Rapid in vitro propagation of Dendrobium hybrids through direct shoot formation from foliar explants, and protocorm-like bodies

The leaves of vegetative stolons of greenhouse grown Cryptanthus `Marian Oppenheimer' (wide leaf clone) were cultured in modified MS media to induce adventitious shoot formation via callus formation. The best callus induction medium was basal MS medium with 10 μM NAA, IBA and BA. Pure green (843), maroon (3), striped (2) and albino plantlets were obtained. Most of the albino plantlets were stunted, tightly clumped together and impossible to score. The medium which produced the highest average number of non-albino plantlets was basal MS medium with 0.3 μM NAA, IBA and BA All non-albino plantlets were rooted in MS medium with 5.4 μM NAA and transplanted ex vitro with a survival rate of 96.7%. The maroon plantlets became green two weeks after transplanting. Histological studies revealed that C. `Marian Oppenheimer' (wide leaf clone) has two tunicas (L1 and L2) and a corpus (L3). Callus on the leaf explant arose mainly from the L2 and L3. Apparently C. `Marian Oppenheimer' (wide leaf clone) is a GWG periclinal chimera.

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The creation of a Taraxacum kok-saghyz high frequency regeneration system

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/843/1/012041 ◽

2021 ◽

Vol 843 (1) ◽

pp. 012041

Author(s):

A A Verbitskaya ◽

A S Ivanova ◽

N G Konkova ◽

A K Gaponenko

Keyword(s):

Culture Medium ◽

Ph Value ◽

Culture Media ◽

Shoot Formation ◽

High Survival ◽

Regeneration System ◽

Hormone Exposure ◽

Direct Shoot Formation ◽

Root Tissues

Abstract The aim of this research was to study the morphogenetic ability of Taraxacum kok-saghyz root tissues and to optimize the culture medium for the subsequent genetic transformation of plants. The effects of exogenous hormone exposure on survival, in vitro shoot induction, and root formation were studied by using root tissues. For the cultivation the samples of kok-saghyz, the Murashige-Skuga nutrient medium was used as the basis, supplemented with sucrose 20 g/l, vitamins B5 1 mg/l, and also containing agar 5.5 g/l. The pH value is 5.7. For the plant regeneration induction, growth regulators, auxins and cytokinins were added in culture media. In this study, the roots of D 1 mm and two media variants were used for comparison: variant 1. MS + 6-BAP 1 mg/l; variant 2. MS + 6-BAP 1 mg/l + IAA 0.2 mg/l. An effective protocol for the regeneration of kok-saghyz explants was developed. There was a high percentage of regeneration of 87.6% on the medium containing a combination of cytokinin and auxin, as well as a high percentage of direct shoot formation, which was 65.1%, the degree of rooting was 100%, the resulting cultured plant tissues grew well and had a high survival rate after transplantation.

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Effectiveness of Daphne L. (Thymelaeaceae) in vitro propagation, rooting of microshoots and acclimatization of plants

Acta Agrobotanica ◽

10.5586/aa.2012.039 ◽

2012 ◽

Vol 65 (1) ◽

pp. 21-28 ◽

Cited By ~ 4

Author(s):

Ewa Hanus-Fajerska ◽

Alina Wiszniewska ◽

Przemysław Czaicki

Keyword(s):

Plant Material ◽

In Vitro Propagation ◽

Shoot Formation ◽

Mineral Salts ◽

Shrub Species ◽

Multiplication Coefficient ◽

Shoot Propagation ◽

Propagation Media

In this study, an attempt was made to investigate in vitro morphogenetic competence of three shrub species from the Thymelaeaceae family. The studied plant material originated from Russia, Greece and China, and the effectiveness of in vitro shoot formation and rhizogenesis of Daphne caucasica, D. jasminea, and D. tangutica was verified. The multiplication coefficient was compared for different propagation media. Medium composed of WPM mineral salts, MS microelements and a set of vitamins, supplemented with 1.0 mg dm-3 2iP, 0.1 mg dm-3 NAA, and 0.65 g dm-3 calcium gluconate, was appropriate for micropropagation of the tested genotypes. Shoot propagation in medium containing B5 vitamins and microelements was not as effective as on WPM/MS medium. The rooting phase, especially in D. tangutica, needs further optimization in order to reduce the costs associated with acclimatization of microplantlets obtained to in vivo conditions. After stabilization, the plants were successfully cultivated under greenhouse conditions.

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