Research to determine the ripening time of the fruit is the scientific basis for better harvesting and preservation. Physiological and biochemical methods were used to analyze the changes of some indicators according to the growth and development of banana fruit grown in Thanh Liet commune, Thanh Tri district, Hanoi from the time of its formation until the fruit ripening. The results showed that the banana reached the maximum size at 16 weeks old, at this time the peel was yellow due to the decrease in chlorophyll and increased carotenoid content. The content of vitamin C and total organic acid content reached their maximum when the fruit at 12 weeks old, then decreased gradually. Starch content increased to 14 weeks old, then decreased. Reduced sugar content increased gradually to 16 weeks old and then decreased. Protein content decreased gradually from fruit formation until fruit ripening, lipid content increased gradually to 15 weeks old, then decreased. Through the research process, we have determined that the physiological ripe time of banana fruit was 16 weeks old, this is the time when the fruit stops growing and accumulates many nutrients.