In vitro regeneration of Guizotia abyssinica Cass. and evaluation of genetic fidelity through RAPD markers

The present study reports a simple protocol for in vitro regeneration of Aglaonema ‘Valentine’ using axillary shoot explants for rapid multiplication and production of true-to-type plants. Different concentrations of benzyladenine (BA; 0, 1, 3, 5, and 7 mg·L−1), kinetin (Kin; 0, 1, 3, 5, and 7 mg·L−1), thidiazuron (TDZ; 0, 0.5, 1.0, 1.5, and 2.0 mg·L−1), naphthalene acetic acid (NAA; 0, 0.5, and 1.0 mg·L−1), and indole-3-butyric acid (IBA; 0, 0.5, and 1.0 mg·L−1) were used for shoot regeneration. The highest shoot proliferation (5.0) was obtained on Murashige and Skoog (MS) medium supplemented with 1.5 mg·L−1 TDZ and 1 mg·L−1 NAA. In vitro rooting was easily achieved with 100% at all concentrations of NAA and IBA supplemented to half- or full-strength MS medium. Regenerated plantlets were acclimatized in greenhouse with 100% survival rate. Randomly amplified polymorphic DNA (RAPD) analysis confirmed the genetic fidelity of the regenerated plantlets and mother plant.

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In vitro regeneration and PCR-RAPD based detection of somaclonal variation in kenaf (Hibiscus cannabinus)

Progressive Agriculture ◽

10.3329/pa.v28i2.33470 ◽

2017 ◽

Vol 28 (2) ◽

pp. 100-108

Author(s):

MS Haque ◽

T Biswas ◽

MS Islam ◽

MS Hossain

Keyword(s):

Somaclonal Variation ◽

Rapd Markers ◽

In Vitro Regeneration ◽

Random Amplified Polymorphic Dna ◽

Hibiscus Cannabinus ◽

Petiole Explants ◽

Napthaleneacetic Acid ◽

Mother Plants ◽

Regenerated Plantlets

Though direct systems of regeneration through culture of organized meristems usually produce true-to-type plants, variations in the progenies have widely been reported. Fiber producing kenaf plants (Hibiscus cannabinus L.) were regenerated from petiole, hypocotyls and cotyledonous petiole explants on MS medium containing BAP (benzyl amino purine) and NAA (?-napthaleneacetic acid) followed by assessment of regenerants by RAPD markers to detect somaclonal variation among them. Genomic DNA from twenty seven plants [three mother plants and two clones (clone 1 and 2) from each mother plant with three replications] was subjected to random amplified polymorphic DNA (RAPD) analysis. Fifteen polymorphic loci amplified by three decamer random primers were used to estimate genetic diversity and relatedness in mother plants and their regenerated plantlets. The results showed some degree of polymorphism between mother plants and their regenerated plantlets as well as between regenerated plantlets indicating somaclonal variation among the regenerants. These suggest that the RAPD technique could effectively be used to detect somaclonal variation in H. cannabinus and could be promising for the detection of markers associated with desirable traits.Progressive Agriculture 28 (2): 100-108, 2017

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In vitro regeneration of wild pear (Pyrus pyraster Burgsd) clones tolerant to Fe-chlorosis and somaclonal variation analysis by RAPD markers

Plant Cell Reports ◽

10.1007/s00299-006-0256-9 ◽

2006 ◽

Vol 26 (4) ◽

pp. 489-496 ◽

Cited By ~ 15

Author(s):

Maria Antonietta Palombi ◽

Beatrice Lombardo ◽

Emilia Caboni

Keyword(s):

Somaclonal Variation ◽

Rapd Markers ◽

In Vitro Regeneration ◽

Variation Analysis ◽

Fe Chlorosis ◽

Pyrus Pyraster

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In vitro regeneration and assessment of genetic fidelity of acclimated plantlets by using ISSR markers in PPR-1 (Morus sp.): An economically important plant

Scientia Horticulturae ◽

10.1016/j.scienta.2018.07.012 ◽

2018 ◽

Vol 241 ◽

pp. 313-321 ◽

Cited By ~ 16

Author(s):

Gulab Khan Rohela ◽

Phanikanth Jogam ◽

Aftab Ahmad Shabnam ◽

Pawan Shukla ◽

Sadanandam Abbagani ◽

...

Keyword(s):

In Vitro Regeneration ◽

Genetic Fidelity ◽

Issr Markers

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In Vitro Regeneration and ISSR-Based Genetic Fidelity Analysis of Orthosiphon stamineus Benth

Agronomy ◽

10.3390/agronomy9120778 ◽

2019 ◽

Vol 9 (12) ◽

pp. 778 ◽

Cited By ~ 2

Author(s):

Hanisah Ali ◽

Izzah Farhanah Musa ◽

Nurul Atikhah Abu Bakar ◽

Saiful Anuar Karsani ◽

Jamilah Syafawati Yaacob

Keyword(s):

In Vitro Regeneration ◽

Genetic Fidelity ◽

Epileptic Seizures ◽

Black Soil ◽

Inter Simple Sequence Repeat ◽

Issr Markers ◽

Direct Regeneration ◽

Nodal Segment ◽

Orthosiphon Stamineus

Orthosiphon stamineus has been widely used as traditional remedy for various illnesses and diseases, such as cardiovascular diseases and epileptic seizures. In this study, direct regeneration through nodal segment of this species was attempted using Kinetin (6-Furfurylaminopurine) and IAA (indole-3-acetic acid). Optimum regeneration media was identified as MS media supplemented with 2.0 mg L−1 Kin plus 0.5 mg L−1 IAA. This yielded the highest number of shoots (5.57 ± 0.42) and leaves (20.53 ± 1.91) per explant. Acclimatization of the resulting in vitro regenerants was successful in all potting mixtures tested. However, potting mixture PF (1:1:1 ratio of black soil/red soil/compost) was identified as the best medium for acclimatization of this species, as it yielded 100% survival percentage after 90 days of acclimatization. Ten in vitro regenerants of O. stamineus were randomly collected after the third subculture and subjected to genetic variation analysis using inter-simple sequence repeat (ISSR) markers. Out of 20 ISSR markers tested, 10 working primers were observed to produce satisfactory amplification of bands, with an average of 7.11 bands per primer. A total of 610 bands were produced by the 10 primers. The percentage of polymorphism was observed to be very low, yielding only 7.32% polymorphism among all samples. Jaccard dissimilarity analysis was also conducted and very low genetic distance (about 0.1) was found among the in vitro regenerants and between the regenerants with the mother plant, thus ascertaining the clonal nature of the plantlets produced in this study.

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