scholarly journals CALLUS INDUCTION AND DIRECT SHOOT FORMATION IN IN VITRO CULTURED IMMATURE INFLORESCENCE TISSUES OF COCONUT

COCOS ◽  
2010 ◽  
Vol 12 (0) ◽  
pp. 39 ◽  
Author(s):  
V. R. M. VIDHANA ARACHCHI ◽  
L. K. WEERAKOON
Keyword(s):  
Callus Induction ◽  
Shoot Formation ◽  
Immature Inflorescence ◽  
Direct Shoot Formation ◽  
Direct Shoot

scholarly journals IN VITRO REGENERATION AND MASS PROPAGATION OF AZIMA TETRACANTHA [LAM.] FROM THE LEAF EXPLANTS THROUGH CALLUS CULTURE

2017 ◽  
Vol 4 (2) ◽  
pp. 52-56
Author(s):  
Mallika Devi T
Keyword(s):  
Callus Induction ◽  
In Vitro Regeneration ◽  
Leaf Explants ◽  
Shoot Formation ◽  
Ms Medium ◽  
Apical Leaf ◽  
Half Strength ◽  
Regenerated Plantlets ◽  
Azima Tetracantha

In the present study the protocol for callus induction and regeneration in Azima tetracantha has been developed in culture medium. The young apical leaf explants were used for callus induction on MS medium containing BAP and NAA at 1.0 and 0.4mgl-1 respectively showed maximum callus induction (73%). The amount of callus responded for shoot formation (74%) was obtained in the MS medium containing BAP (1.5 mgl-1) and NAA (0.3mgl-1).The elongated shoots were rooted on half strength medium supplemented with IBA (1.5 mgl-1) and Kn (0.4 mgl-1) for shoots rooted. Regenerated plantlets were successfully acclimatized and hardened off inside the culture and then transferred to green house with better survival rate.

scholarly journals The creation of a Taraxacum kok-saghyz high frequency regeneration system

2021 ◽  
Vol 843 (1) ◽  
pp. 012041
Author(s):  
A A Verbitskaya ◽  
A S Ivanova ◽  
N G Konkova ◽  
A K Gaponenko
Keyword(s):  
Culture Medium ◽  
Ph Value ◽  
Culture Media ◽  
Shoot Formation ◽  
High Survival ◽  
Regeneration System ◽  
Hormone Exposure ◽  
Direct Shoot Formation ◽  
Root Tissues

Abstract The aim of this research was to study the morphogenetic ability of Taraxacum kok-saghyz root tissues and to optimize the culture medium for the subsequent genetic transformation of plants. The effects of exogenous hormone exposure on survival, in vitro shoot induction, and root formation were studied by using root tissues. For the cultivation the samples of kok-saghyz, the Murashige-Skuga nutrient medium was used as the basis, supplemented with sucrose 20 g/l, vitamins B5 1 mg/l, and also containing agar 5.5 g/l. The pH value is 5.7. For the plant regeneration induction, growth regulators, auxins and cytokinins were added in culture media. In this study, the roots of D 1 mm and two media variants were used for comparison: variant 1. MS + 6-BAP 1 mg/l; variant 2. MS + 6-BAP 1 mg/l + IAA 0.2 mg/l. An effective protocol for the regeneration of kok-saghyz explants was developed. There was a high percentage of regeneration of 87.6% on the medium containing a combination of cytokinin and auxin, as well as a high percentage of direct shoot formation, which was 65.1%, the degree of rooting was 100%, the resulting cultured plant tissues grew well and had a high survival rate after transplantation.

Direct shoot formation and plant regeneration from cotyledon explants of rapid-cycling Brassica rapa

1997 ◽  
Vol 33 (4) ◽  
pp. 288-292 ◽  
Author(s):  
Winnie Teo ◽  
Prakash Lakshmanan ◽  
Prakash Kumar ◽  
Chong-Jin Goh ◽  
Sanjay Swarup
Keyword(s):  
Plant Regeneration ◽  
Brassica Rapa ◽  
Shoot Formation ◽  
Rapid Cycling ◽  
Cotyledon Explants ◽  
Direct Shoot Formation ◽  
Direct Shoot

scholarly journals Efficient Plant Regeneration of Ipomoea aquatica by Direct Shoot Formation from Cotyledon Segments.

2001 ◽  
Vol 18 (1) ◽  
pp. 77-79 ◽  
Author(s):  
Ancharida AKARACHARANYA ◽  
Young-Eui CHOI ◽  
Tomonobu KUSANO ◽  
Atsuhiko SHINMYO ◽  
Hiroshi SANO
Keyword(s):  
Plant Regeneration ◽  
Shoot Formation ◽  
Ipomoea Aquatica ◽  
Direct Shoot Formation ◽  
Direct Shoot
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