Effects of low temperature on Chrysanthemum shiwogiku var. kinokuniense in vitro conservation

2018 ◽  
Vol 44 (4) ◽  
pp. 675-678
Author(s):  
Weilan ◽  
Shucheng Xu ◽  
Xuelan Zhu
Keyword(s):  
Plant Regeneration ◽  
Low Temperature ◽  
Genetic Stability ◽  
Test Tube ◽  
In Vitro Conservation ◽  
Young Plant ◽  
In Vitro Plantlets ◽  
Surviving Rate ◽  
Issr Pcr

The effect of low temperature condition on wild Chrysanthemum shiwogiku var. in vitro conservation was investigated, and its plant regeneration hereditary stability was detected using its sterile seedling. The results show that the test tube young plant grows rapidly, and its preservation time is short at a temperature at 25±2°C, at condition of 12 h/d, photoperiod at 2000 ~ 3000 lx, and all die after 180 days. However, the sterile seedling grows slowly under low-temperature, and its preservation time extends to 360 days. The sterile seedling surviving rate was above 96%. The data indicate that the 4°C low temperature is advantageous to Chrysanthemum plantlets preservation. After preservation, the recovered plantlets grow well and show no differences in morphology and isoenzyme zymogram of peroxidase, ISSR-PCR compared with the control. In addition, the results show that low temperature in vitro plantlets maintain genetic stability.

Growth, anatomy and histochemistry of fast growing species under in vitro conservation through mineral oil and low-temperature combination

2020 ◽  
Author(s):  
Luciana Florêncio de Lacerda ◽  
Hugo Teixeira Gomes ◽  
Patrícia Monah Cunha Bartos ◽  
Jaqueline Martins Vasconcelos ◽  
Sebastião Carvalho Vasconcelos Filho ◽  
...  
Keyword(s):  
Low Temperature ◽  
Mineral Oil ◽  
In Vitro Conservation ◽  
Fast Growing ◽  
Fast Growing Species

scholarly journals Medium Term Conservation of Several Carnation Accessions Via in Vitro Culture

2012 ◽  
Vol 13 (2) ◽  
pp. 174
Author(s):  
Kurniawan Budiarto ◽  
Budi Marwoto
Keyword(s):  
Low Temperature ◽  
Significant Variation ◽  
Root Formation ◽  
In Vitro Conservation ◽  
Medium Term ◽  
Low Temperature Storage ◽  
Two Factors ◽  
Temperature Storage

Sufficient genetic diversity is important in carnation breeding program. In vivo conservation of carnation germplasmis considered inefficient due to some technical and economical aspects. In vitro conservation was then, expectedto overcome the limitation of in vivo method. The research was conducted to find out the proper media for medium-term in vitro conservation of several carnation accessions in low temperature storage. A complete factorialexperiment with 25 replications was designed to accomplish the combination of two factors. The first factor wassix commercial carnation cultivars, namely Pink Maladi, Orange Triumph, Opera, Tundra, Yellow Liberty and PradoReffit. The second factor was the conservation media i.e. 1⁄2MS + DMSO 3% and 1⁄2MS + 3% DMSO + 3% sucrose andcontrol (MS 0+3% sucrose). The results showed that in vitro conservation of carnation in low temperature weresuccessfully conducted using 1⁄2MS+3% DMSO and 1⁄2MS+3% DMSO+3% sucrose without significant variation in allaccessions tested up to 10 and 12 months respectively. The increase of death plantlets, however, was detected onthe media of 1⁄2MS+3% DMSO after 6 months storage with significant decrease in viability hereafter. The existenceof sucrose in DMSO media induced root formation and plantlet resistance to low temperature storage.

Plant Regeneration from Pea Leaflets Cultured in vitro and Genetic Stability of Regenerants

1984 ◽  
Vol 117 (2) ◽  
pp. 119-130 ◽  
Author(s):  
A. Rubluo ◽  
K.K. Kartha ◽  
L.A. Mroginski ◽  
J. Dyck
Keyword(s):  
Plant Regeneration ◽  
Genetic Stability

Viability and genetic stability of pineapple germplasm after 10 years of in vitro conservation

2016 ◽  
Vol 127 (1) ◽  
pp. 123-133 ◽  
Author(s):  
Ronilze Leite da Silva ◽  
Claudia Fortes Ferreira ◽  
Carlos Alberto da Silva Ledo ◽  
Everton Hilo de Souza ◽  
Paulo Henrique da Silva ◽  
...  
Keyword(s):  
Genetic Stability ◽  
In Vitro Conservation

scholarly journals Genetic identity of mint cultivars after in vitro conservation, assessed by ISSR primers

2021 ◽  
Vol 937 (4) ◽  
pp. 042014
Author(s):  
M S Zagorskaya ◽  
S F Abdurashytov
Keyword(s):  
Genetic Stability ◽  
Culture Media ◽  
Callus Formation ◽  
In Vitro Conservation ◽  
Artificial Culture ◽  
Full Compliance ◽  
Conservation Genetic ◽  
Somaclonal Variability ◽  
Issr Primers

Abstract The species of the genus Mentha have been known since ancient times and have significant value in the pharmaceutical, cosmetic and food industries, as well as in medicine. For the widespread use of mint, including in a variety of breeding programs, and the preservation of genetic diversity, effective methods of maintaining cultivars and collection samples are required. Thanks to the development of biotechnological methods, in particular, the creation of slow-growing collections, are now actively used as an effective alternative to field collections. It is known that the cultivation of tissues and organs on artificial culture media can cause somaclonal variability. The purpose of this work is to study the effect of in vitro storage at 4-6°C without illumination after 3 and 4 in vitro conservation cycles on the genetic stability of three cultivars of mint Azhurnaya, Bergamotnaya and Zagrava using ISSR primers. 1 cycle: 1 year of in vitro conservation, microcutting and 2 subcultures of regrowth in a culture room. After conservation, the number of viable explants was 70.0-82.1%. Callus formation at the base of the shoots was not observed in any of the cultivars. After 3 and 4 cycles of in vitro conservation, genetic stability was assessed using 11 ISSR primers. It was found that all three mint genotypes showed full compliance (length and number of amplicons) with the profiles of control samples for all studied markers. It was also found that the markers used by ISSR are highly informative for mint cultivars.

Sign in / Sign up

Export Citation Format

Share Document