rRNA sequence analysis. The present case serves to reinforce the need for a high index of clinical suspicion of infections caused by unusual microorganisms in the context of an immunocompromised host.

scholarly journals Thermocrinis ruber gen. nov., sp. nov., a Pink-Filament-Forming Hyperthermophilic Bacterium Isolated from Yellowstone National Park

1998 ◽  
Vol 64 (10) ◽  
pp. 3576-3583 ◽  
Author(s):  
Robert Huber ◽  
Wolfgang Eder ◽  
Stefan Heldwein ◽  
Gerhard Wanner ◽  
Harald Huber ◽  
...  
Keyword(s):  
16S Rrna ◽  
Yellowstone National Park ◽  
National Park ◽  
Carbon Sources ◽  
Culture Conditions ◽  
The Novel ◽  
Rrna Sequence ◽  
16S Rrna Sequence ◽  
Hyperthermophilic Bacterium ◽  
Outflow Channel

ABSTRACT A novel hyperthermophilic bacterium was isolated from pink filamentous streamers (pink filaments) occurring in the upper outflow channel (temperature, 82 to 88°C) of Octopus Spring in Yellowstone National Park, Wyo. The gram-negative cells grew at low salinity at temperatures up to 89°C in the neutral to alkaline pH range. Depending on the culture conditions, the organisms occurred as single motile rods, as aggregates, or as long filaments that formed streamer-like cell masses. The novel isolate grew chemolithoautotrophically with hydrogen, thiosulfate, and elemental sulfur as electron donors and oxygen as the electron acceptor. Alternatively, under aerobic conditions, formate and formamide served as sole energy and carbon sources. The novel isolate had a 16S rRNA sequence closely related to the 16S rRNA sequence obtained from uncultivated pink filaments. It represents a new genus in the orderAquificales, the type species of which we nameThermocrinis ruber (type strain, OC 1/4 [= DSM 12173]).

scholarly journals Phylogeny of the Defined Murine Microbiota: Altered Schaedler Flora

1999 ◽  
Vol 65 (8) ◽  
pp. 3287-3292 ◽  
Author(s):  
Floyd E. Dewhirst ◽  
Chih-Ching Chien ◽  
Bruce J. Paster ◽  
Rebecca L. Ericson ◽  
Roger P. Orcutt ◽  
...  
Keyword(s):  
16S Rrna ◽  
Single Species ◽  
Rapid Identification ◽  
Sequence Information ◽  
Rrna Sequence ◽  
Gram Positive ◽  
16S Rrna Sequence ◽  
16S Rrna Analysis ◽  
Rrna Sequences ◽  
16S Rrna Sequences

ABSTRACT The “altered Schaedler flora” (ASF) was developed for colonizing germfree rodents with a standardized microbiota. The purpose of this study was to identify each of the eight ASF strains by 16S rRNA sequence analysis. Three strains were previously identified asLactobacillus acidophilus (strain ASF 360),Lactobacillus salivarius (strain ASF 361), andBacteroides distasonis (strain ASF 519) based on phenotypic criteria. 16S rRNA analysis indicated that each of the strains differed from its presumptive identity. The 16S rRNA sequence of strain ASF 361 is essentially identical to the 16S rRNA sequences of the type strains of Lactobacillus murinis and Lactobacillus animalis (both isolated from mice), and all of these strains probably belong to a single species. Strain ASF 360 is a novel lactobacillus that clusters with L. acidophilus andLactobacillus lactis. Strain ASF 519 falls into an unnamed genus containing [Bacteroides] distasonis, [Bacteroides] merdae, [Bacteroides] forsythus, and CDC group DF-3. This unnamed genus is in theCytophaga-Flavobacterium-Bacteroides phylum and is most closely related to the genus Porphyromonas. The spiral-shaped strain, strain ASF 457, is in the Flexistipesphylum and exhibits sequence identity with rodent isolates of Robertson. The remaining four ASF strains, which are extremely oxygen-sensitive fusiform bacteria, group phylogenetically with the low-G+C-content gram-positive bacteria (Firmicutes,Bacillus-Clostridium group). ASF 356, ASF 492, and ASF 502 fall into Clostridium cluster XIV of Collins et al. Morphologically, ASF 492 resembles members of this cluster,Roseburia cecicola, and Eubacterium plexicaudatum. The 16S rRNA sequence of ASF 492 is identical to that of E. plexicaudatum. Since the type strain and other viable original isolates of E. plexicaudatum have been lost, strain ASF 492 is a candidate for a neotype strain. Strain ASF 500 branches deeply in the low-G+C-content gram-positive phylogenetic tree but is not closely related to any organisms whose 16S rRNA sequences are currently in the GenBank database. The 16S rRNA sequence information determined in the present study should allow rapid identification of ASF strains and should permit detailed analysis of the interactions of ASF organisms during development of intestinal disease in mice that are coinfected with a variety of pathogenic microorganisms.

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