Enhanced bioremediation of nutrient-amended, petroleum hydrocarbon-contaminated soils over a cold-climate winter: The rate and extent of hydrocarbon biodegradation and microbial response in a pilot-scale biopile subjected to natural seasonal freeze-thaw temperatures

2018 ◽  
Vol 612 ◽  
pp. 903-913 ◽  
Author(s):  
Jihun Kim ◽  
Aslan Hwanhwi Lee ◽  
Wonjae Chang
Keyword(s):  
Contaminated Soils ◽  
Petroleum Hydrocarbon ◽  
Pilot Scale ◽  
Cold Climate ◽  
Hydrocarbon Biodegradation ◽  
Freeze Thaw ◽  
Microbial Response ◽  
Enhanced Bioremediation

scholarly journals Petroleum Hydrocarbon Biodegradation under Seasonal Freeze−Thaw Soil Temperature Regimes in Contaminated Soils from a Sub-Arctic Site

2011 ◽  
Vol 45 (3) ◽  
pp. 1061-1066 ◽  
Author(s):  
Wonjae Chang ◽  
Sara Klemm ◽  
Chantale Beaulieu ◽  
Jalal Hawari ◽  
Lyle Whyte ◽  
...  
Keyword(s):  
Soil Temperature ◽  
Contaminated Soils ◽  
Petroleum Hydrocarbon ◽  
Hydrocarbon Biodegradation ◽  
Freeze Thaw ◽  
Temperature Regimes

Assessment of five bioaccessibility assays for predicting the efficacy of petroleum hydrocarbon biodegradation in aged contaminated soils

Chemosphere ◽  
2010 ◽  
Vol 81 (9) ◽  
pp. 1061-1068 ◽  
Author(s):  
Catherine E. Dandie ◽  
John Weber ◽  
Samuel Aleer ◽  
Eric M. Adetutu ◽  
Andy S. Ball ◽  
...  
Keyword(s):  
Contaminated Soils ◽  
Petroleum Hydrocarbon ◽  
Hydrocarbon Biodegradation

scholarly journals Experimental Investigation of a Pilot-Scale Concerning Ex-Situ Bioremediation of Petroleum Hydrocarbons Contaminated Soils

2021 ◽  
Vol 13 (15) ◽  
pp. 8165
Author(s):  
Valer Micle ◽  
Ioana Monica Sur
Keyword(s):  
Contaminated Soils ◽  
Petroleum Hydrocarbons ◽  
Petroleum Hydrocarbon ◽  
Industrial Area ◽  
Pilot Scale ◽  
Soil Samples ◽  
Point Of View ◽  
Ex Situ ◽  
Nutrient Contents ◽  
Aeration Time

The soil samples were taken from the site of a former oil products depot from an industrial area (Romania). The soil samples taken were analyzed from a physical and chemical point of view: texture, pH, soil micronutrient content, metals concentration and petroleum hydrocarbon concentration (PHCs). The soil contaminated with total petroleum hydrocarbon (TPH (4280 mg kg−1) was disposed in the form of a pile (L × W × H: 3000 × 1400 × 500 mm). Experiments on a pilot-scale were conducted over 12 weeks at constant pH (7.5–8), temperature (22–32 °C), nutrient contents C/N/P ratio 100/10/1, soil aeration time (8 h/day) and moisture (30%). Samples were taken every two weeks for the monitoring of the TPH and the microorganisms content. During the experiment, microorganisms were added (Pseudomonas and Bacillus) every two weeks. Results of the analyses regarding the concentration of PHCs were revealed a linear decrease of the concentration of PHCs after only two weeks of treatment. This decrease in concentration was also achieved in the following weeks. Following the analysis performed on the model at the pilot scale regarding the depollution process, it can be concluded that a soil contaminated with petroleum hydrocarbons can be efficiently depolluted by performing an aeration of 8 h/day, adding microorganisms Pseudomonas and Bacillus to ensure the conditions for increasing in the total number of germs (colony forming units–CFU) from 151 × 105 to 213 × 107 CFU g−1 soil, after 12 weeks of soil treatment—the depollution efficiency achieved is 83%.

scholarly journals Unravelling the process of petroleum hydrocarbon biodegradation in different filter materials of constructed wetlands by stable isotope fractionation and labelling studies

2021 ◽  
Author(s):  
Andrea Watzinger ◽  
Melanie Hager ◽  
Thomas Reichenauer ◽  
Gerhard Soja ◽  
Paul Kinner
Keyword(s):  
Stable Isotope ◽  
Constructed Wetlands ◽  
Hydrogen Isotope ◽  
Petroleum Hydrocarbon ◽  
Isotope Fractionation ◽  
Isotope Effects ◽  
Filter Material ◽  
Hydrocarbon Biodegradation ◽  
Stable Isotope Fractionation ◽  
Filter Materials

AbstractMaintaining and supporting complete biodegradation during remediation of petroleum hydrocarbon contaminated groundwater in constructed wetlands is vital for the final destruction and removal of contaminants. We aimed to compare and gain insight into biodegradation and explore possible limitations in different filter materials (sand, sand amended with biochar, expanded clay). These filters were collected from constructed wetlands after two years of operation and batch experiments were conducted using two stable isotope techniques; (i) carbon isotope labelling of hexadecane and (ii) hydrogen isotope fractionation of decane. Both hydrocarbon compounds hexadecane and decane were biodegraded. The mineralization rate of hexadecane was higher in the sandy filter material (3.6 µg CO2 g−1 day−1) than in the expanded clay (1.0 µg CO2 g−1 day−1). The microbial community of the constructed wetland microcosms was dominated by Gram negative bacteria and fungi and was specific for the different filter materials while hexadecane was primarily anabolized by bacteria. Adsorption / desorption of petroleum hydrocarbons in expanded clay was observed, which might not hinder but delay biodegradation. Very few cases of hydrogen isotope fractionation were recorded in expanded clay and sand & biochar filters during decane biodegradation. In sand filters, decane was biodegraded more slowly and hydrogen isotope fractionation was visible. Still, the range of observed apparent kinetic hydrogen isotope effects (AKIEH = 1.072–1.500) and apparent decane biodegradation rates (k = − 0.017 to − 0.067 day−1) of the sand filter were low. To conclude, low biodegradation rates, small hydrogen isotope fractionation, zero order mineralization kinetics and lack of microbial biomass growth indicated that mass transfer controlled biodegradation.

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