In vitro conservation of citrus rootstocks using paclobutrazol and analysis of plant viability and genetic stability

2021 ◽  
Vol 286 ◽  
pp. 110231
Author(s):  
Maria Inês de Souza Mendes ◽  
Denise dos Santos Vila Verde ◽  
Andresa Priscila de Souza Ramos ◽  
Abelmon da Silva Gesteira ◽  
Walter dos Santos Soares Filho ◽  
...  
Keyword(s):  
Genetic Stability ◽  
In Vitro Conservation

Viability and genetic stability of pineapple germplasm after 10 years of in vitro conservation

2016 ◽  
Vol 127 (1) ◽  
pp. 123-133 ◽  
Author(s):  
Ronilze Leite da Silva ◽  
Claudia Fortes Ferreira ◽  
Carlos Alberto da Silva Ledo ◽  
Everton Hilo de Souza ◽  
Paulo Henrique da Silva ◽  
...  
Keyword(s):  
Genetic Stability ◽  
In Vitro Conservation

Effects of low temperature on Chrysanthemum shiwogiku var. kinokuniense in vitro conservation

2018 ◽  
Vol 44 (4) ◽  
pp. 675-678
Author(s):  
Weilan ◽  
Shucheng Xu ◽  
Xuelan Zhu
Keyword(s):  
Plant Regeneration ◽  
Low Temperature ◽  
Genetic Stability ◽  
Test Tube ◽  
In Vitro Conservation ◽  
Young Plant ◽  
In Vitro Plantlets ◽  
Surviving Rate ◽  
Issr Pcr

The effect of low temperature condition on wild Chrysanthemum shiwogiku var. in vitro conservation was investigated, and its plant regeneration hereditary stability was detected using its sterile seedling. The results show that the test tube young plant grows rapidly, and its preservation time is short at a temperature at 25±2°C, at condition of 12 h/d, photoperiod at 2000 ~ 3000 lx, and all die after 180 days. However, the sterile seedling grows slowly under low-temperature, and its preservation time extends to 360 days. The sterile seedling surviving rate was above 96%. The data indicate that the 4°C low temperature is advantageous to Chrysanthemum plantlets preservation. After preservation, the recovered plantlets grow well and show no differences in morphology and isoenzyme zymogram of peroxidase, ISSR-PCR compared with the control. In addition, the results show that low temperature in vitro plantlets maintain genetic stability.

scholarly journals Genetic identity of mint cultivars after in vitro conservation, assessed by ISSR primers

2021 ◽  
Vol 937 (4) ◽  
pp. 042014
Author(s):  
M S Zagorskaya ◽  
S F Abdurashytov
Keyword(s):  
Genetic Stability ◽  
Culture Media ◽  
Callus Formation ◽  
In Vitro Conservation ◽  
Artificial Culture ◽  
Full Compliance ◽  
Conservation Genetic ◽  
Somaclonal Variability ◽  
Issr Primers

Abstract The species of the genus Mentha have been known since ancient times and have significant value in the pharmaceutical, cosmetic and food industries, as well as in medicine. For the widespread use of mint, including in a variety of breeding programs, and the preservation of genetic diversity, effective methods of maintaining cultivars and collection samples are required. Thanks to the development of biotechnological methods, in particular, the creation of slow-growing collections, are now actively used as an effective alternative to field collections. It is known that the cultivation of tissues and organs on artificial culture media can cause somaclonal variability. The purpose of this work is to study the effect of in vitro storage at 4-6°C without illumination after 3 and 4 in vitro conservation cycles on the genetic stability of three cultivars of mint Azhurnaya, Bergamotnaya and Zagrava using ISSR primers. 1 cycle: 1 year of in vitro conservation, microcutting and 2 subcultures of regrowth in a culture room. After conservation, the number of viable explants was 70.0-82.1%. Callus formation at the base of the shoots was not observed in any of the cultivars. After 3 and 4 cycles of in vitro conservation, genetic stability was assessed using 11 ISSR primers. It was found that all three mint genotypes showed full compliance (length and number of amplicons) with the profiles of control samples for all studied markers. It was also found that the markers used by ISSR are highly informative for mint cultivars.

scholarly journals In vitro Conservation and Cryopreservation of Nandina domestica, an Outdoor Ornamental Shrub

2013 ◽  
Vol 41 (2) ◽  
pp. 638 ◽  
Author(s):  
Aylin OZUDOGRU ◽  
Diogo Pedrosa Corrêa Da SILVA ◽  
Ergun KAYA ◽  
Giuliano DRADI ◽  
Renato PAIVA ◽  
...  
Keyword(s):  
Liquid Nitrogen ◽  
Storage Temperature ◽  
Sucrose Concentration ◽  
Aluminum Foil ◽  
Shoot Tips ◽  
In Vitro Conservation ◽  
Shoot Cultures ◽  
Storage Medium ◽  
Droplet Vitrification

The study focused on an economically-important ornamental outdoor shrub, Nandina domestica, with the aims to (i) optimize an effective in vitro conservation method, and (ii) develop a cryopreservation protocol for shoot tips by the PVS2 vitrification and droplet-vitrification techniques. For in vitro conservation of shoot cultures, the tested parameters were sucrose content in the storage medium (30, 45, 60 g/L) and storage temperature (4 °C or 8 °C). Cryopreservation was performed by applying the PVS2 vitrification solution, in 2-ml cryovials or in drops over aluminum foil strips, for 15, 30, 60 or 90 min at 0 °C, followed by the direct immersion in liquid nitrogen of shoot tips. Results show that N. domestica shoots can be conserved successfully for 6 months at both the temperatures tested, especially when 60 g/L sucrose is used in the storage medium. However, conservation at 4 °C showed to be more appropriate, as hyperhydricity was observed in post-conservation of shoots coming from storage at 8 °C. As for cryopreservation, a daily gradual increase of sucrose concentration (from 0.25 to 1.0 M) produced better protection to the samples that were stored in liquid nitrogen. Indeed, with this sucrose treatment method, a 30-min PVS2 incubation time was enough to produce, 60 days after thawing, the best recovery (47% and 50%) of shoot tips, cryopreserved with PVS2 vitrification and droplet-vitrification, respectively.

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