Coverage degrees of colloids on electrochemical electrodes and signal amplification for anti-citrullinated peptide antibody detection

OBJECTIVES: To compare the sensitivity and specificity of serum anti-Cyclic Citrullinated Peptideantibody (anti-CCP) with serum rheumatoid factor in diagnosing rheumatoid arthritis (RA) in acommunity hospital in Lahore, Pakistan.DESIGN: Cross-sectional analytical study.PLACE & DURATION OF STUDY: Subjects were recruited from Fatima Memorial Hospital,Rheumatology Outpatient Department from January, 2010 to December, 2010. The research work wasconducted at Department of Physiology and Cell Biology of University of Health Sciences, Lahore.SUBJECTS & METHODS: Eighty diagnosed patients of rheumatoid arthritis and thirty normalhealthy controls were included in the study. After selection of subjects, written informed consent wasobtained. The venous blood sample was taken and secured in vacutainers. Serum was extracted bycentrifugation and stored at -20°C till analysis. Titers of anti-CCP and rheumatoid factor weredetermined by ELISA. The data obtained was analyzed by using SPSS version 16.0.RESULTS: The sensitivity and specificity of serum anti-CCP was found to be 58.6% and 86.7%respectively as compared to 47.5% and 83.3% for serum rheumatoid factor (RF) in diagnosingrheumatoid arthritis. Sensitivity of anti-CCP antibody in RF negative sub-group was 32.1%.CONCLUSION: Serum anti-CCP antibody is more sensitive and specific marker for diagnosis ofrheumatoid arthritis as compared to the rheumatoid factor.KEY WORDS: Rheumatoid Arthritis, Anti- Cyclic Citrullinated Peptide Antibody (ACCP), Rheumatoid Factor

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The findings of musculoskeletal ultrasonography on primary Sjögren’s syndrome patients in childhood with articular manifestations and the impact of anti-cyclic citrullinated peptide antibody

Modern Rheumatology ◽

10.1080/14397595.2018.1530849 ◽

2018 ◽

Vol 29 (5) ◽

pp. 821-828

Author(s):

Kosuke Shabana ◽

Nami Okamoto ◽

Yuko Sugita ◽

Keisuke Shindo ◽

Takuji Murata ◽

...

Keyword(s):

Sjogren’S Syndrome ◽

Sjogren's Syndrome ◽

Primary Sjögren’S Syndrome ◽

Primary Sjogren’S Syndrome ◽

Primary Sjögren's Syndrome ◽

Cyclic Citrullinated Peptide ◽

Peptide Antibody ◽

Musculoskeletal Ultrasonography ◽

The Impact ◽

Citrullinated Peptide

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OTOİMMÜN ROMATOİD HASTALIKLARDA OTOANTİKORLAR

Euroasia Journal of Mathematics, Engineering, Natural and Medical Sciences ◽

10.38065/euroasiaorg.627 ◽

2021 ◽

Vol 8 (17) ◽

pp. 135-140

Author(s):

Nuray GÜREL-POLAT

Keyword(s):

Western Blot ◽

Clinical Medicine ◽

Standard Test ◽

Immunological Tests ◽

Peptide Antibody ◽

Golden Standard ◽

Organ Specific ◽

Definition Of ◽

Citrullinated Peptide ◽

Rheumatoid Diseases

The diagnosis of autoimmune rheumatoid diseases can be made by combining parameters consisting of clinical, histopathological, laboratory and immunological tests. Among these parameters, autoantibodies are of great benefit in differential diagnosis, especially for patients with unclear clinical data. Immunofluorescence, ELISA, immunodiffusion, immunoprecipitation and Western blot can be used to identify autoantibodies. While autoantibodies like antinukleer antibody, double stranded deoksiribonukleic asid and anti neutrophils antibody are detected by immunofluorescence as a golden standard test, Anti-cyclic Citrullinated Peptide Antibody (CCP), antiphospholipid antibodies (anti-cardiolipid) are evaluated by ELISA and ENA group by immunoblot or western blot. Today the number of autoantibodies that can be detected is over 100. The indication of some of these autoantibodies are not known even today. The definition of antinuclear antibody group autoantibodies plays a crucial role for the diagnosis and treatment of systemic and organ-specific illnesses. With these methods, practical, fast and trustworthy results in clinical medicine and clinical immunology can be obtained.

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