A highly sensitive fluorescence method for the detection of T4 polynucleotide kinase phosphatase based on polydopamine nanotubes

2021 ◽  
pp. 120594
Author(s):  
Tuo Zhang ◽  
Yanmei Shen ◽  
Jia Ge ◽  
Weixia Wang ◽  
Lingbo Qu ◽  
...  
Keyword(s):  
Fluorescence Method ◽  
Highly Sensitive ◽  
Polynucleotide Kinase ◽  
T4 Polynucleotide Kinase

A novel fluorescence method for the highly sensitive detection of T4 polynucleotide kinase based on polydopamine nanotubes

2019 ◽  
Vol 43 (42) ◽  
pp. 16753-16758 ◽  
Author(s):  
Xue-Ping Shen ◽  
Jia Ge ◽  
Juan Chen ◽  
Yan-Mei Shen ◽  
Hong-Min Meng ◽  
...  
Keyword(s):  
Fluorescence Method ◽  
Sensitive Detection ◽  
Highly Sensitive ◽  
Polynucleotide Kinase ◽  
Highly Sensitive Detection ◽  
T4 Polynucleotide Kinase

A novel fluorescence method has been developed for the detection of T4 PNK using FRET between dye-labeled ssDNA and PDANTs.

A fluorescence-based assay for T4 polynucleotide kinase/phosphatase activity based on a terminal transferase-aided photoinduced electron transfer strategy

2016 ◽  
Vol 8 (9) ◽  
pp. 1989-1994 ◽  
Author(s):  
Changbei Ma ◽  
Shunxin Jin ◽  
Jun Wang ◽  
Kemin Wang ◽  
Haisheng Liu ◽  
...  
Keyword(s):  
Electron Transfer ◽  
Phosphatase Activity ◽  
Photoinduced Electron Transfer ◽  
Fluorescence Method ◽  
Terminal Transferase ◽  
Polynucleotide Kinase ◽  
T4 Polynucleotide Kinase

A novel fluorescence method for detection of T4 polynucleotide kinase/phosphatase activity based on a terminal transferase-aided photoinduced electron transfer strategy.

A WS2 nanosheet based sensing platform for highly sensitive detection of T4 polynucleotide kinase and its inhibitors

Nanoscale ◽  
2014 ◽  
Vol 6 (12) ◽  
pp. 6866-6872 ◽  
Author(s):  
Jia Ge ◽  
Li-Juan Tang ◽  
Qiang Xi ◽  
Xi-Ping Li ◽  
Ru-Qin Yu ◽  
...  
Keyword(s):  
Sensitive Detection ◽  
Sensing Platform ◽  
Highly Sensitive ◽  
Polynucleotide Kinase ◽  
Highly Sensitive Detection ◽  
T4 Polynucleotide Kinase

A nanobiosensor was developed based on a WS2 nanosheet for detection of T4 polynucleotide kinase with low interference and superior sensitivity.

Development of a highly sensitive sensing platform for T4 polynucleotide kinase phosphatase and its inhibitors based on WS2 nanosheets

2014 ◽  
Vol 6 (18) ◽  
pp. 7212-7217 ◽  
Author(s):  
Xiaomeng Liu ◽  
Jia Ge ◽  
Xiangyu Wang ◽  
Zhan Wu ◽  
Guoli Shen ◽  
...  
Keyword(s):  
Phosphatase Activity ◽  
Activity Assay ◽  
Sensing Platform ◽  
Highly Sensitive ◽  
Polynucleotide Kinase ◽  
Novel Strategy ◽  
Ws2 Nanosheets ◽  
Nucleotide Kinase ◽  
T4 Polynucleotide Kinase

A novel strategy for nucleotide kinase phosphatase activity assay based on WS2 nanosheets.

Sensitive and label-free T4 polynucleotide kinase/phosphatase detection based on poly(thymine)-templated copper nanoparticles coupled with nicking enzyme-assisted signal amplification

2016 ◽  
Vol 8 (13) ◽  
pp. 2831-2836 ◽  
Author(s):  
Jia Ge ◽  
Lin Zhang ◽  
Zhen-Zhen Dong ◽  
Qi-Yong Cai ◽  
Zhao-Hui Li
Keyword(s):  
Copper Nanoparticles ◽  
Signal Amplification ◽  
Label Free ◽  
Free T4 ◽  
Highly Sensitive ◽  
Polynucleotide Kinase ◽  
Nicking Enzyme ◽  
T4 Polynucleotide Kinase

A novel label-free biosensor for highly sensitive sensing of the activity as well as inhibition of T4 PNKP based on poly T-templated fluorescent CuNPs and nicking enzyme-assisted signal amplification.

A novel fluorescence method for activity assay and drug screening of T4 PNK by coupling rGO with ligase reaction

The Analyst ◽  
2019 ◽  
Vol 144 (4) ◽  
pp. 1187-1196 ◽  
Author(s):  
Hongyan Zhou ◽  
Chunyi Tong ◽  
Wei Zou ◽  
Yupei Yang ◽  
Yongbei Liu ◽  
...  
Keyword(s):  
Drug Screening ◽  
Fluorescence Method ◽  
Hydroxyl Group ◽  
Activity Assay ◽  
Kinase Family ◽  
Polynucleotide Kinase ◽  
T4 Polynucleotide Kinase

T4 polynucleotide kinase (PNK) is the primary member of the 5′-kinase family that can transfer the γ-phosphate residue of ATP to the 5′-hydroxyl group of oligonucleotides.

Sign in / Sign up

Export Citation Format

Share Document